Assessment of Long-Term Effects of Nanoparticles in a Microcarrier Cell Culture System

PLoS ONE

Volumn 8, Issue 2, 2013, Pages

  Mrakovcic, Maria ^a   Absenger, Markus ^a   Riedl, Regina ^a   Smole, Claudia ^a   Roblegg, Eva ^b   Fröhlich, Leopold F ^a   Fröhlich, Eleonore ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

MULTI WALLED NANOTUBE; NANOPARTICLE; POLYSTYRENE;

APOPTOSIS; ARTICLE; BASEMENT MEMBRANE; CELL COUNT; CELL CULTURE; CELL DEATH; CELL DENSITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOTOXICITY; ENDOTHELIUM CELL; LYSOSOME; MICROCARRIER CULTURE;

BIOLOGICAL TRANSPORT; CELL CULTURE TECHNIQUES; CELL DEATH; CELL LINE; HUMANS; INTRACELLULAR SPACE; MICROTECHNOLOGY; NANOPARTICLES; POLY(ADP-RIBOSE) POLYMERASES; POLYSTYRENES; TIME FACTORS; TOXICITY TESTS;

EID: 84874007002     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0056791     Document Type: Article

References (46)

1. The European Commission. Commission Recommendation of 18 October 2011 on the definition of nanomaterials. Official Journal of the European Union (2011/696/EU). http://eur-lex.europa.eu/LexUriServ/LexuriServ.do?uri=OJ:L:2011:275:0038:0040:EN:PDF.
2. Lewinski N, Colvin V, Drezek R, (2008) Cytotoxicity of Nanoparticles. Small 4(1): 26-49.
3. Whitesides GM, (2003) The 'right' size in nanobiotechnology. Nature Biotech. 21(10): 1161-1165.
4. Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, et al. (2011) Nanotoxicology and nanoparticle safety in biomedical designs. Int. J. Nanomedicine 6: 1117-1127.
5. Oberdörster G, Maynard A, Donaldson K, Castranova V, Fitzpatrick J, et al. (2005) Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Part. Fibre Toxicol 2doi: 10.1186/1743-8977-2-8.
6. Bihari P, Vippola M, Schultes S, Praetner M, Khandoga AG, et al. (2008) Optimized dispersion of nanoparticles for biological in vitro and in vivo studies. Part. Fibre Toxicol. 5: 14.
7. Dockery DW, Pope CA 3rd, Xu X, Spengler JD, Ware JH, et al (1993) An association between air pollution and mortality in six U.S. cities. N Engl J Med. 329: 1753-1759.
8. Oberdörster G, Ferin J, Finkelstein G, Wade P, Corson N, (1990) Increased pulmonary toxicity of ultrafine particles. 2. Lung lavage studies. J Aerosol Sci 21: 384-387.
9. Ferin J, Oberdörster G, Penney D, Sonderholm S, Gelein RM, et al. (1990) Increased pulmonary toxicity of ultrafine particles. 1. Particle clearance, translocation, morphology. J Aerosol Sci. 21: 381-384.
10. Oberdörster G, Gelein RM, Ferin J, Weiss B, (1995) Association of particulate air pollution and acute mortality: involvement of ultrafine particles? Inhal Toxicol. 7: 111-124.
11. Lison D, Lardot C, Huaux F, Zanetti G, Fubini B, (1997) Influence of particle surface area on the toxicity of insoluble manganese dioxide dusts. Arch Toxicol. 71: 725-729.
12. Donaldson K, Stone V, Gilmour PS, Brown DM, MacNee W, (2000) Ultrafine particles: mechanisms of lung injury. Phil Trans R Soc Lond A. 358: 2741-2749.
13. Nel A, Xia T, Madler L, Li N, (2006) Toxic potential of materials at the nanolevel. Science 311: 622-627.
14. Renwick LC, Donaldson K, Clouter A, (2001) Impairment of alveolar macrophage phagocytosis by ultrafine particles. Toxicol. Appl. Pharmacol. 172: 119-127.
15. Yang H, Liu C, Yang D, Zhang H, Xi Z, (2009) Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition. J. Appl. Toxicol. 29: 69-78.
16. Kishore AS, Surekha P, Murthy PB, (2009) Assessment of the dermal and ocular irritation potential of multi-walled carbon nanotubes by using in vitro and in vivo methods. Toxicol. Lett. 191: 268-274.
17. Seaton A, Tran L, Aitken R, Donaldson K, (2010) Nanoparticles, human health hazard and regulation. J. R. Soc. Interface 7: 119-129.
18. Rodriguez-Yañez Y, Muñoz B, Albores A (2012) Mechanisms of toxicity of carbon nanotubes. Toxicol mech Methods 29: doi:10.3109/15376516.2012.754534.
19. Seibert H, Balls M, Fentem JH, Bianchi V, Clothier RH, et al. (1996) Acute toxicity testing in vitro and the classification and labelling of chemicals. ATLA 24: 499-510.
20. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, et al. (2005) Quantum dots for live cells, in vivo imaging and diagnostics. Science 307: 538-544.
21. Turnherr T, Brandenberger C, Fischer K, Diener L, Manser P, et al. (2011) A comparison of acute and long-term effects of industrial multiwalled carbon nanotubes on human lung and immune cells in vitro. Toxicol. Lett. 200(3): 176-186.
