메뉴 건너뛰기




Volumn 54, Issue 3, 2013, Pages 155-164

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

Author keywords

MicroRNAs; RT qPCR; Short RNA enrichment

Indexed keywords

MICRORNA;

EID: 84876466682     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114002     Document Type: Article
Times cited : (109)

References (47)
  • 1
    • 4644309196 scopus 로고    scopus 로고
    • The functions of anim microR NAs
    • Ambros, V. 2004. The functions of anim microR NAs. Nature 431:350-355.
    • (2004) Nature , vol.431 , pp. 350-355
    • Ambros, V.1
  • 2
    • 33748928159 scopus 로고    scopus 로고
    • The diverse functions of microRNAs in anima development and disease
    • Kloosterman, W. P. and R.H. Plasterk. 2006 The diverse functions of microRNAs in anima development and disease. Dev. Cell 11:441-450.
    • (2006) Dev. Cell , vol.11 , pp. 441-450
    • Kloosterman, W.P.1    Plasterk, R.H.2
  • 3
    • 0034673638 scopus 로고    scopus 로고
    • An RNA-directed nucle mediates post-transcriptional gene silencing in Drosophila cells
    • Hammond, S.M., E. Bernstein, D. Beach, and G.J. Hannon. 2000. An RNA-directed nucle mediates post-transcriptional gene silencing in Drosophila cells. Nature 404:293-296.
    • (2000) Nature , vol.404 , pp. 293-296
    • Hammond, S.M.1    Bernstein, E.2    Beach, D.3    Hannon, G.J.4
  • 4
    • 0037031576 scopus 로고    scopus 로고
    • Single-stranded antisensesi RNA s guide target RNA cleavage in RNA i
    • Marti nez, J., A. Patkaniowska, H. Urlaub, R. Luhrmann, and T. Tuschl. 2002. Single-stranded antisensesi RNA s guide target RNA cleavage in RNA i. Cell 110:563-574.
    • (2002) Cell , vol.110 , pp. 563-574
    • Marti nez, J.1    Patkaniowska, A.2    Urlaub, H.3    Luhrmann, R.4    Tuschl, T.5
  • 6
    • 0036752955 scopus 로고    scopus 로고
    • Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
    • Schwarz, D. S., G. Hutvagner, B. Haley, and P.D. Zamore. 2002. Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol. Cell 10:537-548.
    • (2002) Mol. Cell , vol.10 , pp. 537-548
    • Schwarz, D.S.1    Hutvagner, G.2    Haley, B.3    Zamore, P.D.4
  • 9
    • 53349177819 scopus 로고    scopus 로고
    • Characterization of microRNAs in serum: A novel class of biomarkers for diagnosis of cancer and other diseases
    • Chen, X., Ba, Y., Ma, L., Cai, X., Yin, Y., Wang, K., Guo, J., Zhang, Y., et al. 2008. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cel l Res. 18:997-1006.
    • (2008) Cel L Res , vol.18 , pp. 997-1006
    • Chen, X.1    Ba, Y.2    Ma, L.3    Cai, X.4    Yin, Y.5    Wang, K.6    Guo, J.7    Zhang, Y.8
  • 10
    • 57649091633 scopus 로고    scopus 로고
    • The detection of differentially expressed microR NAs from the ser u m of ovarian cancer patient s using a novel real-time PCR platform
    • Resnick, K.E., H. Alder, J.P. Hagan, D.L. Richardson, C.M. Croce, and D.E. Cohn. 2009. The detection of differentially expressed microR NAs from the ser u m of ovarian cancer patients using a novel real-time P CR platform. Gynecol. Oncol. 112:55-59.
    • (2009) Gynecol. Oncol , vol.112 , pp. 55-59
    • Resnick, K.E.1    Alder, H.2    Hagan, J.P.3    Richardson, D.L.4    Croce, C.M.5    Cohn, D.E.6
  • 11
    • 52049121293 scopus 로고    scopus 로고
    • Mature miR-18 4 a s Potential Oncogenic microRNA of Squamous Cell Carcinoma of Tongue
