Crude saponins from Platycodon grandiflorum induce apoptotic cell death in RC-58T/h/SA#4 prostate cancer cells through the activation of caspase cascades and apoptosis-inducing factor

Oncology Reports

Volumn 29, Issue 4, 2013, Pages 1421-1428

  Lee, Ju Hye ^a   Oh, Eun Kyoung ^a   Cho, Hyun Dong ^a   Kim, Jae Yong ^b   Lee, Mi Kyung ^a   Seo, Kwon Il ^a  

^a National University   (South Korea)

^b Sunchon National University   (South Korea)

Author keywords

Apoptosis; Apoptosis inducing factor; Caspase; Platycodon grandiflorum; Primary prostate cancer cells

Indexed keywords

APOPTOSIS INDUCING FACTOR; BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; BUTANOL; CASPASE; CASPASE INHIBITOR; DIAION HP 20 RESIN; PROTEIN BAX; RESIN; SAPONIN; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL; CELL CYCLE G1 PHASE; CELL LINE; CELL PROLIFERATION; CELL STRAIN RC 58T H SA 4; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA FRAGMENTATION; DOWN REGULATION; DRUG CYTOTOXICITY; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; MITOCHONDRION; PLATYCODON; PRIORITY JOURNAL; PROSTATE CANCER; UPREGULATION;

ANTINEOPLASTIC AGENTS; APOPTOSIS; APOPTOSIS INDUCING FACTOR; BCL-2-ASSOCIATED X PROTEIN; CASPASES; CELL LINE, TUMOR; DNA FRAGMENTATION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, BCL-2; HUMANS; MALE; MITOCHONDRIA; PLANT EXTRACTS; PLATYCODON; PROSTATIC NEOPLASMS; SAPONINS; UP-REGULATION;

EID: 84874753969     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2013.2256     Document Type: Article

References (50)

1. Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statis¬tics. CA Cancer J Clin 55: 74-108, 2002.
2. Pavone-Macaluso M, Carruba G and Castagnetta L: Steroid receptors in prostate cancer tissues and cells: pathophysiology, problems in methodology, clinical value and controversial questions. Arch Esp Urol 47: 189-201, 1994.
3. Lee DH, Szczepanski M and Lee YJ: Role of Bax in quercetin-induced apoptosis in human prostate cancer cells. Biochem Pharmacol 75: 2345-2355, 2008.
4. Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Chu TM, Mirand EA and Murphy GP: LNCaP model of human prostatic carcinoma. Cancer Res 43: 1809-1818, 1983.
5. Nagle RB, Ahmann FR, McDaniel KM, Paquin ML, Clark VA and Celniker A: Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Res 47: 281-286, 1987.
6. Mickey DD, Stone KR, Wunderli H, Mickey GH, Vollmer RT and Paulson DF: Heterotransplantation of a human prostatic adeno¬carcinoma cell line in nude mice. Cancer Res 37: 4049 4058, 1977.
7. Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF and Jones LW: Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol 17: 16-23, 1979.
8. Choi SR, Lee JH, Kim JY, Park KW, Jeong IY, Shim KH, Lee MK and Seo KI: Decursin from Angelicagigas Nakai induces apoptosis in RC-58T/h/SA#4 primary human prostate cancer cells via a mitochondria-related caspase pathway. Food Chem Toxicol 49: 2517-2523, 2011.
9. Yasunaga Y, Nakamura K, Ko D, Srivastava S, Moul JW, Sesterhenn IA, McLeod DG and Rhim JS: A novel human cancer culture model for the study of prostate cancer. Oncogene 20: 8036-8041, 2001.
10. Gu Y, Li H, Miki J, Kim KH, Furusato B, Sesterhenn IA, Chu WS, McLeod DG, Srivastava S, Ewing CM, Isaacs WB and Rhim JS: Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines. Exp Cell Res 312: 831-843, 2005.
11. Gu Y, Kim KH, KO D, Nakamura K, Yasunaga Y, Moul JW, Srivastava S, Arnstein P and Rhim JS: A telomerase-immor¬talized primary human prostate cancer clonal cell line with neoplastic phenotypes. Int J Oncol 25: 1057-1064, 2004.
12. Sung NJ, Lee SJ, Shin JH, Lee IS and Chung YC: Effects of Platycodon grandiflorum extract on blood glucose and lipid composition in alloxan induced hyperglycemic rats. J Korean Soc Food Sci Nutr 25: 986-992, 1996.
13. Fu W, Dou DQ, Shimizu N, Takeda T, Pei YH and Chen YJ: Studies on the chemical constituents from the roots of Platycodon grandiflorum. J Nat Med 60: 68-72, 2005.
14. Ishii H, Tori K, Tozyo T and Yoshimura Y: Saponin from roots of Platycodon grandiflorum. Part 2. Isolation and structure of new triterpene glycosides. J Chem Soc Perkin Trans 1: 662-668, 1984.
15. Nikaido T, Koike K, Mitsunaga K and Saeki T: Two new trit¬erpenoid saponin from Platycodon grandiflorum. Chem Pharm Bull 47: 903-904, 1999.
16. Saeki T, Koike K and Nikaido T: A Comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum. Planta Med 65: 428-431, 1999.
17. Ozaki Y: Studies on antiinflammatory effect of Japanese Oriental medicines (kampo medicines) used to treat inflamma¬tory diseases. Biol Pharm Bull 18: 559-562, 1995.
18. Lee JY and Lee SJ: Surface activities of ginseng saponins and their interactions with biomolecules. Korean J Ginseng Sci 19: 122-126, 1995.
19. Park KU, Wee JJ, Kim JY, Jeong CH, Kang KS, Cho YS and Seo KI: Anticancer and immuno-activities of edible crude saponin from soybean cake. J Korean Soc Food Sci Nutr 34: 1509-1513, 2005.
20. Kwak YS, Kyung JS, Kim SK and Wee JJ: An isolation of crude saponin from red-ginseng efflux by Diaion HP-20 resin adsorp-tion method. J Korean Soc Food Sci Nutr 30: 1-5, 2001.
21. Kim CH, Jung BY, Jung SK, Lee CH, Lee HS, Kim BH and Kim SK: Evaluation of antioxidant activity of Platycodon gran-diflorum. J Environ Toxicol 25: 85-94, 2010.
22. Han SB, Park SH, Lee KH, Lee CW, Lee SH, Kim HC, Kim YS, Lee HS and Kim HM: Polysaccharide isolated from the radix of Platycodon grandiflorum selectively activates B cells and macrophages but not T cells. Int Immunophamacol 1: 1969-1978, 2001.
23. Kim HS, Kim GJ and Kim HS: Effect of the feeding Platycodon grandiflorum on lipid components of liver and liver function in hypercholesterolemia rats. Korean J Food Nutr 11: 312-318, 1998.
24. Xie Y, Deng W, Sun H and Li D: Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses. Int Immunopharmacol 8: 1143-1150, 2008.
25. Lee KJ, Hwang SJ, Choi JH and Jeong HG: Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF-?B activation via ROS signal pathway. Cancer Lett 268: 233-243, 2008.
26. Yu JS and Kim AK: Platycodin D induces apoptosis in MCF-7 human breast cancer cells. J Med Food 13: 298-305, 2010.
