An evaluation of the in vitro cytotoxicities of 50 chemicals by using an electrical current exclusion method versus the neutral red uptake and MTT assays

Alternatives to Laboratory Animals

Volumn 33, Issue 6, 2005, Pages 591-601

  Lindl, Toni ^a   Lewandowski, Birgit ^a   Schreyögg, Sonya ^a   Stäudte, Andrea ^a  

^a Institute for Applied Cell Culture   (Germany)

Author keywords

Alternatives to animal testing; CASY model TTC; Cell counting; Cell proliferation; Cell viability; Cytotoxicity; Electrical current exclusion; MTT; Neutral red uptake; Trypan blue exclusion

Indexed keywords

ACETONITRILE; ACETYLSALICYLIC ACID; ACRYLAMIDE; ALCOHOL; ANILINE; CALCIUM CHLORIDE; CYCLOPHOSPHAMIDE; DIMETHYL SULFOXIDE; FERROUS SULFATE; FLUORIDE SODIUM; FORMAZAN; GLYCEROL; IMIDAZOLE; ISONIAZID; LITHIUM CHLORIDE; MAGNESIUM CHLORIDE; METHANOL; N,N DIMETHYLFORMAMIDE; NEUTRAL RED; PHENAZONE; POTASSIUM FERRICYANIDE; PROPANOL; PYRIDINE; SALICYLIC ACID; SODIUM CHLORIDE; SODIUM SULFITE; THEOPHYLLINE; THIOACETAMIDE; TRYPAN BLUE; UNINDEXED DRUG;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL DEATH; CELL MEMBRANE; CELL ORGANELLE; CELL PROLIFERATION; CELL VIABILITY; CELL VOLUME; CYTOTOXICITY; ELECTRIC CURRENT; IC 50; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA;

EID: 31444439892     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290503300614     Document Type: Article

References (29)

