Fission yeast TORC1 prevents eIF2α phosphorylation in response to nitrogen and amino acids via gcn2 kinase

Journal of Cell Science

Volumn 125, Issue 24, 2012, Pages 5955-5959

  Valbuena, Noelia ^a   Rozalén, Ana Elisa ^a   Moreno, Sergio ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

eIF2 ; Gcn2; Rapamycin; S. pombe; TOR

Indexed keywords

AMINO ACID; AMMONIUM CHLORIDE; INITIATION FACTOR 2ALPHA; NITROGEN; PROTEIN SERINE THREONINE KINASE; RAPAMYCIN; SERINE; TARGET OF RAPAMYCIN KINASE; TARGET OF THE RAPAMYCIN 2 PROTEIN; TORC1 PROTEIN; UNCLASSIFIED DRUG; MECHANISTIC TARGET OF RAPAMYCIN COMPLEX 1; MULTIPROTEIN COMPLEX; PROTEIN KINASE R;

ARTICLE; CELL VACUOLE; FISSION YEAST; FUNGUS GROWTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; SCHIZOSACCHAROMYCES POMBE; SIGNAL TRANSDUCTION; TRANSLATION INITIATION; YEAST; YEAST CELL; ENZYMOLOGY; GENETICS; METABOLISM; PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES;

EUKARYOTA; SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

AMINO ACIDS; EIF-2 KINASE; MULTIPROTEIN COMPLEXES; NITROGEN; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SIROLIMUS; TOR SERINE-THREONINE KINASES;

EID: 84875610650     PISSN: 00219533     EISSN: 14779137     Source Type: Journal    
DOI: 10.1242/jcs.105395     Document Type: Article

References (33)