    • Wong, T. S., X.B. L i u, B.Y. Wong, R.W. Ng, A.P. Yue n, and W. I. Wei. 2008. Mature miR-18 4 a s Potential Oncogenic microRNA of Squamous Cel l Carcinoma of Tongue. Clin. Cancer Res. 14: 2588-2592.
    • (2008) Clin. Cancer Res , vol.14 , pp. 2588-2592
    • Wong, T.S.1    Liu, X.B.2    Wong, B.Y.3    Ng, R.W.4    Yuen, A.P.5    Wei, W.I.6
  • 12
    • 66049112674 scopus 로고    scopus 로고
    • Differential expression of microRNAs inplasma of patients with colorectal cancer: A potential marker for colorectal cancer screening
    • Ng, E.K., W.W. Chong, H. Jin, E.K. Lam, V.Y. Shin, J. Yu, T.C. Poon, S. S. Ng, and J.J. Su ng. 2009. Differential expression of microRNAs inplasma of patients with colorectal cancer: a potential marker for colorecta l cancer screening. Gut 58:1375-1381.
    • (2009) G Ut , vol.58 , pp. 1375-1381
    • Ng, E.K.1    Chong, W.W.2    Jin, H.3    Lam, E.K.4    Shin, V.Y.5    Yu, J.6    Poon, T.C.7    Ng, S.S.8    Ng, J.J.9    Ng, S.U.10
  • 13
    • 77954161288 scopus 로고    scopus 로고
    • Circulating microR NA: A novel potential biomarker for early diagnosis of acute myocardial infarction in humans
    • Wang, G. K., J.Q. Z hu, J.T. Zhang, Q. L i, Y. L i, J. H e, Y.W. Qin, and Q. Jing. 2010. Circulating microR NA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans. Eur. Heart J. 3 1:659-666.
    • (2010) Eur. Heart J , vol.3 , Issue.1 , pp. 659-666
    • Wang, G.K.1    Hu, J.Q.Z.2    Zhang, J.T.3    Li, Q.4    Li, Y.5    He, J.6    Qin, Y.W.7    Jing, Q.8
  • 14
    • 77957052771 scopus 로고    scopus 로고
    • Circulating microR NAs as potential biomarkers of coronaryartery disease: A promise to be fulfilled?
    • Contu, R., M.V. Latronico, and G. Condorelli. 2010. Circulating microR NAs as potential biomarkers of coronaryartery disease: a promise to be fulfilled? Circ. Res. 107:573-574.
    • (2010) Circ. Res , vol.107 , pp. 573-574
    • Contu, R.1    Latronico, M.V.2    Condorelli, G.3
  • 15
    • 1942494040 scopus 로고    scopus 로고
    • Expression profiling of mammalian microRNs u ncovers a subset of brain-expressed microRNAs with possible roles inmurine and human neuronal differentiation
    • Sempere, L.F., S. Freemantle, I. Pitha-Rowe, E. Moss, E. Dmitrovsky, and V. Ambros. 2004. Expression profiling of mammalian microRNs u ncovers a subset of brain-expressed microRNAs with possible roles inmurine and human neuronal differentiation. Genome B iol. 5:R13.
    • (2004) Genome B Iol , vol.5
    • Sempere, L.F.1    Freemantle, S.2    Pitha-Rowe, I.3    Moss, E.4    Dmitrovsky, E.5    Ambros, V.6
  • 16
    • 79952109548 scopus 로고    scopus 로고
    • Construction of small RNA c DNA libraries for deep sequencing
    • Thomas, M. F. and K. M. Ansel. 2010. Construction of small RNA c DN A librarie s for deep sequencing. Method s Mol. Biol. 667: 93-111.
    • (2010) Method S Mol. Biol , vol.667 , pp. 93-111
    • Thomas, M.F.1    Ansel, K.M.2
  • 21
    • 30344456035 scopus 로고    scopus 로고
    • Facile means for quantifying microRNA expression by real-time PCR
    • Shi, R. and V. L. Chiang 2005. Facile means for quantifying microRNA expression by real-time PCR. Biotechniques 39:519-525.
    • (2005) Biotechniques , vol.39 , pp. 519-525
    • Shi, R.1    Chiang, V.L.2
  • 22
    • 51349111360 scopus 로고    scopus 로고
    • Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer
