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Volumn 17, Issue 1, 2013, Pages 51-58

New options to engineer biofuel microbes: Development and application of a high-throughput screening system

Author keywords

Butanol; Clostridium; Ethanol; Nitroblue tetrazolium

Indexed keywords

BIOSYNTHETIC ACTIVITIES; CLOSTRIDIUM ACETOBUTYLICUM; COMBINATORIAL STRATEGIES; DEVELOPMENT AND APPLICATIONS; HIGH-THROUGHPUT SCREENING SYSTEMS; METABOLIC ENGINEERING APPROACH; NITROBLUE TETRAZOLIUM; SOLVENTOGENIC CLOSTRIDIA;

EID: 84876397731     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.03.002     Document Type: Article
Times cited : (25)

References (53)
  • 2
    • 79952910616 scopus 로고    scopus 로고
    • Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways
    • Bond-Watts B.B., Bellerose R.J., Chang M.C.Y. Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways. Nat. Chem. Biol. 2011, 7:222-227.
    • (2011) Nat. Chem. Biol. , vol.7 , pp. 222-227
    • Bond-Watts, B.B.1    Bellerose, R.J.2    Chang, M.C.Y.3
  • 3
    • 34248196414 scopus 로고    scopus 로고
    • Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum
    • Borden J.R., Papoutsakis E.T. Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum. Appl. Environ. Microbiol. 2007, 73:3061-3068.
    • (2007) Appl. Environ. Microbiol. , vol.73 , pp. 3061-3068
    • Borden, J.R.1    Papoutsakis, E.T.2
  • 4
    • 0000754708 scopus 로고
    • Physicochemical factors in growth
    • American Society for Microbiology, Washington, DC, USA, P. Gerhardt, R.G.E. Murray, W.A. Wood, N.R. Krieg (Eds.)
    • Breznak J.A., Costilow R.N. Physicochemical factors in growth. Methods for General and Molecular Bacteriology 1994, American Society for Microbiology, Washington, DC, USA. P. Gerhardt, R.G.E. Murray, W.A. Wood, N.R. Krieg (Eds.).
    • (1994) Methods for General and Molecular Bacteriology
    • Breznak, J.A.1    Costilow, R.N.2
  • 5
    • 0024588512 scopus 로고
    • Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase (EC 2.8.3.9) and in other solvent pathway enzymes
    • Clark S.W., Bennett G.N., Rudolph F.B. Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase (EC 2.8.3.9) and in other solvent pathway enzymes. Appl. Environ. Microbiol. 1989, 55:970-976.
    • (1989) Appl. Environ. Microbiol. , vol.55 , pp. 970-976
    • Clark, S.W.1    Bennett, G.N.2    Rudolph, F.B.3
  • 6
    • 84869014233 scopus 로고    scopus 로고
    • Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-9ethanol fermentation pathway
    • Cooksley C.M., Zhang Y., Wang H., Redl S., Winzer K., Minton N.P. Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-9ethanol fermentation pathway. Metab. Eng. 2012, 14:630-641.
    • (2012) Metab. Eng. , vol.14 , pp. 630-641
    • Cooksley, C.M.1    Zhang, Y.2    Wang, H.3    Redl, S.4    Winzer, K.5    Minton, N.P.6
  • 7
    • 0032986060 scopus 로고    scopus 로고
    • Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum
    • Desai R.P., Papoutsakis E.T. Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl. Environ. Microbiol. 1999, 65:936-945.
    • (1999) Appl. Environ. Microbiol. , vol.65 , pp. 936-945
    • Desai, R.P.1    Papoutsakis, E.T.2
  • 8
    • 77953642931 scopus 로고    scopus 로고
    • High-throughput metabolic engineering: advances in small-molecule screening and selection
    • Dietrich J.A., McKee A.E., Keasling J.D. High-throughput metabolic engineering: advances in small-molecule screening and selection. Ann. Rev. Biochem. 2010, 79:563-590.
    • (2010) Ann. Rev. Biochem. , vol.79 , pp. 563-590
    • Dietrich, J.A.1    McKee, A.E.2    Keasling, J.D.3
  • 9
    • 0022494501 scopus 로고
    • Treatment with allyl alcohol selects specifically for mutants of Clostridium acetobutylicum defective in butanol synthesis
    • Dürre P., Kuhn A., Gottschalk G. Treatment with allyl alcohol selects specifically for mutants of Clostridium acetobutylicum defective in butanol synthesis. FEMS Microbiol. Lett. 1986, 36:77-81.
