Recombinant glutamine synthetase (GS) from C. glutamicum existed as both hexamers & dedocamers and C-terminal His-tag enhanced inclusion bodies formation in E. coli

Applied Biochemistry and Biotechnology

Volumn 159, Issue 3, 2009, Pages 614-622

  Li, Ye ^a   Yang, Guang ^a   Huang, Xing ^a   Ye, Bo ^b   Liu, Ming ^a   Lin, Zhanglin ^a   Li, Chun ^b   Cao, Zhu An ^a  

^a TSINGHUA UNIVERSITY   (China)

^b BEIJING INSTITUTE OF TECHNOLOGY   (China)

Author keywords

Corynebacterium glutamicum; Glutamine synthetase; His tag; Inclusion bodies; Quaternary structure

Indexed keywords

CORYNEBACTERIUM GLUTAMICUM; GLUTAMINE SYNTHETASE; HIS-TAG; INCLUSION BODIES; QUATERNARY STRUCTURE;

AMINO ACIDS; ESCHERICHIA COLI;

CRYSTAL STRUCTURE;

GLUTAMATE AMMONIA LIGASE; POLYHISTIDINE TAG; RECOMBINANT ENZYME;

BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOMASS FERMENTATION; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CONFERENCE PAPER; CONFORMATION; CORYNEBACTERIUM GLUTAMICUM; CRYSTAL STRUCTURE; ENZYME ASSAY; ESCHERICHIA COLI; GEL FILTRATION; GEL PERMEATION CHROMATOGRAPHY; GENE AMPLIFICATION; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; RIBOSOME;

CHROMATOGRAPHY, GEL; CORYNEBACTERIUM GLUTAMICUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTAMATE-AMMONIA LIGASE; INCLUSION BODIES; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS;

CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI;

EID: 73149103477     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8493-8     Document Type: Conference Paper

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