Effects of co-expression of molecular chaperones on heterologous soluble expression of the cold-active lipase Lip-948

Protein Expression and Purification

Volumn 77, Issue 2, 2011, Pages 166-172

  Shuo Shuo, Cui ^a,b   Xue Zheng, Lin ^a,b   Ji Hong, Shen ^a,b  

^a FIRST INSTITUTE OF OCEANOGRAPHY   (China)

^b Chongqing Institute of Geology and Mineral Resources   (China)

Author keywords

Co expression; Cold active lipase; Molecular chaperone; Soluble expression

Indexed keywords

4 NITROPHENYL; 4-NITROPHENYL; BACTERIAL PROTEIN; CHAPERONE; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; NITROPHENOL; OLIGOPEPTIDE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AFFINITY CHROMATOGRAPHY; ANTARCTICA; ARTICLE; CHEMISTRY; COLD; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSYCHROBACTER; SOLUBILITY; TEMPERATURE;

ANTARCTIC REGIONS; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; COLD TEMPERATURE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION; HISTIDINE; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; MOLECULAR CHAPERONES; NITROPHENOLS; OLIGOPEPTIDES; PLASMIDS; PSYCHROBACTER; RECOMBINANT PROTEINS; SOLUBILITY; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSYCHROBACTER; PSYCHROBACTER SP.;

EID: 79952623795     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.01.009     Document Type: Article

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