One-step production of α-ketoglutaric acid from glutamic acid with an engineered l-amino acid deaminase from Proteus mirabilis

Journal of Biotechnology

Volumn 164, Issue 1, 2013, Pages 97-104

  Liu, Long ^a   Hossain, Gazi Sakir ^a   Shin, Hyun Dong ^b   Li, Jianghua ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

Ketogluteric acid; Glutamic acid; L Amino acid deaminase; Proteus mirabilis

Indexed keywords

BIOCHEMICAL PROPERTIES; CHEMICAL SYNTHESIS; ENVIRONMENTAL POLLUTIONS; EXPRESSION LEVELS; GLUTAMIC ACID; L-AMINO ACIDS; PROTEUS MIRABILIS; TRANSMEMBRANE REGIONS;

ESCHERICHIA COLI;

AMINO ACIDS;

2 OXOGLUTARIC ACID; AMINO ACID; DEAMINASE; GLUTAMIC ACID; MAGNESIUM CHLORIDE;

ARTICLE; CELL INCLUSION; CODON; ESCHERICHIA COLI; MEMBRANE BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLLUTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTEUS MIRABILIS; PURIFICATION; TEMPERATURE;

AMIDOHYDROLASES; BACTERIAL PROTEINS; ENZYME STABILITY; ESCHERICHIA COLI; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KETOGLUTARIC ACIDS; METALS, HEAVY; PROTEIN ENGINEERING; PROTEIN REFOLDING; PROTEUS MIRABILIS; RECOMBINANT PROTEINS; SOLUBILITY; TEMPERATURE;

ESCHERICHIA COLI; PROTEUS MIRABILIS;

EID: 84875108160     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.01.005     Document Type: Article

References (40)