    • Davoren, P.A., R.E. McNeill, A.J. Lowery, M.J. Kerin, and N. Miller. 2008. Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer. BMC Mol. Biol. 9: 76.
    • (2008) BMC Mol. Biol , vol.9 , pp. 76
    • Davoren, P.A.1    McNeill, R.E.2    Lowery, A.J.3    Kerin, M.J.4    Miller, N.5
  • 24
    • 78149433644 scopus 로고    scopus 로고
    • A pilot study of circulating miRNAs as potential biomarkers of early stage breast cancer
    • Zhao, H., J. Shen, L. Medico, D. Wang, C.B. Ambrosone, and S. Liu. 2010 A pilot study of circulating miRNAs as potential biomarkers of early stage breast cancer. PLoS ONE 5:e13735.
    • (2010) PLoS ONE , vol.5
    • Zhao, H.1    Shen, J.2    Medico, L.3    Wang, D.4    Ambrosone, C.B.5    Liu, S.6
  • 25
    • 77649201708 scopus 로고    scopus 로고
    • Expression profiling of microRNA using real-time quantitative PCR, how to use it and what is available
    • Benes, V. and M. Castoldi. 2010. Expression profiling of microRNA using real-time quantitative PCR, how to use it and what is available. Methods 50:244-249.
    • (2010) Methods , vol.50 , pp. 244-249
    • Benes, V.1    Castoldi, M.2
  • 26
    • 84867619796 scopus 로고    scopus 로고
    • Potential pitfalls in microRNA profiling
    • Chugh, P. and D. P. Dittmer. 2012. Potential pitfalls in microRNA profiling. WIREs RNA 3: 601-616.
    • (2012) WIREs R NA , vol.3 , pp. 601-616
    • Chugh, P.1    Dittmer, D.P.2
  • 27
    • 55049126795 scopus 로고    scopus 로고
    • Focus on RNA isolation: Obtaining RNA for micro RNA (miRNA) expression profiling analyses of neural tissue
    • Wang, W.X., B.R. Wilfred, D.A. Baldwin, R.B. Isett, N. Ren, A. Stromberg, and P.T. Nelson. 2008. Focus on R N A is o la t ion: obtaining RNA for micro RNA (miRNA) expression profiling analyses of neural tissue. Biochim. Biophys.Acta 1779:749-757.
    • (2008) Biochim. Biophys. Acta , vol.1779 , pp. 749-757
    • Wang, W.X.1    Wilfred, B.R.2    Baldwin, D.A.3    Isett, R.B.4    Ren, N.5    Stromberg, A.6    Nelson, P.T.7
  • 28
    • 52449110023 scopus 로고    scopus 로고
    • Measuring microRNAs: Comparisons of micro array and quantitative PCR measurements, and of different total RNA prep methods
    • Ach, R.A., H. Wang, and B. Curry. 2008. Measuring microRNAs: comparison s of micro array and quantitative PCR measurements, and of different total RNA prep methods. BMC Biotechnol. 8: 69.
    • (2008) BMC Biotechnol , vol.8 , pp. 69
    • Ach, R.A.1    Wang, H.2    Curry, B.3
  • 31
    • 72249098584 scopus 로고    scopus 로고
    • Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses
    • Hammerle-Fickinger, A., I. Riedmaier, C. Becker, H.H. Meyer, M.W. Pfaffl, and S.E. Ulbrich. 2010. Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses. Biotechnol. Lett. 32:35-44
    • (2010) Biotechnol. Lett , vol.32 , pp. 35-44
    • Hammerle-Fickinger, A.1    Riedmaier, I.2    Becker, C.3    Meyer, H.H.4    Pfaffl, M.W.5    Ulbrich, S.E.6
  • 32
    • 84867237573 scopus 로고    scopus 로고
    • Method for microRNA isolation from clinical serum samples
    • Li, Y. and K.V. Kowdley. 2012. Method for microRNA isolation from clinical serum samples. Anal. Biochem. 431:69-75.
    • (2012) Anal. Biochem , vol.431 , pp. 69-75
    • Li, Y.1    Kowdley, K.V.2
  • 33
    • 84858330248 scopus 로고    scopus 로고
    • Importance of RNA isolation methods for analysis of exosomal RNA: Evaluation of different methods
