A simple and effective strategy for solving the problem of inclusion bodies in recombinant protein technology: His-tag deletions enhance soluble expression

Applied Microbiology and Biotechnology

Volumn 97, Issue 2, 2013, Pages 837-845

  Zhu, Shaozhou ^a   Gong, Cuiyu ^a   Ren, Lu ^a   Li, Xingzhou ^a,b   Song, Dawei ^a   Zheng, Guojun ^a  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

^b BEIJING INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (China)

Author keywords

(+) Lactamase; ( ) Lactamase; His tag; Inclusion bodies; Uridine phosphorylase

Indexed keywords

AEROPYRUM PERNIX; BRADYRHIZOBIUM JAPONICUM; E. COLI; FUSION PARTNERS; FUSION TAG; HETEROLOGOUS PROTEINS; HIS-TAG; INCLUSION BODIES; INTENSIVE RESEARCH; KEY FACTORS; LACTAMASES; MICROBIAL CELL FACTORIES; PET SYSTEMS; POWERFUL SYSTEMS; PROCESS NEEDS; PRODUCTION OF; PROTEIN EXPRESSIONS; SOLUBLE EXPRESSION; STRUCTURE-BASED; TARGET PROTEINS; URIDINE PHOSPHORYLASE;

AMINO ACIDS; ESCHERICHIA COLI; INDUSTRIAL APPLICATIONS; INDUSTRIAL RESEARCH; PHOSPHORYLATION; PURIFICATION;

RECOMBINANT PROTEINS;

PENICILLINASE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN; URIDINE PHOSPHORYLASE;

BIOTECHNOLOGY; CATALYST; COLIFORM BACTERIUM; ENZYME ACTIVITY; EUKARYOTE; GENE EXPRESSION; PROTEIN; RECOMBINATION;

AEROPYRUM PERNIX; AMINO TERMINAL SEQUENCE; ARTICLE; BIOCATALYST; BRADYRHIZOBIUM JAPONICUM; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION;

AMIDOHYDROLASES; BIOTECHNOLOGY; GENETIC VECTORS; HISTIDINE; INCLUSION BODIES; PLASMIDS; RECOMBINANT PROTEINS; URIDINE PHOSPHORYLASE;

AEROPYRUM PERNIX; BRADYRHIZOBIUM JAPONICUM; ESCHERICHIA COLI;

EID: 84873996574     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4630-y     Document Type: Article

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