    • (1986) FEMS Microbiol. Lett. , vol.36 , pp. 77-81
    • Dürre, P.1    Kuhn, A.2    Gottschalk, G.3
  • 10
    • 0027477339 scopus 로고
    • Colorimetric assay to determine alcohol dehydrogenase activity
    • Fibla J., Gonzàlez-Duarte R. Colorimetric assay to determine alcohol dehydrogenase activity. J. Biochem. Biophys. Methods 1993, 26:87-93.
    • (1993) J. Biochem. Biophys. Methods , vol.26 , pp. 87-93
    • Fibla, J.1    Gonzàlez-Duarte, R.2
  • 11
    • 0036180998 scopus 로고    scopus 로고
    • Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824
    • Fontaine L., Meynial-Salles I., Girbal L., Yang X., Croux C., Soucaille P. Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J. Bacteriol. 2002, 184:821-830.
    • (2002) J. Bacteriol. , vol.184 , pp. 821-830
    • Fontaine, L.1    Meynial-Salles, I.2    Girbal, L.3    Yang, X.4    Croux, C.5    Soucaille, P.6
  • 12
    • 79958010538 scopus 로고    scopus 로고
    • Fermentative production of butanol-the industrial perspective
    • Green E.M. Fermentative production of butanol-the industrial perspective. Curr. Opin. Biotechnol. 2011, 22:337-343.
    • (2011) Curr. Opin. Biotechnol. , vol.22 , pp. 337-343
    • Green, E.M.1
  • 15
    • 84860367333 scopus 로고    scopus 로고
    • Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios
    • Hönicke D., Janssen H., Grimmler C., Ehrenreich A., Lütke-Eversloh T. Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios. N. Biotechnol. 2012, 29:485-493.
    • (2012) N. Biotechnol. , vol.29 , pp. 485-493
    • Hönicke, D.1    Janssen, H.2    Grimmler, C.3    Ehrenreich, A.4    Lütke-Eversloh, T.5
  • 16
    • 84863012205 scopus 로고    scopus 로고
    • Butanol production from renewable biomass: Rediscovery of metabolic pathways and metabolic engineering
    • Jang Y.S., Lee J., Malaviya A., Seung D.Y., Cho J.H., Lee S.Y. Butanol production from renewable biomass: Rediscovery of metabolic pathways and metabolic engineering. Biotechnol. J. 2012, 7:186-198.
    • (2012) Biotechnol. J. , vol.7 , pp. 186-198
    • Jang, Y.S.1    Lee, J.2    Malaviya, A.3    Seung, D.Y.4    Cho, J.H.5    Lee, S.Y.6
  • 18
    • 84876750152 scopus 로고    scopus 로고
    • Acetone-butanol-ethanol production with high productivity using Clostridium acetobutylicum BKM19
    • Jang Y.S., Malaviya A., Lee S.Y. Acetone-butanol-ethanol production with high productivity using Clostridium acetobutylicum BKM19. Biotechnol. Bioeng. 2013, 10.1002/bit.24843.
    • (2013) Biotechnol. Bioeng.
    • Jang, Y.S.1    Malaviya, A.2    Lee, S.Y.3
  • 19
    • 68049142960 scopus 로고    scopus 로고
    • Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio
    • Jiang Y., Xu C., Dong F., Yang Y., Jiang W., Yang S. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab. Eng. 2009, 11:284-291.
    • (2009) Metab. Eng. , vol.11 , pp. 284-291
    • Jiang, Y.1    Xu, C.2    Dong, F.3    Yang, Y.4    Jiang, W.5    Yang, S.6
  • 20
    • 0022970603 scopus 로고
    • Acetone-butanol fermentation revisited
    • Jones D.T., Woods D.R. Acetone-butanol fermentation revisited. Microbiol. Rev. 1986, 50:484-524.
    • (1986) Microbiol. Rev. , vol.50 , pp. 484-524
    • Jones, D.T.1    Woods, D.R.2
  • 21
    • 70449129501 scopus 로고    scopus 로고
    • Strain improvement and process development for biobutanol production
    • Kharkwal S., Karimi I.A., Chang M.W., Lee D.Y. Strain improvement and process development for biobutanol production. Rec. Pat. Biotechnol. 2009, 3:202-210.
