Loop-mediated isothermal amplification assay targeting the femA gene for rapid detection of Staphylococcus aureus from clinical and food samples

Journal of Microbiology and Biotechnology

Volumn 23, Issue 2, 2013, Pages 246-250

  Zhao, Xihong ^a,b,c   Li, Yanmei ^d,e   Park, Myoungsu ^c   Wang, Jun ^c   Zhang, Youhong ^a   He, Xiaowei ^b   Forghani, Fereidoun ^c   Wang, Li ^f   Yu, Guangchao ^g   Oh, Deog Hwan ^c  

^a WUHAN INSTITUTE OF TECHNOLOGY   (China)

^b SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^c Kangwon National University   (South Korea)

^d GUANGZHOU WOMEN AND CHILDREN S MEDICAL CENTER   (China)

^e UNIVERSITY OF MARYLAND DENTAL SCHOOL   (United States)

^f SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^g THE FIRST AFFILIATED HOSPITAL OF JINAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; BLOOD SAMPLING; COLONY FORMING UNIT; COOKED FOOD; COST EFFECTIVENESS ANALYSIS; DNA DETERMINATION; FECES; FEMA GENE; FOOD ANALYSIS; GENE SEQUENCE; GENE TARGETING; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MILK; NONHUMAN; NOSE SMEAR; POLYMERASE CHAIN REACTION; PUS; RAW MEAT; SENSITIVITY AND SPECIFICITY; SPUTUM; STAPHYLOCOCCUS AUREUS; STRAIN DIFFERENCE; URINE;

EID: 84873823454     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1207.07022     Document Type: Article

References (22)

1. Alarcon, B., B. Vicedo, and R. Aznar. 2006. PCR-based procedures for detection and quantification of Staphylococcus aureus and their application in food. J. Appl. Microbiol. 100: 352-364.
2. Goto, M., H. Hayashidani, K. Takatori, and Y. Hara-Kudo. 2007. Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop-mediated isothermal amplification assay. Lett. Appl. Microbiol. 45: 100-107.
3. Hein, I., A. Lehner, P. Rieck, K. Klein, E. Brandl, and M. Wagner. 2001. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl. Environ. Microbiol. 67: 3122-3126.
4. Johnson, S., D. Krüger, and H. Labischinski. 1995. FemA of Staphylococcus aureus: Isolation and immunodetection. FEMS. Microbiol. Lett. 132: 221-218.
5. Margrit, S., S.-S. Ursula, and W. Schmidt-Lorenz. 1990. Temperature limits of growth, TNase and enterotoxin production of Staphylococcus aureus strains isolated from foods. Int. J. Food Microbiol. 11: 1-19.
6. Mori, Y., K. Nagamine, N. Tomita, and T. Notomi. 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289: 150-154.
7. Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, and T. Hase. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28: E63.
8. Palomares, C., M. J. Torres, A. Torres, J. Aznar, and J. C. Palomares. 2003. Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn. Microbiol. Infect. Dis. 45: 183-189.
9. Shi, X. M., F. Long, and B. Suo. 2010. Molecular methods for the detection and characterization of foodborne pathogens. Pure Appl. Chem. 82: 69-79.
10. Suwanampai, T., K. Pattaragulvanit, P. Pattanamahakul, O. Sutheinkul, K. Okada, T. Honda, and J. Thaniyavarn. 2011. Evaluation of loop-mediated isothermal amplification method for detecting enterotoxin A gene of Staphylococcus aureus in pork. SE. Asian J. Trop. Med. 42: 1489-1497.
11. Xu, Z. B., L. Shi, C. Zhang, L. Zhang, X. Li, Y. Cao, L. Li, and S. Yamasaki. 2007. Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China. Clin. Microbiol. Infect. 13: 980-984.
12. Xu, Z. B., L. Shi, M. J. Alam, L. Li, and S. Yamasaki. 2008. Integron-bearing methicillin-resistant coagulase-negative staphylococci in South China, 2001-2004. FEMS Microbiol. Lett. 278: 223-230.
13. Xu, Z. B., L. Li, M. J. Alam, S. Yamasaki, and L. Shi. 2008. First confirmation of integron-bearing methicillin-resistant Staphylococcus aureus. Curr. Microbiol. 57: 264-268.
14. Xu, Z. B., L. Li, M. E. Shirtliff, M. J. Alam, S. Yamasaki, and L. Shi. 2009. Occurrence and characteristics of class 1 and 2 integrons in Pseudomonas aeruginosa isolates from patients in Southern China. J. Clin. Microbiol. 47: 230-234.
15. Xu, Z. B., L. Li, M. E. Shirtliff, B. M. Peters, Y. Peng, M. J. Alam, S. Yamasaki, and L. Shi. 2010. First report of class 2 integron in clinical Enterococcus faecalis and class 1 integron in Enterococcus faecium in South China. Diag. Microbiol. Infect. Dis. 68: 315-317.
16. Xu, Z. B., L. Li, M. E. Shirtliff, B. M. Peters, B. Li, Y. Peng, M. J. Alam, S. Yamasaki, and L. Shi. 2011. Resistance class 1 integron in clinical methicillin-resistant Staphylococcus aureus strains in southern China, 2001-2006. Clin. Microbiol. Infect. 17: 714-718.
17. Xu, Z. B., L. Li, L. Shi, and M. E. Shirtliff. 2011. Class 1 integron in staphylococci. Mol. Biol. Rep. 38: 5261-5279.
18. Xu, Z. B., L. Li, J. Chu, B. Peters, M. Harris, B. Li, B. Shi, and M. Shirtliff. 2012. Development and application of loopmediated isothermal amplification assays on rapid detection of various types of staphylococci strains. Food Res. Int. 47: 166-173
19. Yang, H., X. Y. Ma, X. Z. Zhang, Y. Wang, and W. Zhang. 2011. Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus in food. Eur. Food Res. Technol. 232: 769-776.
20. Zhao, X. H., Y. M. Li, L. Wang, L. J. You, Z. B. Xu, L. Li, et al. 2010. Development and application of a loop-mediated isothermal amplification method on rapid detection of Escherichia coli O157 strains from food samples. Mol. Biol. Rep. 37: 2183-2188.
21. Zhao, X. H., L. Wang, J. Chu, Y. M. Li, Y. Y. Li, Z. B. Xu, et al. 2010. Development and Application of a rapid and simple loop-mediated isothermal amplification method for food-borne Salmonella detection. Food Sci. Biotechnol. 19: 1655-1659.
22. Zhao, X. H., L. Wang, J. Chu, Y. Y. Li, Y. M. Li, Z. B. Xu, et al. 2010. Rapid detection of Vibrio parahaemolyticus strains and virulent factors by loop-mediated isothermal amplification assays. Food Sci. Biotechnol. 19: 1191-1197.