Quantum dot imaging platform for single-cell molecular profiling

Nature Communications

Volumn 4, Issue , 2013, Pages

  Zrazhevskiy, Pavel ^a   Gao, Xiaohu ^a  

^a University of Washington   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PROTEIN A; QUANTUM DOT;

ANTIBODY; FLUORESCENCE; GENE EXPRESSION; MOLECULAR ANALYSIS; PHYSIOLOGICAL RESPONSE; PROTEIN; QUANTUM MECHANICS; SIGNAL; STOICHIOMETRY;

ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; CELL FUNCTION; CONTROLLED STUDY; COVALENT BOND; FLUORESCENCE IMAGING; GENE EXPRESSION; HUMAN; HUMAN CELL; IMAGING SYSTEM; MOLECULAR CLONING; MOLECULAR DIAGNOSIS; MOLECULAR PROBE; OPTICAL RESOLUTION; PATHOGENESIS; PROTEIN AGGREGATION; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; SINGLE CELL MOLECULAR PROFILING; STAINING; STOICHIOMETRY; SYSTEMS BIOLOGY;

FLUORESCENT DYES; HELA CELLS; HUMANS; QUANTUM DOTS; SINGLE-CELL ANALYSIS;

EID: 84875902633     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms2635     Document Type: Article

References (50)