    • (2009) Rec. Pat. Biotechnol. , vol.3 , pp. 202-210
    • Kharkwal, S.1    Karimi, I.A.2    Chang, M.W.3    Lee, D.Y.4
  • 22
    • 84877696851 scopus 로고    scopus 로고
    • ClosTron-mediated engineering of Clostridium
    • Kuehne S.A., Minton N.P. ClosTron-mediated engineering of Clostridium. Bioeng. Bugs 2012, 3:247-254.
    • (2012) Bioeng. Bugs , vol.3 , pp. 247-254
    • Kuehne, S.A.1    Minton, N.P.2
  • 23
    • 70449575862 scopus 로고    scopus 로고
    • Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production
    • Lee J.Y., Jang Y.S., Lee J., Papoutsakis E.T., Lee S.Y. Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol. J. 2009, 4:1432-1440.
    • (2009) Biotechnol. J. , vol.4 , pp. 1432-1440
    • Lee, J.Y.1    Jang, Y.S.2    Lee, J.3    Papoutsakis, E.T.4    Lee, S.Y.5
  • 24
    • 84876382177 scopus 로고    scopus 로고
    • Neue Einblicke in den Gärungsstoffwechsel von Clostridium acetobutylicum.
    • Rostock University, Germany.
    • Lehmann, D., 2012. Neue Einblicke in den Gärungsstoffwechsel von Clostridium acetobutylicum. Ph.D. Thesis. Rostock University, Germany.
    • (2012) Ph.D. Thesis.
    • Lehmann, D.1
  • 25
  • 26
    • 80052029042 scopus 로고    scopus 로고
    • Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway
    • Lehmann D., Lütke-Eversloh T. Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway. Metab. Eng 2011, 13:464-473.
    • (2011) Metab. Eng , vol.13 , pp. 464-473
    • Lehmann, D.1    Lütke-Eversloh, T.2
  • 27
    • 84871045858 scopus 로고    scopus 로고
    • New insights into the butyric acid metabolism of Clostridium acetobutylicum
    • Lehmann D., Radomski N., Lütke-Eversloh T. New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 2012, 96:1325-1339.
    • (2012) Appl. Microbiol. Biotechnol. , vol.96 , pp. 1325-1339
    • Lehmann, D.1    Radomski, N.2    Lütke-Eversloh, T.3
  • 28
    • 0022383354 scopus 로고
    • Mutagenesis in Clostridium acetobutylicum
    • Lemmel S.A. Mutagenesis in Clostridium acetobutylicum. Biotechnol. Lett. 1985, 7:711-716.
    • (1985) Biotechnol. Lett. , vol.7 , pp. 711-716
    • Lemmel, S.A.1
  • 30
    • 77955141315 scopus 로고    scopus 로고
    • Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
    • Linhová M., Patáková P., Lipovsky J., Fribert P., Paulová L., Rychtera M., Melzoch K. Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum. Folia Microbiol. 2010, 55:340-344.
    • (2010) Folia Microbiol. , vol.55 , pp. 340-344
    • Linhová, M.1    Patáková, P.2    Lipovsky, J.3    Fribert, P.4    Paulová, L.5    Rychtera, M.6    Melzoch, K.7
  • 31
    • 80052625837 scopus 로고    scopus 로고
    • Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production
    • Lütke-Eversloh T., Bahl H. Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production. Curr. Opin. Biotechnol. 2011, 22:634-647.
    • (2011) Curr. Opin. Biotechnol. , vol.22 , pp. 634-647
    • Lütke-Eversloh, T.1    Bahl, H.2
  • 32
    • 84865750105 scopus 로고    scopus 로고
    • Over-expression of stress protein-encoding genes helps Clostridium acetobutylicum to rapidly adapt to butanol stress
    • Mann M.S., Dragovic Z., Schirrmacher G., Lütke-Eversloh T. Over-expression of stress protein-encoding genes helps Clostridium acetobutylicum to rapidly adapt to butanol stress. Biotechnol. Lett. 2012, 34:1643-1649.
    • (2012) Biotechnol. Lett. , vol.34 , pp. 1643-1649
    • Mann, M.S.1    Dragovic, Z.2    Schirrmacher, G.3    Lütke-Eversloh, T.4
  • 33
    • 84872665471 scopus 로고    scopus 로고
    • Thiolase engineering for enhanced butanol production in Clostridium acetobutylicum
    • Mann M.S., Lütke-Eversloh T. Thiolase engineering for enhanced butanol production in Clostridium acetobutylicum. Biotechnol. Bioeng. 2013, 110:887-897.
