Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones

Applied Microbiology and Biotechnology

Volumn 81, Issue 4, 2008, Pages 721-729

  Wardenga, Rainer ^a   Hollmann, Frank ^b,c   Thum, Oliver ^b   Bornscheuer, Uwe ^a  

^a Institute of Biochemistry   (Germany)

^b EVONIK INDUSTRIES AG   (Germany)

^c DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Chaperones; Codon usage; Inclusion body; N acyl l aminoacylase; Soluble expression; StrepTag; Synthetic gene

Indexed keywords

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; NUCLEIC ACIDS; PROTEINS; PURIFICATION;

CHAPERONES; CODON USAGE; INCLUSION BODY; N-ACYL-L-AMINOACYLASE; SOLUBLE EXPRESSION; STREPTAG; SYNTHETIC GENE;

ESCHERICHIA COLI;

AMINOACYLASE; CHAPERONE; SYNTHETIC DNA;

CHROMATOGRAPHY; COLIFORM BACTERIUM; COMPARATIVE STUDY; ENZYME ACTIVITY; GENE EXPRESSION; OPTIMIZATION; PIG; PROTEIN; RECOMBINATION;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINOACYLASE 1 GENE; ANIMAL EXPERIMENT; ARTICLE; CODON; ESCHERICHIA COLI; GENE; GENE EXPRESSION; KIDNEY; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SWINE;

AMIDOHYDROLASES; ANIMALS; CLONING, MOLECULAR; CODON; ESCHERICHIA COLI; GENE EXPRESSION; GENES, SYNTHETIC; GENETIC VECTORS; MOLECULAR CHAPERONES; MOLECULAR WEIGHT; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SWINE;

ESCHERICHIA COLI; PROKARYOTA; SUIDAE; SUS;

EID: 57249095871     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1716-7     Document Type: Article

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