    • Eldh, M., J. Lötvall, C. Malmhäll, and K. Ekström. 2012. Importance of RNA isolation methods for analysis of exosomal RNA: evaluation of different methods. Mol. Immunol. 50: 278-286.
    • (2012) Mol. Immunol , vol.50 , pp. 278-286
    • Eldh, M.1    Lötvall, J.2    Malmhäll, C.3    Ekström, K.4
  • 34
    • 42449098100 scopus 로고    scopus 로고
    • Normalization of microRNA expression levels in quantitative RT- PCR assays: Identification of suitable reference RNA targets in normal and cancerous human solid tissues
    • Peltier, H.J. and G.J. Latham. 2008. Normalization of microRNA expression levels in quantitative RT- PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues. RN 14:844-852.
    • (2008) RN , vol.14 , pp. 844-852
    • Peltier, H.J.1    Latham, G.J.2
  • 37
    • 77649205818 scopus 로고    scopus 로고
    • Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-P C R (qRT-PC R)
    • Kroh E.M., R.K. Parkin, P. S. Mitchell, and M. Tewari. 2010. Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-P C R (qRT-PC R). Methods 50:298-301.
    • (2010) Methods , vol.50 , pp. 298-301
    • Kroh, E.M.1    Parkin, R.K.2    Mitchell, P.S.3    Tewari, M.4
  • 38
    • 80051779652 scopus 로고    scopus 로고
    • Plasma miRNA as biomarkers for assesment of total-body radiation exposure dosimetry
    • Cui, W., J. Ma, Y. Wang, and S. Biswal. 2011. Plasma miRNA as biomarkers for assesment of total-body radiation exposure dosimetry. PLoS ONE 6:e22988.
    • (2011) PLoS ONE , vol.6
    • Cui, W.1    Ma, J.2    Wang, Y.3    Biswal, S.4
  • 40
    • 33845599045 scopus 로고    scopus 로고
    • Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers
    • Levin, J. D., D. Fiala, M.F. Samala, J.D. Kahn, and R.J. Peterson. 2006. Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers. Nucleic Acids Res. 34: e142.
    • (2006) Nucleic Acids Res , vol.34
    • Levin, J.D.1    Fiala, D.2    Samala, M.F.3    Kahn, J.D.4    Peterson, R.J.5
  • 41
    • 70350445696 scopus 로고    scopus 로고
    • Reciprocal regulation of microRNA and m RNA profiles in neuronal development and synapse formation
    • Manakov, S.A., S.G. Grant, and A.J. Enright. 2009. Reciprocal regulation of m icroRNA and m RNA profiles in neuronal development and synapse formation. BMC Genomics 10: 419.
    • (2009) BMC Genomics , vol.10 , pp. 419
    • Manakov, S.A.1    Grant, S.G.2    Enright, A.J.3
  • 42
    • 81555212307 scopus 로고    scopus 로고
    • MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample type sinorals quamous cell carcinoma
    • Wiklund, E.D., Gao, S., Hulf, T., Sibbritt, T., Nair, S., Costea, D.E., Villadsen, S.B., Bakholdt, V., et al. 2011. MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample type sinorals quamous cell carcinoma. PLoSONE 6:e27840.
    • (2011) PLoSONE , vol.6
    • Wiklund, E.D.1    Gao, S.2    Hulf, T.3    Sibbritt, T.4    Nair, S.5    Costea, D.E.6    Villadsen, S.B.7    Bakholdt, V.8
  • 43
  • 46
    • 34548687914 scopus 로고    scopus 로고
    • Construction of small RNA cDNA libraries for deep sequencing
    • Lu C., B.C. Meyers, and P.J. Green. 2007. Construction of small RNA cDNA libraries for deep sequencing. Methods 43:110-117.
    • (2007) Methods , vol.43 , pp. 110-117
    • Lu, C.1    Meyers, B.C.2    Green, P.J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.