1. Halle, W. (1998). Toxizitätsprüfungen in Zellkulturen für eine Vorhersage der akuten Toxizität (LD50) zur Einsparung von Tierversuchen. 94pp.
2. Schriften des Forschungszentrum Jülich, Life Sciences, Vol. 1, 93pp. Jülich, Germany: Forschungszentrum.
3. Now also available in English: ATLA 31, 89-198, 2003.
4. Halle, W. & Göres, E. (1988). Register der Zytotoxizität (IC50) in der Zellkultur und Möglichkeiten zur Abschätzung der akuten Toxizität (LD50). In Beiträge zur Wirkstoffforschung (ed. P. Oehme, H. Löwe & E. Göres), Vol. 32, 108pp. Berlin, Germany: Institut für Wirkstoffforschung.
5. Bondesson, I., Ekwall, B., Hellberg, S., Romert, L., Stenberg, K. & Walum, E. (1989). MEIC - A new international multicenter study to evaluate the relevance to human toxicity data on in vitro cytotoxicity tests. Cell Biology and Toxicology 5, 331-347.
6. ICCVAM (2001). Guidance document on using in vitro data to estimate in vivo starting doses for acute toxicity, based on recommendations from an International Workshop organized by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and the National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). 102pp. NIH Publication No: 01-4500. Research Triangle Park, NC, USA: ICCVAM.
7. Borenfreund, E. & Puerner, J.A. (1985). Toxicity determination in vitro by morphological alterations and neutral red absorption. Toxicology Letters 24, 119-124.
8. Spielmann, H., Liebsch, M., Kalweit, S., Moldenhauer, F., Wirnsberger, T., Holzhütter, H.G., Schneider, B., Glaser, S., Gerner, I., Pape, W.J.W., Kreiling, R., Krauser, K., Miltenburger, H.G., Steiling, W., Luepke, N.P., Müller, N., Kreuzer, H., Mürmann, P., Spengler, J., Bertram-Neis, E., Siegemund, B. & Wiebel, F.J. (1996). Results of a validation study in Germany on two in vitro alternatives to the Draize eye irritation test, the HET-CAM test and the 3T3 NRU cytotoxicity test. ATLA 24, 741-858.
9. Lindl, T. (2002). Zell- und Gewebekultur. 5th edition, 316pp. Heidelberg, Germany: Spektrum Akademischer Verlag.
10. Freshney, R.I. (2000). Culture of Animal Cells. 4th edition, 600pp. New York, NY, USA: Wiley-Liss.
11. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 207-212.
12. Ishiyama, H., Tominaga, H., Shiga, M., Sasamoto, K., Ohkura, Y. & Ueno, K. (1996). A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, Neutral Red and Crystal Violet. Biological Pharmaceutical Bulletin 19, 518-520.
13. Sousa, M.C. & Poiares-da-Silva, J. (2001). The cytotoxic effects of ciprofloxacin in Giardia lamblia trophozoites. Toxicology in Vitro 15, 297-301.
14. Winkelmeier, P., Glauner, B. & Lindl, T. (1993). Quantification of cytotoxicity by cell volume and cell proliferation. ATLA 21, 269-280.
15. Li, N., Miao, X., Takakuwa, M., Sato, K. & Sato, M. (1999). Effect of dental material HEMA monomer on human dental pulp cells. Artificial Cells Blood Substitutes Immobilization Biotechnology 27, 85-90.
16. Yoshii, E. (1996). Cytotoxic effects of acrylates and methacrylates: Relationship of monomer structures and cytotoxicity. Journal of Biomedical Material Research 37, 517-524.
17. Reichl, F.X., Walther, U.I., Druner, J., Kehe, K., Hickel, R., Kunzelmann, K.H., Spahl, W., Hume, W.R., Benschop, H. & Forth, W. (2001). Cytotoxicity of dental composite components and mercury compounds in lung cells. Dental Materials 17, 95-101.
18. Pomerat, C.M. & Leake, C.D. (1954). Short term cultures for drug assays: general considerations. Annals of the New York Academy of Sciences 58, 1110-1128.
19. Vancha, A.R., Govindaraju, S., Parsa, K.V., Jasti, M., Gonzalez-Garcia, M. & Ballestero, R.P. (2004). Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer. Biomed Central (BMC) Biotechnology 4, 23.
20. Smith, C.G., Grady, J.E. & Northam, J.I. (1963). Relationship between cytotoxicity in vitro and whole animal toxicity. Cancer Chemotherapeutic Report 30, 9-12.
21. Halle, W., Spielmann, H. & Liebsch, M. (2000). Zur Prädiktion der lethalen Konzentration beim Menschen mit Hilfe von Zytotoxizitätsdaten von 50 MEIC-Stoffen. Alternativen zu Tierexperimenten (ALTEX) 17, 75-79.
22. Philips, J.C., Gibson, W.B., Yam, J., Alden, C.L. & Hard, G.C. (1990). Survey of the QSAR and in vitro approaches for developing non-animal methods to supersede the in vivo LD50 test. Food and Chemical Toxicology 28, 375-394.
23. Garle, M., Fentem, J. & Fry, J.R. (1994). In vitro cytotoxicity tests for the prediction of acute toxicity in vivo. Toxicology in Vitro 8, 1303-1312.
24. Borenfreund, E., Babich, H. & Martin-Alguacil, N. (1990). Comparison of two in vitro cytotoxicity assays: the neutral red (NR) and tetrazolium MTT tests. Toxicology in Vitro 2, 1-6.
25. Krause, A.W., Carley, W.W. & Webb, W.W. (1984). Fluorescent erythrosine B is preferable to trypan blue as a vital exclusion dye for mammalian cells in monolayer culture. Journal of Histochemistry and Cytochemistry 32, 1084-1090.
26. Wilhelm, F., Melzig, M., Görscher, T. & Franke, G. (1995). Die unterschiedliche Aussagekraft verschiedener Vitalfärbungen des Hornhautepithels. Ophtalmologe 92, 496-498.
27. Gain, P., Thuret, G., Chiquet, C., Dumollard, J.M., Mosnier, J.F., Burillon, C., Delbosc, B., Herve, P. & Campos, L. (2002). Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique. British Journal of Ophthalmology 86, 306-310.
28. Maile, W., Lindl, T. & Weiss, D.G. (1989). New methods for cytotoxicity testing: quantitative video microscopy of intracellular motion and mitochondria-specific fluorescence. Journal of Molecular Toxicology 1, 427-437.
29. George, T.C., Basiji, D.A., Hall, B.E., Lynch, D.H., Ortyn, W.E., Perry, D.J., Seo, M.J., Zimmermann, C.A. & Morrissey, P.J. (2004). Distinguishing modes of cell death using the ImageStream multispectral imaging flow cytometer. Cytometry A 59, 237-45.