Relative expression of cholesterol transport-related proteins and inflammation markers through the induction of 7-ketosterol-mediated stress in Caco-2 cells

Food and Chemical Toxicology

Volumn 56, Issue , 2013, Pages 247-253

  Alemany, L ^a   Laparra, J M ^b   Barbera R ^a   Alegria A ^a  

^a UNIVERSITY OF VALENCIA   (Spain)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

Author keywords

7 Ketocholesterol; 7 Ketostigmasterol; Cholesterol metabolism; Inflammation; Oxysterols

Indexed keywords

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; 7 KETOSTIGMASTEROL; 7 OXOCHOLESTEROL; ABC TRANSPORTER G5; ABC TRANSPORTER G8; BIOLOGICAL MARKER; BRADYKININ; CALCIUM; CARRIER PROTEINS AND BINDING PROTEINS; CHOLESTEROL ACYLTRANSFERASE; INTERLEUKIN 10; INTERLEUKIN 1BETA; INTERLEUKIN 8; MESSENGER RNA; NIEMANN PICK C1 LIKE 1 PROTEIN; STEROL DERIVATIVE; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM CELL LEVEL; CELL STRAIN CACO 2; CELL STRESS; CHOLESTEROL METABOLISM; CONTROLLED STUDY; CYTOTOXICITY; ENERGY METABOLISM; EXCRETION; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; INFLAMMATION; INTERNALIZATION; MITOCHONDRIAL MEMBRANE POTENTIAL; PROTEIN EXPRESSION;

ACETYL-COA C-ACETYLTRANSFERASE; ACYL COENZYME A; ANTICHOLESTEREMIC AGENTS; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL MARKERS; BIOLOGICAL TRANSPORT; BRADYKININ; CACO-2 CELLS; DOWN-REGULATION; HUMANS; INFLAMMATION; INTERLEUKIN-10; INTERLEUKIN-1BETA; INTERLEUKIN-8; KETOCHOLESTEROLS; LIPOPROTEINS; MEMBRANE PROTEINS; RNA, MESSENGER; STIGMASTEROL; TUMOR NECROSIS FACTOR-ALPHA; UP-REGULATION;

EID: 84875286005     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2013.02.040     Document Type: Article

References (34)