1. Ahn M.Y., Ryu K.S., Lee Y.W., Kim Y.S. Cytotoxicity and l-amino acid oxidase activity of crude insect drugs. Archives of Pharmacal Research 2000, 23:477-481.
2. Baek J.O., Seo J.W., Kwon O., Seong S.L., Kim I.H., Kim C.H. Expression and characterization of a second l-amino acid deaminase isolated from Proteus mirabilis in Escherichia coli. Journal of Basic Microbiology 2011, 51:129-135.
3. Baek J.O., Seo J.W., Kwon O., Seong S.I., Kim I.H., Kim C.H. Heterologous expression and characterization of l-amino acid deaminase from Proteus mirabilis in Escherichia coli. Chinese Journal of Biotechnology 2008, 24:21-29.
4. Barrett D.G., Yousaf M.N. Poly (triol a-ketoglutarate) as biodegradable, chemoselective, and mechanically tunable elastomers. Macromolecule 2008, 41:6347-6352.
5. Cooper A.J.L., Ginos J.Z., Meister A. Synthesis and properties of the α-keto acids. Chemical Reviews 1983, 83:321-358.
6. Chernyavskaya O.G., Shishkanova N.V., Il'chenko A.P., Finogenova T.V. Synthesis of alpha-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol. Applied Microbiology and Biotechnology 2000, 53:152-158.
7. Dodevski I., Pliüickthun A. Engineering integral membrane proteins for expression and stability. Production of membrane proteins: strategies for expression and isolation 2011, Wiley-VCH Verlag GmbH & KGaA, pp. 277-296.
8. Drechsel H.A., Thieken R., Jung G., Winkelmann G. α-Keto acids are novel siderophores in the genera Proteus, Providencia, and Morganella and are produced by amino acid deaminase. Journal of Bacteriology 1993, 175:2727-2733.
9. Finogenova T.V., Morgunov I.G., Chernyavskaya O.G. Organic acid production by the yeast Yarrowia lipolytica: a review of prospects. Applied Biochemistry & Microbiology 2005, 41:418-425.
10. Geueke B., Hummel W. A new bacterial l-amino acid oxidase with a broad substrate specificity: purification and characterization. Enzyme and Microbial Technology 2002, 31:77-87.
11. Hirotsu K., Goto M., Okamoto A., Miyahara I. Dual substrate recognition of aminotransferases. The Chemical Record 2005, 5:160-172.
12. Holz M., Otto C., Kretzschmar A., Yovkova V., Aurich A., Pötter M., Marx A., Barth G. Overexpression of alpha-ketoglutarate dehydrogenase in Yarrowia lipolytica and its effect on production of organic acids. Applied Microbiology and Biotechnology 2011, 89:1519-1526.
13. Hummel W., Schütte H., Schmidt E., Wandrey C., Kula M.R. Isolation of l-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of l-phenylalanine. Applied Microbiology and Biotechnology 1987, 26:409-416.
14. Il'chenko A.P., Lysyanskaya V.Y., Finogenova T.V., Morgunov I.G. Characteristic properties of metabolism of the yeast Yarrowia lipolytica during the synthesis of alpha-ketoglutaric acid from ethanol. Microbiologiia 2010, 79:450-455.
15. Il'chenko A.P., Chernyavskaya O.G., Shishkanova N.V., Finogenova T.V. Biochemical characterization of the yeast Yarrowia lipolytica overproducing carboxylic acids from ethanol: nitrogen metabolism enzymes. Microbiologiia 2003, 72:470-475.
16. Il'chenko A.P., Chernyavskaya O.G., Shishkanova N.V., Finogenova T.V. Metabolic characteristics of the mutant Yarrowia lipolytica strain N1 producing alpha-ketoglutaric and citric acids from ethanol and the effect of NH4+ and O-2 on yeast respiration and acidogenesis. Microbiologiia 2001, 70:151-157.
17. Il'chenko A.P., Chernyavskaya O.G., Shishkanova N.V., Finogenova T.V. Metabolism of Yarrowia lipolytica grown on ethanol under conditions promoting the production of alpha-ketoglutaric and citric acids: a comparative study of the central metabolism enzymes. Microbiologiia 2002, 71:269-274.
18. Kim H., Na S.H., Lee S.Y., Jeong Y.M., Hwang H.J., Hur J.Y., Park S.H., Woo J.C., Kim S.G. Structure-function studies of a plant tyrosyl-DNA phosphodiesterase provide novel insights into DNA repair mechanisms of Arabidopsis thaliana. Biochemical Journal 2012, 443:49-56.
19. Kwon K.S., Lee S., Yu M.H. Refolding of alpha 1-antitrypsin expressed as inclusion bodies in Escherichia coli: characterization of aggregation. Biochimica Biophysica Acta 1995, 1247:179-184.
20. Koepsell H.J., Stodola F.H., Sharpe E.S. Production of α-ketoglutarate in glucose oxidation by Pseudomonas fluorescens. Journal of the American Chemical Society 1952, 74:5142-5144.
21. Lin L.L., Chien H.R., Wang W.C., Hwang T.S., Fu H.M., Hsu W.H. Expression of Trigonopsis variabilis d-amino acid oxidase gene in Escherichia coli and characterization of its inactive mutants. Enzyme and Microbial Technology 2000, 27:482-491.
22. Liu L.M., Li Y., Zhu Y., Du G.C., Chen J. Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level. Metabolic Engineering 2007, 9:21-29.
23. Massad G., Zhao H., Mobley H.T. Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad. Journal of Bacteriology 1995, 177:5878-5883.
24. P. Maldonado, J.P. Desmarquest, C. Gaillardin, D. Binet, Yeasts, Institut Français du Petrole, US Patent 3,930,946 (1976).
25. Nannenga B.L., Baneyx F. Enhanced expression of membrane proteins in E. coli with a P(BAD) promoter mutant: synergies with chaperone pathway engineering strategies. Microbial Cell Factories 2011, 10:105.
26. Ödman P., Wellborn W.B., Bommarius A.S. An enzymatic process to α-ketoglutarate from l-glutamate: the coupled system l-glutamate dehydrogenase/NADH oxidase. Tetrahedron Asymmetry 2004, 15:2933-2937.
27. Otto C., Yovkova V., Barth G. Overproduction and secretion of α-ketoglutaric acid by microorganisms. Applied Microbiology and Biotechnology 2011, 92:689-695.
28. Patra A.K., Gahlay G.K., Reddy B.V. Refolding, structural transition and spermatozoa-binding of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli. European Journal of Biochemistry 2000, 267:7075-7081.
29. Patra A.K., Mukhopadhyay R., Mukhija R. Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli. Protein Expression and Purification 2000, 18:182-192.
30. Pantaleone D.P., Geller A.M., Taylor P.P. Purification and characterization of an l-amino acid deaminase used to prepare unnatural amino acids. Journal of Molecular Catalysis B: Enzymatic 2001, 11:795-803.
31. Sahdev S., Khattar S.K., Saini K.S. Production of active eukaryotic proteins through bacterial expression systems: a review of existing biotechnology strategies. Molecular and Cellular Biochemistry 2008, 307:249-264.
32. Schadewaldt P., Adelmeyer F. Coupled enzymatic assay for estimation of branched-chain l-amino acid aminotransferase activity with 2-oxo acid substrates. Analytical Biochemistry 1996, 238:65-71.
33. Stottmeister U., Aurich A., Wilde H., Andersch J., Schmidt S., Sicker D. White biotechnology for green chemistry: fermentative 2-oxocarboxylic acids as novel building blocks for subsequent chemical syntheses. Journal of Industrial Microbiology and Biotechnology 2005, 32:651-664.
34. Sun M.Z., Guo C., Tian Y., Chen D., Greenaway F.T., Liu S. Biochemical, functional and structural characterization of Akbu-LAAO: a novel snake venom l-amino acid oxidase from Agkistrodon blomhoffii ussurensis. Biochimie 2010, 92:343-349.
35. Takahashi E., Ito K., Yochimoto T. Cloning of L-amino acid deaminase gene from Proteus vulgaris. Bioscience, Biotechnology, and Biochemistry 1999, 63:2244-2247.
36. Verseck S, Karau A, Weber M, Fermentative production of alpha-ketoglutaric acid, Evonik Degussa GmbH, Patent WO200905348 (2009).
37. Wagner S., Klepsch M.M., Schlege, l S., Appel A., Draheim R., Tarry M., Hogbom M., van Wijk K.J., Slotboom D.J., Persson J.O., de Gier J.W. Tuning Escherichia coli for membrane protein overexpression. Proceedings of the National Academy of Sciences of the United States of America 2008, 105:14371-14376.
38. E. Weissbrodt, M. Gey, G. Barth, H. Weber, U. Stottmeister, R. Düresch, H.P. Richter, Verfahren zur Herstellung von 2-Oxoglutarsäure durch Hefen, DD Patent 267,999 (1989).
39. Zhou J., Zhou H., Du G.C., Liu L.M., Chen J. Screening of a thiamine auxotrophic yeast for alpha-ketoglutaric acid overproduction. Letters in Applied Microbiology 2010, 51:264-271.
40. Zhang D.D., Liang N., Shi Z.P., Liu L.M., Chen J., Du G.C. Enhancement of alpha-ketoglutarate production in Torulopsis glabrata: Redistribution of carbon flux from pyruvate to alpha-ketoglutarate. Biotechnology and Bioprocess Engineering 2009, 14:134-139.