Selection of Appropriate Reference Genes for RT-qPCR Analysis in a Streptozotocin-Induced Alzheimer's Disease Model of Cynomolgus Monkeys (Macaca fascicularis)

PLoS ONE

Volumn 8, Issue 2, 2013, Pages

  Park, Sang Je ^a,b   Kim, Young Hyun ^a   Lee, Youngjeon ^a,c   Kim, Kyoung Min ^a   Kim, Heui Soo ^b   Lee, Sang Rae ^a   Kim, Sun Uk ^a   Kim, Sang Hyun ^a   Kim, Ji Su ^a   Jeong, Kang Jin ^a   Lee, Kyoung Min ^d   Huh, Jae Won ^a   Chang, Kyu Tae ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Pusan National University   (South Korea)

^c Inje University   (South Korea)

^d Seoul National University Hospital   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; RNA; STREPTOZOCIN;

ACTB GENE; ALZHEIMER DISEASE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APP GENE; ARTICLE; BRAIN TISSUE; COMPUTER PROGRAM; CONTROLLED STUDY; FEMALE; GADPH GENE; GAPDH GENE; GENE; GENE EXPRESSION; GENETIC STABILITY; MACACA FASCICULARIS; MAPT GENE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; RPS19 GENE; RSP19 GENE; YWHAZ GENE;

ALZHEIMER DISEASE; ANIMALS; BRAIN; GENE EXPRESSION PROFILING; GENES; MACACA FASCICULARIS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; STREPTOZOCIN;

EID: 84874009086     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0056034     Document Type: Article

References (32)

1. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.
2. Mafra V, Kubo KS, Alves-Ferreira M, Ribeiro-Alves M, Stuart RM, et al. (2012) Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions. PLoS One 7: e31263.
3. Yperman J, De Visscher G, Holvoet P, Flameng W, (2004) Beta-actin cannot be used as a control for gene expression in ovine interstitial cells derived from heart valves. J Heart Valve Dis 13: 848-853.
4. Park SJ, Huh JW, Kim YH, Lee SR, Kim SH, et al. (2012) Selection of Internal Reference Genes for Normalization of Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Analysis in the Canine Brain and Other Organs. Mol Biotechnol. In press.
5. Lester-Coll N, Rivera EJ, Soscia SJ, Doiron K, Wands JR, et al. (2006) Intracerebral streptozotocin model of type 3 diabetes: relevance to sporadic Alzheimer's disease. J Alzheimers Dis 9: 13-33.
6. Grunblatt E, Salkovic-Petrisic M, Osmanovic J, Riederer P, Hoyer S, (2007) Brain insulin system dysfunction in streptozotocin intracerebroventricularly treated rats generates hyperphosphorylated tau protein. J Neurochem 101: 757-770.
7. Mistur R, Mosconi L, Santi SD, Guzman M, Li Y, et al. (2009) Current Challenges for the Early Detection of Alzheimer's Disease: Brain Imaging and CSF Studies. J Clin Neurol 5: 153-166.
8. Heo JH, Lee SR, Lee ST, Lee KM, Oh JH, et al. (2011) Spatial distribution of glucose hypometabolism induced by intracerebroventricular streptozotocin in monkeys. J Alzheimers Dis 25: 517-523.
9. Andersen CL, Jensen JL, Orntoft TF, (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250.
10. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26: 509-515.
11. Li W, Wu Y, Min F, Li Z, Huang J, et al. (2010) A nonhuman primate model of Alzheimer's disease generated by intracranial injection of amyloid-beta42 and thiorphan. Metab Brain Dis 25: 277-284.
12. Kriks S, Shim JW, Piao J, Ganat YM, Wakeman DR, et al. (2011) Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease. Nature 480: 547-551.
13. Ma Y, Peng S, Spetsieris PG, Sossi V, Eidelberg D, et al. (2012) Abnormal metabolic brain networks in a nonhuman primate model of parkinsonism. J Cereb Blood Flow Metab 32: 633-642.
14. McBride JL, Pitzer MR, Boudreau RL, Dufour B, Hobbs T, et al. (2011) Preclinical safety of RNAi-mediated HTT suppression in the rhesus macaque as a potential therapy for Huntington's disease. Mol Ther 19: 2152-2162.
15. Uchida A, Sasaguri H, Kimura N, Tajiri M, Ohkubo T, et al. (2012) Non-human primate model of amyotrophic lateral sclerosis with cytoplasmic mislocalization of TDP-43. Brain 135: 833-846.
16. Ahn K, Huh JW, Park SJ, Kim DS, Ha HS, et al. (2008) Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues. BMC Mol Biol 9: 78.
17. Noriega NC, Kohama SG, Urbanski HF, (2009) Gene expression profiling in the rhesus macaque: methodology, annotation and data interpretation. Methods 49: 42-49.
18. Noriega NC, Kohama SG, Urbanski HF, (2010) Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain. BMC Mol Biol 11: 47.
19. Ferguson BS, Nam H, Hopkins RG, Morrison RF, (2010) Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes. PLoS One 5: e15208.
20. Everaert BR, Boulet GA, Timmermans JP, Vrints CJ, (2011) Importance of suitable reference gene selection for quantitative real-time PCR: special reference to mouse myocardial infarction studies. PLoS One 6: e23793.
21. Rivera EJ, Goldin A, Fulmer N, Tavares R, Wands JR, et al. (2005) Insulin and insulin-like growth factor expression and function deteriorate with progression of Alzheimer's disease: link to brain reductions in acetylcholine. J Alzheimers Dis 8: 247-268.
22. Steen E, Terry BM, Rivera EJ, Cannon JL, Neely TR, et al. (2005) Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer's disease-is this type 3 diabetes? J Alzheimers Dis 7: 63-80.
23. Chang Z, Ling C, Yamashita M, Welham NV, (2010) Microarray-driven validation of reference genes for quantitative real-time polymerase chain reaction in a rat vocal fold model of mucosal injury. Anal Biochem 406: 214-221.
24. Marum L, Miguel A, Ricardo CP, Miguel C, (2012) Reference gene selection for quantitative real-time PCR normalization in Quercus suber. PLoS One 7: e35113.
25. Tachaudomdach C, Kantachuvesiri S, Changsirikulchai S, Wimolluck S, Pinpradap K, et al. (2012) Connective tissue growth factor gene expression and decline in renal function in lupus nephritis. Exp Ther Med 3: 713-718.
26. Shen B, Dong P, Li D, Gao S, (2011) Expression and function of ABCG2 in head and neck squamous cell carcinoma and cell lines. Exp Ther Med 2: 1151-1157.
27. Saigusa S, Inoue Y, Tanaka K, Toiyama Y, Kawamura M, et al. (2012) Significant correlation between LKB1 and LGR5 gene expression and the association with poor recurrence-free survival in rectal cancer after preoperative chemoradiotherapy. J Cancer Res Clin Oncol. In press.
28. Yuasa K, Takeda S, Hijikata T, (2012) A conserved regulatory element located far downstream of the gls locus modulates gls expression through chromatin loop formation during myogenesis. FEBS Lett 586: 3464-3470.
29. Miura C, Sugawara K, Neriya Y, Minato N, Keima T, et al. (2012) Functional characterization and gene expression profiling of superoxide dismutase from plant pathogenic phytoplasma. Gene. In press.
30. Rozen S, Skaletsky H, (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365-386.
31. Huh JW, Kim YH, Park SJ, Kim DS, Lee SR, et al. (2012) Large-scale transcriptome sequencing and gene analyses in the crab-eating macaque (macaca fascicularis) for biomedical research. BMC Genomics 13: 163.
32. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55: 611-622.