Stirred bioreactors for the expansion of adult pancreatic stem cells

Annals of Anatomy

Volumn 191, Issue 1, 2009, Pages 104-115

  Serra, Margarida ^a   Brito, Catarina ^a   Leite, Sofia B ^a   Gorjup, Erwin ^b   von Briesen, Hagen ^b   Carrondo, Manuel J T ^a,c   Alves, Paula M ^a  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^b FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^c UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

Adult stem cells; Expansion; Microcarriers; Pancreatic stem cells; Stirred bioreactors

Indexed keywords

CELL MARKER;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BIOPROCESS; CELL ADHESION; CELL AGGREGATION; CELL DIFFERENTIATION; CELL EXPANSION; CELL FUNCTION; CELL GROWTH; CELL POPULATION; CELL PROLIFERATION; CELL RENEWAL; CELL THERAPY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE TECHNIQUE; CYTOLOGY; FEASIBILITY STUDY; GROWTH RATE; INTERMETHOD COMPARISON; MEDICAL TECHNOLOGY; MICROCARRIER CULTURE; NONHUMAN; OXYGEN TENSION; PANCREAS CELL; PH; RAT; STEM CELL; STIRRED REACTOR; TEMPERATURE;

ADIPOCYTES; ADULT; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL DIVISION; EQUIPMENT DESIGN; HOMEOSTASIS; HUMANS; PANCREAS; REGENERATION; SKIN; STEM CELLS; TISSUE EXPANSION; TISSUE EXPANSION DEVICES;

EID: 58149314043     PISSN: 09409602     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aanat.2008.09.005     Document Type: Article

References (21)

1. Abranches E., Bekman E., Henrique D., and Cabral J.S. Expansion of mouse embryonic stem cells on microcarriers. Biotechnol. Bioeng. 96 (2007) 1211-1221
2. Alves P.M., Moreira J.L., Rodrigues J.M., Aunins J.G., and Carrondo M.J.T. Two-dimensional versus three-dimensional culture systems: Effects on growth and productivity of BHK cells. Biotechnol. Bioeng. 52 (1996) 429-432
3. Cameron C.M., Hu W.S., and Kaufman D.S. Improved development of human embryonic stem cell-derived embryoid bodies by stirred vessel cultivation. Biotechnol. Bioeng. 94 (2006) 938-948
4. Chawla M., Bodnar C.A., Sen A., Kallos M.S., and Behie L.A. Production of Islet-like structures from neonatal porcine pancreatic tissue in suspension bioreactors. Biotechnol. Prog. 22 2 (2006) 561-567
5. Cormier J.T., Nieden N.I.Z., Rancourt D.E., and Kallos M.S. Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors. Tissue Eng. 12 11 (2006) 3233-3245
6. Dang S.M., Kyba M., Perlingeiro R., Daley G.Q., and Zandstra P.W. Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems. Biotechnol. Bioeng. 78 4 (2002) 442-453
7. Fernandes M.A., Fernandes T.G., Diogo M.M., Lobato da Silva C., and Cabral J.S. Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system. J. Biotechnol. 132 2 (2007) 227-236 (31)
8. Fok E.Y.L., and Zandstra P.W. Shear-controlled single-step mouse embryonic stem cells and embryoid body-based differentiation. Stem Cells 23 (2005) 1333-1342
9. Gilbertson J.A., Sen A., Behie L.A., and Kallos M.S. Scaled-up production of mammalian neural precursor cell aggregates in computer-controlled suspension bioreactors. Biotechnol. Bioeng. 94 (2006) 783-792
10. Gokhale P.J., and Andrews P.W. A prospective on stem cell research. Semin. Reprod. Med. 24 5 (2006) 289-297
11. King J.A., and Miller W.M. Bioreactor development for stem cell expansion and controlled differentiation. Curr. Opin. Chem. Biol. 11 (2007) 394-398
12. Kruse C., Birth M., Rohwedel J., Goepel A., and Wedel T. Pluripotency of adult stem cells derived from human and rat pancreas. Appl. Phys. A 79 (2004) 1617-1624
13. Kruse C., Kajahn J., Petschnik A.E., MaaB A., Klink E., Rapoport D.H., and Wedel T. Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures. Ann Anat. 188 (2006) 503-517
14. Masters J.R.H. Anima Cell Culture: A Practical Approach. third ed. (2000), Oxford University Press, Oxford
15. Moreira J.L., Alves P.M., Aunins J.G., and Carrondo M.J.T. Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol. Bioeng. 46 (1995) 351-360
16. Santos S.S., Fonseca L.L., Monteiro M.A.R., Carrondo M.J.T., and Alves P.M. Culturing primary brain astrocytes under a fully controlled environment in a novel bioreactor. J. Neurosc. Res. 79 (2005) 26-32
17. Santos S.S., Leite S.B., Ursula Sonnewald U., Carrondo M.J.T., and Alves P.M. Stirred vessel cultures of rat brain cells aggregates: Characterization of major metabolic pathways and cell population dynamics. J. Neurosc. Res. 85 (2007) 3386-3397
18. Serra M., Leite S.B., Brito C., Costa J., Carrondo M.J.T., and Alves P.M. Novel culture strategy for human stem cell expansion and neuronal differentiation. J. Neurosc. Res. 85 16 (2007) 3557-3566
19. Ulloa-Montoya F., Verfaillie C.M., and Hu W.S. Culture systems for pluripotent stem cells. J. Biosci. Bioeng. 100 (2005) 12-27
20. Youn B.S., Sen A., Behie L.A., Girgis-Gabardo A., and Hassell J.A. Scale-up of breast cancer stem cell aggregate cultures to suspension bioreactors. Biotechnol. Prog. 22 (2006) 801-810
21. zur Nieden N.I., Cormier J.T., Rancourt D.E., and Kallos M.S. Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactors. J. Biotechnol. 129 3 (2007) 421-432