1. Alvarez, B. and Moreno, S. (2006). Fission yeast Tor2 promotes cell growth and represses cell differentiation. J. Cell Sci. 119, 4475-4485.
2. Bähler, J., Wu, J. Q., Longtine, M. S., Shah, N. G., McKenzie, A., 3rd, Steever, A. B., Wach, A., Philippsen, P. and Pringle, J. R. (1998). Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14, 943-951.
3. Berlanga, J. J., Rivero, D., Martín, R., Herrero, S., Moreno, S. and de Haro, C. (2010). Role of mitogen-activated protein kinase Sty1 in regulation of eukaryotic initiation factor 2alpha kinases in response to environmental stress in Schizosaccharomyces pombe. Eukaryot. Cell 9, 194-207.
4. Blommaart, E. F., Luiken, J. J., Blommaart, P. J., van Woerkom, G. M. and Meijer, A. J. (1995). Phosphorylation of ribosomal protein S6 is inhibitory for autophagy in isolated rat hepatocytes. J. Biol. Chem. 270, 2320-2326.
5. Cherkasova, V. A. and Hinnebusch, A. G. (2003). Translational control by TOR and TAP42 through dephosphorylation of eIF2alpha kinase GCN2. Genes Dev. 17, 859-872.
6. Dever, T. E. (2002). Gene-specific regulation by general translation factors. Cell 108, 545-556.
7. Fingar, D. C., Salama, S., Tsou, C., Harlow, E. and Blenis, J. (2002). Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E. Genes Dev. 16, 1472-1487.
8. Foiani, M., Marini, F., Gamba, D., Lucchini, G. and Plevani, P. (1994). The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication. Mol. Cell. Biol. 14, 923-933.
9. Hartmuth, S. and Petersen, J. (2009). Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress. J. Cell Sci. 122, 1737-1746.
10. Hay, N. and Sonenberg, N. (2004). Upstream and downstream of mTOR. Genes Dev. 18, 1926-1945.
11. Hayashi, T., Hatanaka, M., Nagao, K., Nakaseko, Y., Kanoh, J., Kokubu, A., Ebe, M. and Yanagida, M. (2007). Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits. Genes Cells 12, 1357-1370.
12. Hinnebusch, A. G. (2005). Translational regulation of GCN4 and the general amino acid control of yeast. Annu. Rev. Microbiol. 59, 407-450.
13. Jacinto, E., Loewith, R., Schmidt, A., Lin, S., Rüegg, M. A., Hall, A. and Hall, M. N. (2004). Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat. Cell Biol. 6, 1122-1128.
14. Kubota, H., Obata, T., Ota, K., Sasaki, T. and Ito, T. (2003). Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2 a kinase GCN2. J. Biol. Chem. 278, 20457-20460.
15. Loewith, R., Jacinto, E., Wullschleger, S., Lorberg, A., Crespo, J. L., Bonenfant, D., Oppliger, W., Jenoe, P. and Hall, M. N. (2002). Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol. Cell 10, 457-468.
16. Matsuo, T., Otsubo, Y., Urano, J., Tamanoi, F. and Yamamoto, M. (2007). Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast. Mol. Cell. Biol. 27, 3154-3164.
17. Moreno, S., Klar, A. and Nurse, P. (1991). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823.
18. Nakashima, A., Sato, T. and Tamanoi, F. (2010). Fission yeast TORC1 regulates phosphorylation of ribosomal S6 proteins in response to nutrients and its activity is inhibited by rapamycin. J. Cell Sci. 123, 777-786.
19. Narasimhan, J., Staschke, K. A. and Wek, R. C. (2004). Dimerization is required for activation of eIF2 kinase Gcn2 in response to diverse environmental stress conditions. J. Biol. Chem. 279, 22820-22832.
20. Noda, T. and Ohsumi, Y. (1998). Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast. J. Biol. Chem. 273, 3963-3966.
21. Petersen, J. and Nurse, P. (2007). TOR signalling regulates mitotic commitment through the stress MAP kinase pathway and the Polo and Cdc2 kinases. Nat. Cell Biol. 9, 1263-1272.
22. Proud, C. G. (2005). eIF2 and the control of cell physiology. Semin. Cell Dev. Biol. 16, 3-12.
23. Sarbassov, D. D., Ali, S. M., Kim, D. H., Guertin, D. A., Latek, R. R., Erdjument-Bromage, H., Tempst, P. and Sabatini, D. M. (2004). Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr. Biol. 14, 1296-1302.
24. Sarbassov, D. D., Guertin, D. A., Ali, S. M. and Sabatini, D. M. (2005). Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307, 1098-1101.
25. Shigemitsu, K., Tsujishita, Y., Hara, K., Nanahoshi, M., Avruch, J. and Yonezawa, K. (1999). Regulation of translational effectors by amino acid and mammalian target of rapamycin signaling pathways. Possible involvement of autophagy in cultured hepatoma cells. J. Biol. Chem. 274, 1058-1065.
26. Uritani, M., Hidaka, H., Hotta, Y., Ueno, M., Ushimaru, T. and Toda, T. (2006). Fission yeast Tor2 links nitrogen signals to cell proliferation and acts downstream of the Rheb GTPase. Genes Cells 11, 1367-1379.
27. Valbuena, N., Guan, K.-L. and Moreno, S. (2012). The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast. J. Cell Sci. 125, 1920-1928.
28. Valenzuela, L., Aranda, C. and González, A. (2001). TOR modulates GCN4-dependent expression of genes turned on by nitrogen limitation. J. Bacteriol. 183, 2331-2334.
29. Weisman, R., Roitburg, I., Schonbrun, M., Harari, R. and Kupiec, M. (2007). Opposite effects of tor1 and tor2 on nitrogen starvation responses in fission yeast. Genetics 175, 1153-1162.
30. Yan, G., Shen, X. and Jiang, Y. (2006). Rapamycin activates Tap42-associated phosphatases by abrogating their association with Tor complex 1. EMBO J. 25, 3546-3555.
31. Yan, G., Lai, Y. and Jiang, Y. (2012). The TOR complex 1 is a direct target of Rho1 GTPase. Mol. Cell 45, 743-753.
32. Yang, R., Wek, S. A. and Wek, R. C. (2000). Glucose limitation induces GCN4 translation by activation of Gcn2 protein kinase. Mol. Cell. Biol. 20, 2706-2717.
33. Zhan, K., Narasimhan, J. and Wek, R. C. (2004). Differential activation of eIF2 kinases in response to cellular stresses in Schizosaccharomyces pombe. Genetics 168, 1867-1875.