27. Choi JH, Hwang YP, Han ES, Kim HG, Park BH, Lee HS, Park BK, Lee YC, Chung YC and Jeong HG: Inhibition of acrolein-stimulated MUC5AC expression by Platycodon grandiflorum root-derived saponin in A549 cells. Food Chem Toxicol 49: 2157-2166, 2011.
28. Shin DY, Kim GY, Li W, Choi BT, Kim ND, Kang HS and Choi YH: Implication of intracellular ROS formation, caspase-3 activation and Egr-1 induction in platycodon D-induced apoptosis of U937 human leukemia cells. Biomed Phamacother 63: 86-94, 2008.
29. Ye R, Zhang X, Kong X, Han J, Yang Q, Zhang Y, Chen Y, Li P, Liu J, Shi M, Xiong L and Zhao G: Ginsenoside Rd attenu¬ates mitochondrial dysfunction and sequential apoptosis after transient focal ischemia. Neuroscience 178: 169-180, 2011.
30. Kim JY, Park KW, Moon KD, Lee MK, Choi JA, Yee ST, Shim KH and Seo KI: Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi radix. Food Chem Toxicol 46: 3753-3758, 2008.
31. Sun H, Chen L, Wang J, Wang K and Zhou J: Structure-function relationship of the saponins from the roots of Platycodon grandiflorum for hemolytic and adjuvant activity. Int Immunopharmacol 11: 2047-2056, 2011.
32. Chan PK: Acylation with diangeloyl groups at C21-22 positions in triterpenoid saponins is essential for cytotoxcity towards tumor cells. Biochem Pharmacol 73: 341-350, 2007.
33. Lee YH, Kim YJ, Kim HI, Cho HY and Yoon HG: Potent HAT inhibitory effect of aqueous extract from Bellflower (Platycodon grandiflorum) roots on androgen receptor-mediated transcrip¬tional regulation. Food Sci Biotechnol 16: 457-462, 2007.
34. Xie Z, Huang H, Zhao Y, Shi H, Wangc S, Wang TTY, Chen P and Yu L: Chemical composition and anti-proliferative and anti-inflammatory effects of the leaf and whole-plant samples of diploid and tetraploid Gynostemma pentaphyllum (Thunb.) Makino. Food Chem 132: 125-133, 2012.
35. Kim YS, Kim JS, Choi SU, Kim JS, Lee HS, Roh SH, Jeong YC, Kim YK and Ryu SY: Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines. Planta Med 71: 566-568, 2005.
36. Duperez L, Wirawan E, Vanden Berqhe T and Vandenabeele P: Major cell death pathways at a glance. Microbes Infect 11: 1050 1062, 2009.
37. Park DS, Stefanis L and Greene LA: Ordering the multiple pathways of apoptosis. Trends Cardiovasc Med 7: 294-301, 1997.
38. Harada H and Grant S: Apoptosis regulators. Rev Clin Exp Hematol 7: 117-138, 2003.
39. Kischkel FC, Hellbardt S, Behrmann I, Germer M, Pawlita M, Krammer PH and Peter ME: Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J 14: 5579-5588, 1995.
40. Medema JP, Scaffidi C, Kischkel FC, Shevchenko A, Mann M, Krammer PH and Peter ME: FLICE is activated by associa¬tion with the CD95 death-inducing signaling complex (DISC). EMBO J 16: 2794-2804, 1997.
41. Kroemer G, Zamzami N and Susin SA: Mitochondrial control of apoptosis. Immunol Today 18: 44-51, 1997.
42. Zou H, Henzel WJ, Liu X, Lutschg A and Wang X: Apaf-1, a human protein homologous to C. elegans CED-4 participates in cytochrome c-dependent activation of caspase-3. Cell 90: 405-413, 1997.
43. Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD: The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 275: 1132-1136, 1997.
44. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X: Cytochrome c and dATP-dependent formation of Apaf-1/caspase9 complex initiates an apoptotic protease cascade. Cell 91: 479-489, 1997.
45. Kantari C and Walczak H: Caspase-8 and bid: caught in the act between death receptors and mitochondria. Biochim Biophys Acta 1813: 558-563, 2011.
46. Gross A, Yin XM, Wang K, Wei MC, Jockel J, Milliman C, Erdjument-Bromage H, Tempst P and Korsmeyer SJ: Caspase cleaved BID targets mitochondria and is required for cyto¬chrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death. J Biol Chem 274: 1156-1163, 1999.
47. Jin S and Dai CL: Attenuation of reperfusion-induced hepato¬cyte apoptosis is associated with reversed bcl-2/bax ratio in hemi-hepatic artery-preserved portal occlusion. J Surg Res 174: 298-304, 2011.
48. Zhang M, Liu H, Tian Z, Griffith BN, Ji M and Li QQ: Gossypol induces apoptosis in human PC-3 prostate cancer cells by modu¬lating caspase-dependent and caspase-independent cell death pathways. Life Sci 80: 767-774, 2007.
49. Kim SY, Park KW, Kim JY, Jeong IY, Byun MW, Park JE, Yee ST, Kim KH, Rhim JS, Yamada K and Seo KI: Thiosulfinates from Allium tuberosum L. induce apoptosis via caspase-dependent and-independent pathways in PC-3 human prostate cancer cells. Bioorg Med Chem Lett 18: 199-204, 2008.
50. Cande C, Cohen I, Daugas E, Ravaqnan L, Larochette N, Zamzami N and Kroemer G: Apoptosis-inducing factor (AIF): a novel caspase-independent death effector released from mito¬chondria. Biochimie 84: 215-222, 2002.