1. Alemany L., Laparra J.M., Barberá R., Alegría A. Evaluation of the cytotoxic effect of 7keto-cholesterol in human intestinal (Caco-2) cells. Food Chem. Toxicol. 2012, 50:3106-3113.
2. Brown A.J., Jessup W. Oxysterols: sources, cellular storage and metabolism, and new insights into their roles in cholesterol homeostasis. Mol. Aspects Med. 2009, 30:111-122.
3. Chen J., Li Q., Zhang Y., Yang P., Zong Y., Qu S., Liu Z. Oleic acid decreases the expression of a cholesterol transport-related protein (NPC1L1) by the induction of endoplasmic reticulum stress in Caco-2 cells. J. Physiol. Biochem. 2011, 67:153-163.
4. 2-iduced oxidative stress in Caco-2 cells. Food Chem. 2008, 106:1180-1187.
5. Davis H.R., Zhu L.J., Hoos L.M., Tetzloff G., Maguire M., Liu J., Yao X., Iyer S.P.N., Lam M.-H., Lund E.G., Detmers P.A., Graziano M.P., Altmann S.W. Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. J. Biol. Chem. 2004, 279:33586-33592.
6. De Smet E., Mensink R.P., Plat J. Effects of plant sterols and stanols on intestinal cholesterol metabolism: suggested mechanisms from past to present. Mol. Nutr. Food Res. 2012, 56:1058-1072.
7. García-Llatas G., Rodríguez-Estrada M.T. Current and new insights on phytosterol oxides in plant sterol-enriched food. Chem. Phys. Lipids 2011, 164:607-624.
8. Hui D.Y., Howles P.N. Molecular mechanism of cholesterol absorption and transport in the intestine. Cell Dev. Biol. 2005, 16:183-192.
9. Koschutnig K., Heikkinen S., Kemmo S., Lampi A.M., Piironen V., Wagner K.H. Cytotoxic and apoptotic effects of single and mixed oxides of β-sitosterol on HepG2-cells. Toxicol in Vitro 2009, 23:755-762.
10. Laparra Llopis J.M., Sanz Herranz Y. Gliadins induce TNFα production through cAMP-dependent protein kinase A activation in intestinal cells (Caco-2). J. Physiol. Biochem. 2010, 66:153-159.
11. Lemaire S., Lizard G., Monier S., Miguet C., Gueldry S., Volot F., Gambert P., Néel D. Different patterns of IL-1β secretion, adhesion molecule expression and apoptosis induction in human endothelial cells treated with 7α-, 7β-hydroxycholesterol, or 7-ketocholesterol. FEBS Lett. 1998, 440:434-439.
12. Lemaire-Ewing S., Prunet C., Montagne T., Vejux A., Berthier A., Bessède L., Corcos L., Gambert P., Néel D., Lizard G. Comparison of the cytotoxic, pro-oxidant and pro-inflammatory characteristics of different oxysterols. Cell Biol. Toxicol. 2005, 21:97-114.
13. Leonarduzzi G., Sevanian A., Sottero B., Arkan M.C., Biasi F., Chiarpotto E., Basaga H., Poli G. Up-regulation of the fibrogenic cytokine TGF-beta1 by oxysterols: a mechanistic link between cholesterol and aterosclerosis. Faseb J. 2001, 15:1619-1621.
14. Liang Y.T., Wong W.T., Guan L., Tian X.Y., Ma K.Y., Huang Y., Chen Z. Effect of phytosterols and their oxidation products on lipoprotein profiles and vascular function in hamster fed a high cholesterol diet. Atherosclerosis 2011, 219:124-133.
15. Lin D.S., Steiner R.D., Merkens L.S., Pappu A.S., Connor W.E. The effects of sterol structure upon sterol esterification. Atherosclerosis 2010, 208:155-160.
16. Livak K.J., Schmittge T.D. Analysis of relative gene expression data using Real-Time Quantitative PCR and the 2T-ΔΔC method. Methods 2001, 25:402-408.
17. Lizard G., Lemaire S., Monier S., Gueldry S., Néel D., Gambert P. Induction of apoptosis and of interleukin-1β secretion by 7β-hydroxycholesterol and 7-ketocholesterol: partial inhibition by Bcl-2 overexpression. FEBS Lett. 1997, 419:276-280.
18. Lordan S., Mackrill J.J., O'Brien N.M. Oxysterols and mechanisms of apoptotic signaling: implications in the pathology of degenerative diseases. J. Nutr. Biochem. 2009, 20:321-336.
19. Mascia C., Maina M., Chiarpotto E., Leonarduzzi G., Poli G., Biasi F. Proinflammatory effect of cholesterol and its oxidation products on Caco-2 human enterocyte-like cells: effective protection by epigallocatechin-3-gallate. Free Radical Biol. Med. 2010, 49:2049-2057.
20. Otaegui-Arrazola A., Menendez-Carreño M., Ansorena D. Oxysterols: a world to explore. Food Chem. Toxicol. 2010, 48:3289-3303.
21. Othman R.A., Moghadasian M.H. Beyond cholesterol-lowering effects of plant sterols: clinical and experimental evidence of anti-inflammatory properties. Nutr. Rev. 2011, 69:371-382.
22. Plat J., Mensink R.P. Plant stanol and sterol esters in the control of blood cholesterol levels: mechanism and safety aspects. Am. J. Cardiol. 2005, 96:15D-22D.
23. Prunet C., Montagne T., Véjux A., Laubriet A., Rohmer J.-F., Riedinger J.-M., Athias A., Lemaire-Ewing S., Néel D., Petit J.-M., Steinmetz E., Brenot R., Gambert P., Lizard G. Multiplesed flow cytometric analyses of pro- and anti-inflammatoy cytokines in the culture media of oxysterol-treated human monocytic cells and in the sera of atherosclerotic patients. ISAC 2006, 69A:359-373.
24. Roussi S., Winter A., Gosse F., Werner D., Zhang X., Marchioni E., Geoffroy P., Miesch M., Raul F. Different apoptotic mechanisms are involved in the antiproliferative effects of 7β-hydroxysitosterol and 7β-hydroxycholesterol in human colon cancer cells. Cell Death Differ. 2005, 12:128-135.
25. Roussi S., Gossé F., Aoudé-Werner D., Zhang X., Marchioni E., Geoffroy P., Miesch M., Raul F. Mitochondrial perturbation, oxidative stress and lysosomal destabilization are involved in 7β-hydroxysitosterol and 7β-hydroxycholesterol triggered apoptosis in human colon cancer cells. Apoptosis 2007, 12:87-96.
26. Ryan E., Chopra J., McCarthy F., Maguire A.R., O'Brien N.M. Qualitative and quantitative comparison of the cytotoxity and apoptotic potential of phytosterol oxidation products with their corresponding cholesterol oxidation products. Brit. J. Nutr. 2005, 94:443-451.
27. Ryan E., McCarthy F., Maguire A.R., O'Brien N.M. Phytosterol oxidation products: their formation, occurrence, and biological effects. Food Rev. Int. 2009, 25:157-174.
28. Sato Y., Suzuki R., Kobayashi M., Itagaki S., Hirano T., Noda T., Mizuno S., Sugawara M., Iseki K. Involvement of cholesterol membrane transporter Niemann-Pick C1-Like 1 in the intestinal absorption of lutein. J. Pharm. Pharmaceut. Sci. 2012, 15:256-264.
29. Song H.-L., Lu S., Ma L., Li Y., Liu P. Effect of TNF-α on tight junctions between the epithelial cells of intestinal mucosal barrier. World Chin. J. Digestol. 2004, 12:1303-1306.
30. Stryer, L.,1988. Bioquímica, third ed. Barcelona, Spain.
31. Sudhop T., Lütjohann D., von Bergmann K. Sterol transporters: targets of natural sterols and new lipid lowering drugs. Pharmacol. Therapeut. 2005, 105:333-341.
32. Temel R.E., Gebre A.K., Parks J.S., Rudel L.L. Compared with Acyl-CoA: cholesterol O-acyltransferase (ACAT) and lecithin:cholesterol acyltransferase, ACAT2 displays the greatest capacity to differentiate cholesterol from sitosterol. J. Biol. Chem. 2003, 278:47594-47601.
33. Vejux A., Montagne T., Martine L., Zarrouk A., Riedinger J.-M., Lizard G. Absence of oxysterol-like side effects in human monocytic cells treated with phytosterol and oxyphytosterols. J. Agric. Food Chem. 2012, 60:4060-4066.
34. Zhang J.H., Ge L., Qi W., Zhang L., Miao H.-H., Li B.-L., Yang M., Song B.-L. The N-terminal domain of NPC1L1 protein binds cholesterol and plays essential roles in cholesterol uptake. J. Biol. Chem. 2011, 286:25088-25097.