A method for generating sticky-end PCR products which facilitates unidirectional cloning and the one-step assembly of complex DNA constructs

Plasmid

Volumn 59, Issue 3, 2008, Pages 155-162

  Walker, Andrew ^a   Taylor, James ^b   Rowe, Duncan ^c   Summers, David ^c  

^a Geneservice Ltd   (United Kingdom)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Department of Genetics ^*  (United Kingdom)

Author keywords

Biobrick; PCR; Plasmid; Unidirectional cloning; Vector

Indexed keywords

PHENFORMIN; RESTRICTION ENDONUCLEASE;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GENE CASSETTE; HETERODUPLEX ANALYSIS; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID;

CLONING, MOLECULAR; DNA; DNA PRIMERS; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; MODELS, GENETIC; MOLECULAR BIOLOGY; PLASMIDS; POLYMERASE CHAIN REACTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 41549107019     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2008.02.002     Document Type: Article

References (13)

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