    • (2013) Biotechnol. Bioeng. , vol.110 , pp. 887-897
    • Mann, M.S.1    Lütke-Eversloh, T.2
  • 34
    • 46149138307 scopus 로고
    • Acetone butanol fermentation by a Clostridium acetobutylicum mutant with high solvent productivity
    • Matta-el-Ammouri G., Janati-Idrissi R., Rambourg J.-M., Petitdemange H., Gay R. Acetone butanol fermentation by a Clostridium acetobutylicum mutant with high solvent productivity. Biomass 1986, 10:109-119.
    • (1986) Biomass , vol.10 , pp. 109-119
    • Matta-el-Ammouri, G.1    Janati-Idrissi, R.2    Rambourg, J.-M.3    Petitdemange, H.4    Gay, R.5
  • 35
    • 0036257268 scopus 로고    scopus 로고
    • A colorimetric assay to quantify dehydrogenase activity in crude cell lysates
    • Mayer K.M., Arnold F.H. A colorimetric assay to quantify dehydrogenase activity in crude cell lysates. J. Biomol. Screen. 2002, 7:135-140.
    • (2002) J. Biomol. Screen. , vol.7 , pp. 135-140
    • Mayer, K.M.1    Arnold, F.H.2
  • 36
    • 84887411422 scopus 로고    scopus 로고
    • Isolation of Clostridium acetobutylicum ATCC824 mutants using propionic and isovaleric acid halogen analogues as suicide substrates
    • Medkor N., Zerdani I., Sattar S. Isolation of Clostridium acetobutylicum ATCC824 mutants using propionic and isovaleric acid halogen analogues as suicide substrates. Int. J. Microbiol. Res. 2010, 1:22-25.
    • (2010) Int. J. Microbiol. Res. , vol.1 , pp. 22-25
    • Medkor, N.1    Zerdani, I.2    Sattar, S.3
  • 37
    • 0020410141 scopus 로고
    • Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium
    • Monot F., Martin J.R., Petitdemange H., Gay R. Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium. Appl. Environ. Microbiol. 1982, 44:1318-1324.
    • (1982) Appl. Environ. Microbiol. , vol.44 , pp. 1318-1324
    • Monot, F.1    Martin, J.R.2    Petitdemange, H.3    Gay, R.4
  • 38
    • 0020604821 scopus 로고
    • Increased ethanol production and tolerance by a pyruvate-negative mutant of Clostridium saccharolyticum
    • Murray W.D., Wemyss K.B., Khan A.W. Increased ethanol production and tolerance by a pyruvate-negative mutant of Clostridium saccharolyticum. Eur. J. Appl. Microbiol. Biotechnol. 1983, 18:71-74.
    • (1983) Eur. J. Appl. Microbiol. Biotechnol. , vol.18 , pp. 71-74
    • Murray, W.D.1    Wemyss, K.B.2    Khan, A.W.3
  • 39
    • 53049086510 scopus 로고    scopus 로고
    • Engineering solventogenic clostridia
    • Papoutsakis E.T. Engineering solventogenic clostridia. Curr. Opin. Biotechnol. 2008, 19:420-429.
    • (2008) Curr. Opin. Biotechnol. , vol.19 , pp. 420-429
    • Papoutsakis, E.T.1
  • 40
    • 30544443489 scopus 로고    scopus 로고
    • A comparative genomic view of clostridial sporulation and physiology
    • Paredes C.J., Alsaker K.V., Papoutsakis E.T. A comparative genomic view of clostridial sporulation and physiology. Nat. Rev. Microbiol. 2005, 3:969-978.
    • (2005) Nat. Rev. Microbiol. , vol.3 , pp. 969-978
    • Paredes, C.J.1    Alsaker, K.V.2    Papoutsakis, E.T.3
  • 41
    • 0000797889 scopus 로고
    • Clostridium acetobutylicum mutants that produce butyraldehyde and altered quantities of solvents
    • Rogers P., Palosaari N. Clostridium acetobutylicum mutants that produce butyraldehyde and altered quantities of solvents. Appl. Environ. Microbiol. 1987, 53:2761-2766.
