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Volumn , Issue , 2011, Pages 275-296

Engineering Integral Membrane Proteins for Expression and Stability

Author keywords

Expression; G protein coupled receptor; Integral membrane protein; Protein engineering; Stability

Indexed keywords


EID: 84886125373     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9783527634521.ch10     Document Type: Chapter
Times cited : (3)

References (19)
  • 1
    • 0028050350 scopus 로고
    • Rapid evolution of a protein in vitro by DNA shuffling
    • Stemmer, W.P. (1994) Rapid evolution of a protein in vitro by DNA shuffling . Nature , 370 , 389-391 .
    • (1994) Nature , vol.370 , pp. 389-391
    • Stemmer, W.P.1
  • 2
    • 0029869449 scopus 로고    scopus 로고
    • An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues
    • Zaccolo, M., Williams, D.M., Brown, D.M., and Gherardi, E. (1996) An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues . J. Mol. Biol. , 255 , 589-603 .
    • (1996) J. Mol. Biol. , vol.255 , pp. 589-603
    • Zaccolo, M.1    Williams, D.M.2    Brown, D.M.3    Gherardi, E.4
  • 3
    • 0028559783 scopus 로고
    • Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
    • Virnekäs, B., Ge, L., Plückthun, A., Schneider, K.C., Wellnhofer, G., and Moroney, S.E. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis . Nucleic Acids Res. , 22 , 5600-5607 .
    • (1994) Nucleic Acids Res. , vol.22 , pp. 5600-5607
    • Virnekäs, B.1    Ge, L.2    Plückthun, A.3    Schneider, K.C.4    Wellnhofer, G.5    Moroney, S.E.6
  • 5
    • 54449087770 scopus 로고    scopus 로고
    • Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
    • USA
    • Sarkar, C.A., Dodevski, I., Kenig, M., Dudli, S., Mohr, A., Hermans, E., and Plückthun, A. (2008) Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity . Proc. Natl. Acad. Sci. USA , 105 , 14808-14813 .
    • (2008) Proc. Natl. Acad. Sci. , vol.105 , pp. 14808-14813
    • Sarkar, C.A.1    Dodevski, I.2    Kenig, M.3    Dudli, S.4    Mohr, A.5    Hermans, E.6    Plückthun, A.7
  • 6
    • 0029838532 scopus 로고    scopus 로고
    • Purification of a rat neurotensin receptor expressed in Escherichia coli
    • Tucker, J. and Grisshammer, R. (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli . Biochem. J. , 317 , 891-899 .
    • (1996) Biochem. J. , vol.317 , pp. 891-899
    • Tucker, J.1    Grisshammer, R.2
  • 7
    • 0031909113 scopus 로고    scopus 로고
    • Molecular evolution by staggered extension process (StEP) in vitro recombination
    • Zhao, H., Giver, L., Shao, Z., Affholter, J.A., and Arnold, F.H. (1998) Molecular evolution by staggered extension process (StEP) in vitro recombination . Nat. Biotechnol. , 16 , 258-261 .
    • (1998) Nat. Biotechnol. , vol.16 , pp. 258-261
    • Zhao, H.1    Giver, L.2    Shao, Z.3    Affholter, J.A.4    Arnold, F.H.5
  • 8
    • 0036185646 scopus 로고    scopus 로고
    • Constitutive activation of the neurotensin receptor 1 by mutation of Phe 358 in helix seven
    • Barroso, S., Richard, F., Nicolas-Etheve, D., Kitabgi, P., and Labbe-Jullie, C. (2002) Constitutive activation of the neurotensin receptor 1 by mutation of Phe 358 in helix seven . Br. J. Pharmacol. , 135 , 997-1002 .
    • (2002) Br. J. Pharmacol. , vol.135 , pp. 997-1002
    • Barroso, S.1    Richard, F.2    Nicolas-etheve, D.3    Kitabgi, P.4    Labbe-jullie, C.5
  • 9
    • 0032568928 scopus 로고    scopus 로고
    • Mutagenesis and modeling of the neurotensin receptor NTR1. Identification of residues that are critical for binding SR 48692, a nonpeptide neurotensin antagonist
    • Labbe-Jullie, C., Barroso, S., Nicolas-Eteve, D., Reversat, J.L., Botto, J.M., Mazella, J., Bernassau, J.M., and Kitabgi, P. (1998) Mutagenesis and modeling of the neurotensin receptor NTR1. Identification of residues that are critical for binding SR 48692, a nonpeptide neurotensin antagonist . J. Biol. Chem. , 273 , 16351-16357 .
    • (1998) J. Biol. Chem. , vol.273 , pp. 16351-16357
    • Labbe-jullie, C.1    Barroso, S.2    Nicolas-eteve, D.3    Reversat, J.L.4    Botto, J.M.5    Mazella, J.6    Bernassau, J.M.7    Kitabgi, P.8
  • 11
    • 0242637558 scopus 로고    scopus 로고
    • Fluorescent ligands, antibodies, and proteins for the study of receptors
    • Daly, C.J. and McGrath, J.C. (2003) Fluorescent ligands, antibodies, and proteins for the study of receptors . Pharmacol. Ther. , 100 , 101-118 .
    • (2003) Pharmacol. Ther. , vol.100 , pp. 101-118
    • Daly, C.J.1    McGrath, J.C.2
  • 13
    • 0033538418 scopus 로고    scopus 로고
    • Changing single side-chains can greatly enhance the resistance of a membrane protein to irreversible inactivation
    • Lau, F.W., Nauli, S., Zhou, Y., and Bowie, J.U. (1999) Changing single side-chains can greatly enhance the resistance of a membrane protein to irreversible inactivation . J. Mol. Biol. , 290 , 559-564 .
    • (1999) J. Mol. Biol. , vol.290 , pp. 559-564
    • Lau, F.W.1    Nauli, S.2    Zhou, Y.3    Bowie, J.U.4
  • 14
    • 0034629489 scopus 로고    scopus 로고
    • Building a thermostable membrane protein
    • Zhou, Y. and Bowie, J.U. (2000) Building a thermostable membrane protein . J. Biol. Chem. , 275 , 6975-6979 .
    • (2000) J. Biol. Chem. , vol.275 , pp. 6975-6979
    • Zhou, Y.1    Bowie, J.U.2
  • 16
    • 38949158505 scopus 로고    scopus 로고
    • Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form
    • USA
    • SerraNo-Vega, M.J., Magnani, F., Shibata, Y., and Tate, C.G. (2008) Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form . . Proc. Natl. Acad. Sci. USA , 105 , 877-882 .
    • (2008) Proc. Natl. Acad. Sci. , vol.105 , pp. 877-882
    • Serrano-vega, M.J.1    Magnani, F.2    Shibata, Y.3    Tate, C.G.4
  • 17
    • 49449114407 scopus 로고    scopus 로고
    • Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor
    • USA
    • Magnani, F., Shibata, Y., Serrano-Vega, M.J., and Tate, C.G. (2008) Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor . . Proc. Natl. Acad. Sci. USA , 105 , 10744-10749 .
    • (2008) Proc. Natl. Acad. Sci. , vol.105 , pp. 10744-10749
    • Magnani, F.1    Shibata, Y.2    Serrano-vega, M.J.3    Tate, C.G.4
  • 19
    • 79955021788 scopus 로고    scopus 로고
    • Evolution of three human GPCRs for higher expression and stability
    • in press.
    • Dodevski, I. and Plückthun, A. (2011) Evolution of three human GPCRs for higher expression and stability . J. Mol. Biol. , in press.
    • (2011) J. Mol. Biol.
    • Dodevski, I.1    Plückthun, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.