22. Sadauskas E, Jacobsen NR, Danscher G, Stoltenberg M, Vogel U, et al. (2009) Biodistribution of gold nanoparticles in mouse lung following intratracheal instillation. Chem Cent J. 3: 16.
23. Kwon JT, Kim DS, Minai-Tehrani A, Hwang SK, Chang SH, et al. (2009) Inhaled fluorescent magnetic nanoparticles induced extramedullary hematopoiesis in the spleen of mice. J. Occup.Health 51: 423-431.
24. Lasagna-Reeves C, Gonzalez-Romero D, Barria MA, Olmedo I, Clos A, et al. (2010) Bioaccumulation and toxicity of gold nanoparticles after repeated administration in mice. Biochem Biophys Res Commun. 393: 649-655.
25. Pazos P, Fortaner S, Prieto P, (2002) Long-term in vitro toxicity models: comparisons between a flow-cell bioreactor, a static-cell bioreactor and static cell cultures. Altern Lab Anim. 30(5): 515-523.
26. Gebhardt R, Hengstler JG, Müller D, Glöckner R, Buenning P, et al. (2003) New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures. Drug Metab Rev. 35(2-3): 145-213.
27. Lock LT, Tzanakakis ES, (2009) Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture. Tissue eng Part A 15(8): 2051-2063.
28. Alves PM, Moreira JL, Rodrigues JM, Aunins JG, Carrondo JMT, (1996) Two-dimensional versus three-dimensional culture systems: Effects on growth and productivity of BHK cells. Biotechnol. Bioeng. 52: 429-432.
29. Rourou S, van der Ark A, van der Velden T, Kallel H, (2007) A microcarrier cell culture process for propagating rabies virus in Vero cells grown in a stirred bioreactor under fully animal component free conditions. Vaccine 25: 3879-3889.
30. Justice BA, Badr NA, Felder RA, (2009) 3D cell culture opens new dimensions in cell-based assays. Drug Discov. Today 14(1/2): 102-107.
31. Varani J, Piel F, Josephs S, Beals TF, Hillegas WJ, (1998) Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions. Cytotechnology 28: 101-109.
32. Butler M, Burgener A, Patrick M, Berry M, Moffatt D, et al. (2000) Application of a serum-free medium for the growth of Vero cells and the production of reovirus. Biotechnol. Prog. 16: 854-858.
33. Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, et al. (2009) Stirred bioreactors for the expansion of adult pancreatic stem cells. Ann. Anat. 191: 104-115.
34. Larson B, Berry D, Justice B, Gainer T, (2010) A novel 3D cell culture method for automated drug screening. Biotech international 22: 10-12.
35. Edgell CS, McDonald CC, Graham JB, (1983) Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. 80: 3734-3737.
36. Lindl T, Lewandowski B, Sheyrogg S, Staudte A, (2005) Evaluation of the in vitro cytotoxicities of 50 chemicals by using an electronic current exclusion method versus the neutral red uptake and MTT assays. Altern. Lab. Anim. 33: 591-601.
37. Swiech K, da Silva GMC, Zangirolami TC, Iemma MRC, Selistre-de-Araújo HS, et al. (2007) Evaluating kinetic and physiological features of rCHO-K1 cells cultured on microcarriers for production of a recombinant metalloprotease/disintegrin. Electronic Journal of Biotechnology 10(2): 201-210. Available: http://www.ejbiotechnology.info/content/vol10/issue2/full/15. Accessed 02 July 2012.
38. Xiao C, Huang Z, Li W, Hu X, Qu W, et al. (1999) High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore. Cytotechnology 30: 143-147.
39. Wu J, Liu W, Xue C, Zhou S, Lan F, et al. (2009) Toxicity and penetration of TiO2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposure. Toxicol. Lett. 191: 1-8.
40. Lee J, Lilly D, Doty C, Podsiadlo P, Kotov NA, (2009) In vitro toxicity testing of nanoparticles in 3D cell culture. Small 5(10): 1213-1221.
41. Fröhlich E, Samberger C, Kueznik T, Absenger M, Roblegg E, et al. (2009) Cytotoxicity of nanoparticles independent from oxidative stress. J. Toxicol. Sci. 34(4): 363-375.
42. van Nieuwenhuijze AEM, van Lopik T, Aarden LA, (2003) Time between onset of apoptosis and release of nucleosomes from apoptotic cells: putative implications for systemic lupus erythematosus. Ann. Rheum. Dis. 62: 10-14.
43. Guillouzo A, Corlu A, Aninat C, Glaise D, Morel F, et al. (2007) The human hepatoma HepaRG cells: A highly differentiated model for studies of liver metabolism and toxicity of xenobiotics. Chem. Biol. Interact. 168: 66-73.
44. Bokhari M, Carnachan RJ, Cameron NR, Przyborski SA, (2007) Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge. J. Anat. 211: 567-576.
45. Li C, Tian T, Nan K, Zhao N, Guo Y, et al. (2008) Survival advantages of multicellular spheroids vs. monolayers of HepG2 cells in vitro. Oncol. Rep. 20: 1465-1471.
46. Anderson N, Borlak J, (2006) Drug-induced phospholipidosis. FEBS Lett. 580(23): 5533-5540.