Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses

Biotechnology Letters

Volumn 32, Issue 1, 2010, Pages 35-44

  Hammerle Fickinger, Andrea ^a   Riedmaier, Irmgard ^a   Becker, Christiane ^a   Meyer, Heinrich H D ^a   Pfaffl, Michael W ^a   Ulbrich, Susanne E ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Blood; Bovine; miRNA; Molecular diagnosis; mRNA; qPCR

Indexed keywords

BLOOD; DIAGNOSIS; GENE EXPRESSION; MAMMALS; MOLECULAR BIOLOGY; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; RNA;

BOVINE; MIRNA; MOLECULAR DIAGNOSIS; MRNA; QPCR;

EXTRACTION;

BOVINAE;

EID: 72249098584     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0130-2     Document Type: Article

References (13)

1. Ahmadi MR, Nazifi S, Ghaisari HR (2006) Comparison of hormonal changes of estrous cycle with cytology of cervical mucosa and hematological parameters in dairy heifers. Comp Clin Path 15(2): 94-97.
2. Bustin SA, Benes V, Nolan T, Pfaffl MW (2005) Quantitative real-time RT-PCR-a perspective. J Mol Endocrinol 34(3): 597-601.
3. Feezor RJ, Baker HV, Mindrinos M, Hayden D, Tannahill CL, Brownstein BH, Fay A, MacMillan S, Laramie J, Xiao W, Moldawer LL, Cobb JP, Laudanski K, Miller-Graziano CL, Maier RV, Schoenfeld D, Davis RW, Tompkins RG (2004) Whole blood and leukocyte RNA isolation for gene expression analyses. Physiol Genomics 19(3): 247-254.
4. Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 27(2-3): 126-139.
5. Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW (2006) Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett 28(19): 1601-1613.
6. Kruhoffer M, Dyrskjot L, Voss T, Lindberg RL, Wyrich R, Thykjaer T, Orntoft TF (2007) Isolation of microarray-grade total RNA, microRNA, and DNA from a single PAXgene blood RNA tube. J Mol Diagn 9(4): 452-458.
7. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9): e45.
8. Rainen L, Oelmueller U, Jurgensen S, Wyrich R, Ballas C, Schram J, Herdman C, Bankaitis-Davis D, Nicholls N, Trollinger D, Tryon V (2002) Stabilization of mRNA expression in whole blood samples. Clin Chem 48(11): 1883-1890.
9. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3(6): 1101-1108.
10. Shivdasani RA (2006) MicroRNAs: regulators of gene expression and cell differentiation. Blood 108(12): 3646-3653.
11. Stefani G, Slack FJ (2008) Small non-coding RNAs in animal development. Nat Rev Mol Cell Biol 9(3): 219-230.
12. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22(1): 130-138.
13. Wright C, Bergstrom D, Dai H, Marton M, Morris M, Tokiwa G, Wang Y, Fare T (2008) Characterization of globin RNA interference in gene expression profiling of whole-blood samples. Clin Chem 54(2): 396-405.