Ethyl pyruvate diminishes the inflammatory response to lipopolysaccharide infusion in horses

Equine Veterinary Journal

Volumn 45, Issue 3, 2013, Pages 333-339

  Jacobs, C C ^a   Holcombe, S J ^a   Cook, V L ^a   Gandy, J C ^a   Hauptman, J G ^a   Sordillo, L M ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Cytokines; Endotoxaemia; Ethyl pyruvate; Flunixin meglumine; Horse

Indexed keywords

ACTIN; CLONIXIN; DRUG DERIVATIVE; FLUNIXIN MEGLUMINE; INTERLEUKIN 6; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; NONSTEROID ANTIINFLAMMATORY AGENT; PYRUVIC ACID DERIVATIVE; PYRUVIC ACID ETHYL ESTER; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL; ANIMAL DISEASE; ARTICLE; BODY TEMPERATURE; CHEMICALLY INDUCED DISORDER; DRUG EFFECT; FEMALE; GENE EXPRESSION REGULATION; GENETICS; HORSE; HORSE DISEASE; INFLAMMATION; MALE; METABOLISM;

ACTINS; ANIMALS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; BODY TEMPERATURE; CLONIXIN; FEMALE; GENE EXPRESSION REGULATION; HORSE DISEASES; HORSES; INFLAMMATION; INTERLEUKIN-6; INTERLEUKIN-8; LIPOPOLYSACCHARIDES; MALE; PYRUVATES; TUMOR NECROSIS FACTOR-ALPHA;

EQUIDAE; RODENTIA; SUIDAE;

EID: 84876100966     PISSN: 04251644     EISSN: 20423306     Source Type: Journal    
DOI: 10.1111/j.2042-3306.2012.00634.x     Document Type: Article

References (36)