1. de Souza, N. Single-cell methods. Nat. Methods 9, 35 (2012).
2. Liotta, L. & Petricoin, E. Molecular profiling of human cancer. Nat. Rev. Genet. 1, 48-56 (2000).
3. Michalet, X. et al. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307, 538-544 (2005). (Pubitemid 40165671)
4. Zhang, J., Campbell, R. E., Ting, A. Y. & Tsien, R. Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002). (Pubitemid 35477369)
5. Zrazhevskiy, P. & Gao, X. Multifunctional quantum dots for personalized medicine. Nano Today 4, 414-428 (2009).
6. Gleason, D. F. & Mellinger, G. T. Prediction of prognosis for prostatic adenocarcinoma by combined histological grading and clinical staging. J. Urol. 111, 58-64 (1974).
7. Liotta, L. A. & Kohn, E. C. The microenvironment of the tumour-host interface. Nature 411, 375-379 (2001).
8. Cornett, D. S., Reyzer, M. L., Chaurand, P. & Caprioli, R. M. MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat. Meth. 4, 828-833 (2007). (Pubitemid 350055580)
9. Stoeckli, M., Chaurand, P., Hallahan, D. E. & Caprioli, R. M. Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat. Med. 7, 493-496 (2001). (Pubitemid 32298555)
10. Bandura, D. R. et al. Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry. Anal. Chem. 81, 6813-6822 (2009).
11. Bendall, S. C. et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687-696 (2011).
12. Ornatsky, O., Baranov, V. I., Bandura, D. R., Tanner, S. D. & Dick, J. Multiple cellular antigen detection by ICP-MS. J. Immunol. Methods 308, 68-76 (2006). (Pubitemid 43107955)
13. Cao, Y. C., Jin, R. C., Nam, J. M., Thaxton, C. S. & Mirkin, C. A. Raman dyelabeled nanoparticle probes for proteins. J. Am. Chem. Soc. 125, 14676-14677 (2003). (Pubitemid 37475538)
14. Cao, Y. W. C., Jin, R. C. & Mirkin, C. A. Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. Science 297, 1536-1540 (2002). (Pubitemid 34970997)
15. Liu, Z. et al. Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube Raman tags in the near-infrared. Nano Res. 3, 222-233 (2010).
16. Zavaleta, C. L. et al. Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy. Proc. Natl Acad. Sci. USA 106, 13511-13516 (2009).
17. Glass, G., Papin, J. A. & Mandell, J. W. SIMPLE: a sequential immunoperoxidase labeling and erasing method. J. Histochem. Cytochem. 57, 899-905 (2009).
18. Pirici, D. et al. Antibody elution method for multiple immunohistochemistry on primary antibodies raised in the same species and of the same subtype. J. Histochem. Cytochem. 57, 567-575 (2009).
19. Toth, Z. E. & Mezey, E. Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species. J. Histochem. Cytochem. 55, 545-554 (2007). (Pubitemid 46791937)
20. Wahlby, C., Erlandsson, F., Bengtsson, E. & Zetterberg, A. Sequential immunofluorescence staining and image analysis for detection of large numbers of antigens in individual cell nuclei. Cytometry 47, 32-41 (2002). (Pubitemid 34032577)
21. Micheva, K. D., Busse, B., Weiler, N. C., O'Rourke, N. & Smith, S. J. Singlesynapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68, 639-653 (2010).
22. Schubert, W. et al. Analyzing proteome topology and function by automated multidimensional fluorescence microscopy. Nat. Biotechnol. 24, 1270-1278 (2006). (Pubitemid 44564774)
23. Bruchez, Jr M., Moronne, M., Gin, P., Weiss, S. & Alivisatos, A. P. Semiconductor nanocrystals as fluorescent biological labels. Science 281, 2013-2016 (1998). (Pubitemid 28475485)
24. Chan, W. C. & Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281, 2016-2018 (1998). (Pubitemid 28475486)
25. Dubertret, B. et al. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298, 1759-1762 (2002). (Pubitemid 35404113)
26. Gao, X., Cui, Y., Levenson, R. M., Chung, L. W. & Nie, S. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat. Biotechnol. 22, 969-976 (2004). (Pubitemid 39014473)
27. Pellegrino, T. et al. Hydrophobic nanocrystals coated with an amphiphilic polymer shell: a general route to water soluble nanocrystals. Nano Lett. 4, 703-707 (2004). (Pubitemid 38586637)
28. Wu, X. Y. et al. Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat. Biotechnol. 21, 41-46 (2003).
29. Zrazhevskiy, P., Sena, M. & Gao, X. H. Designing multifunctional quantum dots for bioimaging, detection, and drug delivery. Chem. Soc. Rev. 39, 4326-4354 (2010).
30. Fountaine, T. J., Wincovitch, S. M., Geho, D. H., Garfield, S. H. & Pittaluga, S. Multispectral imaging of clinically relevant cellular targets in tonsil and lymphoid tissue using semiconductor quantum dots. Mod. Pathol. 19, 1181-1191 (2006). (Pubitemid 44268188)
31. Giepmans, B. N., Deerinck, T. J., Smarr, B. L., Jones, Y. Z. & Ellisman, M. H. Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nat. Meth. 2, 743-749 (2005). (Pubitemid 41486205)
32. Levenson, R. M. & Mansfield, J. R. Multispectral imaging in biology and medicine: slices of life. Cytometry A 69A, 748-758 (2006). (Pubitemid 44469987)
33. Liu, J., Lau, S. K., Varma, V. A., Kairdolf, B. A. & Nie, S. Multiplexed detection and characterization of rare tumor cells in Hodgkin's lymphoma with multicolor quantum dots. Anal. Chem. 82, 6237-6243 (2010).
34. Liu, J. et al. Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots. ACS Nano 4, 2755-2765 (2010).
35. Yezhelyev, M. V. et al. In situ molecular profiling of breast cancer biomarkers with multicolor quantum dots. Adv. Mater. 19, 3146-3151 (2007). (Pubitemid 350044451)
36. Xing, Y. et al. Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. Nat. Protoc. 2, 1152-1165 (2007). (Pubitemid 46901294)
37. Chen, S. F., Zheng, J., Li, L. Y. & Jiang, S. Y. Strong resistance of phosphorylcholine self-assembled monolayers to protein adsorption: insights into nonfouling properties of zwitterionic materials. J. Am. Chem. Soc. 127, 14473-14478 (2005). (Pubitemid 41457607)
38. Moks, T. et al. Staphylococcal protein A consists of five IgG-binding domains. Eur. J. Biochem. 156, 637-643 (1986).
39. Atkins, K. L. et al. S. aureus IgG-binding proteins SpA and Sbi: host specificity and mechanisms of immune complex formation. Mol. Immunol. 45, 1600-1611 (2008). (Pubitemid 351227405)
40. Hanson, D. C., Phillips, M. L. & Schumaker, V. N. Electron microscopic and hydrodynamic studies of protein A-immunoglobulin G soluble complexes. J. Immunol. 132, 1386-1396 (1984). (Pubitemid 14154567)
41. Hanson, D. C. & Schumaker, V. N. A model for the formation and interconversion of protein A-immunoglobulin G soluble complexes. J. Immunol. 132, 1397-1409 (1984). (Pubitemid 14154568)
42. Mota, G., Ghetie, V. & Sjoquist, J. Characterization of the soluble complex formed by reacting rabbit IgG with protein A of S. aureus. Immunochemistry 15, 639-642 (1978). (Pubitemid 9053130)
43. Myhre, E. B. & Kronvall, G. Immunochemical aspects of fc-mediated binding of human-Igg subclasses to Group-a, Group-C and Group-G Streptococci. Mol. Immunol. 17, 1563-1573 (1980). (Pubitemid 11221144)
44. Ghazani, A. A. et al. High throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals. Nano Lett. 6, 2881-2886 (2006). (Pubitemid 46129588)
45. Huang, D. H. et al. Comparison and optimization of multiplexed quantum dotbased immunohistofluorescence. Nano Res. 3, 61-68 (2010).
46. Goldman, E. R. et al. Avidin: a natural bridge for quantum dot-antibody conjugates. J. Am. Chem. Soc. 124, 6378-6382 (2002). (Pubitemid 34579391)
47. Jaiswal, J. K., Mattoussi, H., Mauro, J. M. & Simon, S. M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 21, 47-51 (2003). (Pubitemid 36055826)
48. Jin, T. et al. Antibody-protein A conjugated quantum dots for multiplexed imaging of surface receptors in living cells. Mol. BioSyst. 6, 2325-2331 (2010).
49. Mattoussi, H. et al. Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered recombinant protein. J. Am. Chem. Soc. 122, 12142-12150 (2000). (Pubitemid 32062250)
50. Zhong, X., Feng, Y., Knoll, W. & Han, M. Alloyed ZnxCd1-xS nanocrystals with highly narrow luminescence spectral width. J. Am. Chem. Soc. 125, 13559-13563 (2003). (Pubitemid 37352138)