Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing

Methods in Enzymology

Volumn 523, Issue , 2013, Pages 327-349

  McLaughlin, Megan E ^a   Sidhu, Sachdev S ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Deep sequencing; Directed evolution; Peptide recognition domain; Phage display; Protein engineering; Specificity

Indexed keywords

ANTIBODY CONJUGATE; BETA LACTAMASE; COAT PROTEIN; DNA DIRECTED DNA POLYMERASE BETA; DNA FRAGMENT; HETERODUPLEX; OLIGONUCLEOTIDE; PEPTIDES AND PROTEINS; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; POLYNUCLEOTIDE 5' HYDROXYL KINASE; RECOMBINANT PROTEIN; SINGLE STRANDED DNA; TAQ POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CENTRIFUGE; COMBINATORIAL LIBRARY; ELECTROPORATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HIGH THROUGHPUT SEQUENCING; MOLECULAR WEIGHT; NONHUMAN; NUCLEIC ACID SYNTHESIS; PDZ DOMAIN; PEPTIDE LIBRARY; PHAGE DISPLAY; PHAGEMID; POSITION WEIGHT MATRIX; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; STOP CODON;

EID: 84874087755     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-394292-0.00015-1     Document Type: Chapter

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