1. Barton, M.H., Bruce, E.H., Moore, J.N., Norton, N., Anders, B. and Morris, D.D. (1998) Effect of tumor necrosis factor antibody given to horses during early experimentally induced endotoxemia. Am. J. Vet. Res. 59, 792-797.
2. Werners, A.H., Bull, S. and Fink-Gremmels, J. (2005) Endotoxemia: a review with implications for the horse. Equine Vet. J. 37, 371-383.
3. King, J.N. and Gerring, E.L. (1988) Detection of endotoxin in cases of equine colic. Vet. Rec. 123, 269-271.
4. Senior, J.M., Proudman, C.J., Leuwer, M. and Carter, S.D. (2011) Plasma endotoxin in horses presented to an equine referral hospital: correlation to selected clinical parameters and outcomes. Equine Vet. J. 43, 585-591.
5. Barton, M.H. and Collatos, C. (1999) Tumor necrosis factor and interleukin-6 activity and endotoxin concentration in peritoneal fluid and blood of horses with acute abdominal disease. J. Vet. Intern. Med. 13, 457-464.
6. Marshall, J.F. and Blikslager, A.T. (2011) The effect of nonsteroidal anti-inflammatory drugs on the equine intestine. Equine Vet. J. 43, Suppl. 39, 140-144.
7. Jochle, W., Moore, J.N., Brown, J., Baker, G.J., Lowe, J.E., Fubini, S., Reeves, M.J., Watkins, J.P. and White, N.A. (1989) Comparison of detomidine, butorphanol, flunixin meglumine, and xylazine in clinical cases of equine colic. Equine Vet. J., Suppl. 7, 111-116.
8. Cook, V.L., Meyer, C.T., Campbell, N.B. and Blikslager, A.T. (2009) Effect of firocoxib or flunixin meglumine on recovery of ischemic-injured equine jejunum. Am. J. Vet. Res. 70, 992-1000.
9. Fink, M.P. (2003) Ethyl pyruvate: a novel anti-inflammatory agent. Crit. Care Med. 31, Suppl. 1, S51-S56.
10. Han, Y., Englert, J.A., Yang, R., Delude, R.L. and Fink, M.P. (2005) Ethyl pyruvate inhibits nuclear factor-κB-dependent signaling by directly targeting p65. J. Pharmacol. Exp. Ther. 312, 1097-1105.
11. Mizutani, A., Maeda, N., Toku, S., Higa-Nakamine, S., Isohama, Y., Sunakawa, H., Sugahara, K. and Yamamoto, H. (2011) Interaction of ethyl pyruvate in vitro with NF-κB subunits, RelA and p50. Eur. J. Pharmacol. 650, 151-156.
12. Sims, C.A., Wattanasirichaigoon, S. and Menconi, M.J. (2001) Ringer's ethyl pyruvate solution ameliorates ischemia/reperfusion-induced intestinal mucosal injury in rats. Crit. Care Med. 29, 1513-1518.
13. Karabeyoglu, M., Unal, B. and Bozkurt, B. (2008) The effect of ethyl pyruvate on oxidative stress in intestine and bacterial translocation after thermal injury. J. Surg. Res. 144, 59-63.
14. Dong, W., Cai, B., Pena, G., Pisarenko, V., Vida, G., Doucet, D., Lee, M., Sharpe, S., Lu, Q., Xu, D., Ramos, L., Deitch, E.A. and Ulloa, L. (2010) Ethyl pyruvate prevents inflammatory responses and organ damage in porcine hemorrhage. Shock 34, 205-213.
15. Schroeder, E.L., Holcombe, S.J., Cook, V.L., James, M.D., Gandy, J.C., Hauptman, J.G. and Sordillo, L.M. (2011) Preliminary safety and biological efficacy studies of ethyl pyruvate in normal mature horses. Equine Vet. J. 43, 341-347.
16. Cook, V.L., Holcombe, S.J., Gandy, J.C., Corl, C.M. and Sordillo, L.M. (2011) Ethyl pyruvate decreases pro-inflammatory gene expression in lipopolysaccharide-stimulated equine monocytes. Vet. Immunol. Immunopathol. 141, 92-99.
17. Baskett, A., Barton, M.H., Norton, N., Anders, B. and Moore, J.N. (1997) Effect of pentoxifylline, flunixin meglumine, and their combination on a model of endotoxemia in horses. Am. J. Vet. Res. 58, 1291-1299.
18. Nieto, J.E., MacDonald, M.H., Poulin Braim, A.E. and Aleman, M. (2009) Effect of lipopolysaccharide infusion on gene expression of inflammatory cytokines in normal horses in vivo. Equine Vet. J. 41, 717-719.
19. Pritchett, L.C., Ulibarri, C.U., Roberts, M.C., Schneider, R.K. and Sellon, D.C. (2003) Identification of potential physiological and behavioral indicators of postoperative pain in horses after exploratory celiotomy for colic. Appl. Anim. Behav. Sci. 80, 31-43.
20. Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408.
21. MacKay, R.J., Merritt, A.M., Zertuche, J.M., Whittington, M. and Skelley, L.A. (1991) Tumor necrosis factor activity in the circulation of horses given endotoxin. Am. J. Vet. Res. 52, 533-542.
22. Ewert, K.M., Fessler, J.F., Templeton, C.B., Bottoms, G.D., Latshaw, H.S. and Johnson, M.A. (1985) Endotoxin-induced hematologic and blood chemical changes in ponies: effects of flunixin meglumine, dexamethasone, and prednisolone. Am. J. Vet. Res. 46, 24-29.
23. Kao, K.K. and Fink, M.P. (2010) The biochemical basis for the anti-inflammatory and cytoprotective actions of ethyl pyruvate and related compound. Biochem. Pharmacol. 80, 151-159.
24. Sivapalaratnam, S., Farrugia, R., Nieuwdorp, M., Langford, C.F., van Beem, R.T., Maiwald, S., Zwaginga, J.J., Gusnanto, A., Watkins, N.A., Trip, M.D. and Ouwehand, W.H. (2011) Identification of candidate genes linking systemic inflammation to atherosclerosis; results of a human in vivo LPS infusion study. BMC Med. Genomics 4, 64-68.
25. Moore, J.N., Hardee, M.M. and Hardee, G.E. (1986) Modulation of arachidonic acid metabolism in endotoxic horses: comparison of flunixin meglumine, phenylbutazone, and a selective thromboxane synthetase inhibitor. Am. J. Vet. Res. 47, 110-113.
26. Jackman, B.R., Moore, J.N., Barton, M.H. and Morris, D.D. (1994) Comparison of the effects of ketoprofen and flunixin meglumine on the in vitro responses of equine peripheral blood monocytes to bacterial endotoxin. Can. J. Vet. Res. 58, 138-143.
27. Harada, T., Moore, B.A., Yang, R., Cruz, R.J., Delude, R.L. and Fink, M.P. (2005) Ethyl pyruvate ameliorates ileus induced by bowel manipulation in mice. Surgery 138, 530-537.
28. Natale, L., Piepoli, A.L. and De Salvia, M.A. (2003) Interleukins 1 beta and 6 induce functional alteration of rat colonic motility: an in vitro study. Eur. J. Clin. Invest. 33, 704-712.
29. Chen, H., Bai, H., Xi, M., Liu, R., Qin, X., Liang, X., Zhang, W., Zhang, X., Li, W. and Hai, C. (2011) Ethyl pyruvate protects rats from phosgene-induced pulmonary edema by inhibiting cyclooxygenase2 and inducible nitric oxide synthase expression. J. Appl. Toxicol. DOI 10.1002/jat.1713.
30. Ulloa, L., Ochani, M., Yang, H., Tanovic, M., Halperin, D., Yang, R., Czura, C.J., Fink, M.P. and Tracey, K.J. (2002) Ethyl pyruvate prevents lethality in mice with established lethal sepsis and systemic inflammation. Proc. Natl Acad. Sci. U.S.A. 99, 12351-12356.
31. Yu, D.H., Noh, D.H., Song, R.H. and Park, J. (2010) Ethyl pyruvate downregulates tumor necrosis factor alpha and interleukin (IL)-6 and upregulates IL-10 in lipopolysaccharide-stimulated canine peripheral blood mononuclear cells. J. Vet. Med. Sci. 72, 1379-1381.
32. Yazar, E., Er, A., Uney, K., Altunok, V. and Elmas, M. (2007) Effect of flunixin meglumine on cytokine levels in experimental endotoxemia in mice. J. Vet. Med. 54, 352-355.
33. Bryant, C.E., Farnfield, B.A. and Janicke, H.J. (2003) Evaluation of the ability of carprofen and flunixin meglumine to inhibit activation of nuclear factor kappa B. Am. J. Vet. Res. 64, 211-215.
34. Venkataraman, R., Kellum, J.A., Song, M. and Fink, M.P. (2002) Resuscitation with Ringer's ethyl pyruvate solution prolongs survival and modulates plasma cytokine and nitrite/nitrate concentrations in a rate model of lipolysaccharide-induced shock. Shock 18, 507-512.
35. Andaluz-Ojeda, D., Bobillo, F., Iglesias, V., Almansa, R., Rico, L., Gandia, F., Resino, S., Tamayo, E., Ortiz de Lejarazu, R. and Bermejo-Martin, J.F. (2012) A combined score of pro- and anti-inflammatory prediction in sever sepsis. Cytokine 57, 332-336.
36. Remick, D.G., Bolgos, G.R., Siddiqui, J., Shin, J. and Nemzek, J.A. (2002) Six at six: interleukin-6 measured 6 hours after the initiation of sepsis predicts mortality over 3 days. Shock 17, 463-467.