    • (1987) Appl. Environ. Microbiol. , vol.53 , pp. 2761-2766
    • Rogers, P.1    Palosaari, N.2
  • 42
    • 0022066470 scopus 로고
    • The effect of pH on nitrogen supply, cell lysis, and solvent production in fermentations of Clostridium acetobutylicum
    • Roos J.W., McLaughlin J.K., Papoutsakis E.T. The effect of pH on nitrogen supply, cell lysis, and solvent production in fermentations of Clostridium acetobutylicum. Biotechnol. Bioeng. 1985, 27:681-694.
    • (1985) Biotechnol. Bioeng. , vol.27 , pp. 681-694
    • Roos, J.W.1    McLaughlin, J.K.2    Papoutsakis, E.T.3
  • 43
    • 43049136820 scopus 로고    scopus 로고
    • Combinatorial engineering of microbes for optimizing cellular phenotype
    • Santos C.N.S., Stephanopoulos G. Combinatorial engineering of microbes for optimizing cellular phenotype. Curr. Opin. Chem. Biol. 2008, 12:168-176.
    • (2008) Curr. Opin. Chem. Biol. , vol.12 , pp. 168-176
    • Santos, C.N.S.1    Stephanopoulos, G.2
  • 45
    • 58149268329 scopus 로고    scopus 로고
    • Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
    • Sillers R., Al-Hinai M.A., Papoutsakis E.T. Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations. Biotechnol. Bioeng. 2009, 102:38-49.
    • (2009) Biotechnol. Bioeng. , vol.102 , pp. 38-49
    • Sillers, R.1    Al-Hinai, M.A.2    Papoutsakis, E.T.3
  • 46
    • 57049169148 scopus 로고    scopus 로고
    • Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance
    • Sillers R., Chow A., Tracy B., Papoutsakis E.T. Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance. Metab. Eng. 2008, 10:321-332.
    • (2008) Metab. Eng. , vol.10 , pp. 321-332
    • Sillers, R.1    Chow, A.2    Tracy, B.3    Papoutsakis, E.T.4
  • 48
    • 57449096133 scopus 로고    scopus 로고
    • Development and application of flow-cytometric techniques for analyzing and sorting endospore-forming clostridia
    • Tracy B.P., Gaida S.M., Papoutsakis E.T. Development and application of flow-cytometric techniques for analyzing and sorting endospore-forming clostridia. Appl. Environ. Microbiol. 2008, 74:7497-7506.
    • (2008) Appl. Environ. Microbiol. , vol.74 , pp. 7497-7506
    • Tracy, B.P.1    Gaida, S.M.2    Papoutsakis, E.T.3
  • 49
    • 77950629061 scopus 로고    scopus 로고
    • Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes
    • Tracy B.P., Gaida S.M., Papoutsakis E.T. Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes. Curr. Opin. Biotechnol. 2010, 21:85-99.
    • (2010) Curr. Opin. Biotechnol. , vol.21 , pp. 85-99
    • Tracy, B.P.1    Gaida, S.M.2    Papoutsakis, E.T.3
  • 50
    • 84862010951 scopus 로고    scopus 로고
    • Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications
    • Tracy B.P., Jones S.W., Fast A.G., Indurthi D.C., Papoutsakis E.T. Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications. Curr. Opin. Biotechnol. 2012, 23:364-381.
    • (2012) Curr. Opin. Biotechnol. , vol.23 , pp. 364-381
    • Tracy, B.P.1    Jones, S.W.2    Fast, A.G.3    Indurthi, D.C.4    Papoutsakis, E.T.5
  • 51
    • 0037608873 scopus 로고    scopus 로고
    • Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations
    • Tummala S.B., Junne S.G., Papoutsakis E.T. Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations. J. Bacteriol. 2003, 185:3644-3653.
    • (2003) J. Bacteriol. , vol.185 , pp. 3644-3653
    • Tummala, S.B.1    Junne, S.G.2    Papoutsakis, E.T.3
  • 52
    • 84867579077 scopus 로고    scopus 로고
    • The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum
    • Wietzke M., Bahl H. The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 2012, 96:749-761.
    • (2012) Appl. Microbiol. Biotechnol. , vol.96 , pp. 749-761
    • Wietzke, M.1    Bahl, H.2
  • 53
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • Zhang J.-H., Chung T.D.Y., Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 1999, 4:67-73.
    • (1999) J. Biomol. Screen. , vol.4 , pp. 67-73
    • Zhang, J.-H.1    Chung, T.D.Y.2    Oldenburg, K.R.3


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