Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy

Bioimaging

Volumn 4, Issue 3, 1996, Pages 187-197

  Kano, Hiroshi ^a   Van Der Voort, Hans T M ^b   Schrader, Martin ^a   Van Kempen, Geert M P ^c   Hell, Stefan W ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b Scientific Volume Imaging BV   (Netherlands)

^c DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

avalanche photodiode; F actin; fluorescence microscopy; high sensitivity; mouse fibroblast; object scanning; point spread function; resolution; restoration; two photon excitation

Indexed keywords

ANIMAL CELL; ARTICLE; FIBROBLAST; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA;

EID: 0030511985     PISSN: 09669051     EISSN: None     Source Type: Journal    
DOI: 10.1002/1361-6374(199609)4:3<187::AID-BIO9>3.3.CO;2-V     Document Type: Article

References (8)

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2. Hell S W, Bahlmann K, Schrader M, Soini A, Malak H, Gryczynski I and Lakowicz J R 1996 Three-photon excitation in fluorescence microscopy J. Biomed. Opt. 1 71-3
3. Denk W 1996 Two-photon excitation in functional biological imaging J. Biomed. Opt. 1 296-304
4. Hell S and Stelzer E H K 1992 Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation Opt. Commun. 93 277-82
5. Gu M and Sheppard C J R 1995 Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy J. Microsc. 177 128-37
6. Pawley J (ed) 1995 Handbook of Biological Confocal Microscopy (New York: Plenum)
7. Goodman J W 1968 Introduction to Fourier Optics (New York: McGraw Hill)
8. Holmes T J, Bhattacharyya S, Cooper J A, Hanzel D, Krishnamurthi V, Lin W, Roysam B, Szarowski D H and Turner J N 1995 Light microscopic images reconstruction by maximum likelihood deconvolution Handbook of Biological Confocal Microscopy ed J Pawley (New York: Plenum)