Class 1 integron in staphylococci

Molecular Biology Reports

Volumn 38, Issue 8, 2011, Pages 5261-5279

  Xu, Zhenbo ^a,b   Li, Lin ^a   Shi, Lei ^a,c   Shirtliff, Mark E ^b,d  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b UNIVERSITY OF MARYLAND DENTAL SCHOOL   (United States)

^c Osaka Prefecture University   (Japan)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Antibiotic resistance; Class 1 integron; Methicillin resistance staphylococci (MRS); Mobile genetic element; SCCmec

Indexed keywords

AMOXICILLIN PLUS CLAVULANIC ACID; CHLORAMPHENICOL; CIPROFLOXACIN; CLINDAMYCIN; COTRIMOXAZOLE; ERYTHROMYCIN; GENTAMICIN; LEVOFLOXACIN; OXACILLIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CLASS 1 INTEGRON; COPY NUMBER VARIATION; DNA SEQUENCE; GENE CASSETTE; GENETIC ASSOCIATION; HOUSEKEEPING GENE; INTEGRON; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR DYNAMICS; MOLECULAR EPIDEMIOLOGY; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; VALIDATION PROCESS;

EVOLUTION, MOLECULAR; HUMANS; INTEGRONS; METHICILLIN RESISTANCE; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS;

NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS;

EID: 84855211393     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-0676-7     Document Type: Article

References (103)

1. Ahmed AM, Nakano H, Shimamoto T (2005) Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. J Antimicrob Chemother 55:371-374 (Pubitemid 40430691)
2. Arakawa Y, Murakami M, Suzuki K, Ito H, Wacharotayankun R, Ohsuka S, Kato N, Ohta M (1995) A novel integron-like element carrying the metallo-b-lactamase gene blaIMP. Anti-microb Agents Chemother 39:1612-1615
3. Barker A, Clark CA, Manning PA (1004) Identification of VCR, a repeated sequence associated with a locus encoding a hem-agglutinin in Vibrio cholerae O1. J Bacteriol 176:5450-5458
4. Barlow RS, Pemberton JM, Desmarchelier PM, Gobius KS (2004) Isolation and characterization of integron-containing bacteria without antibiotic selection. Antimicrob Agents Che-mother 48:838-842 (Pubitemid 38280333)
5. Ben-Ami R, Navon-Venezia S, Chwartz D, Carmeli Y (2003) Infection of a ventriculoatrial shunt with phenotypically variable Staphylococcus epidermidis masquerading as polymicrobial bacteremia due to various coagulase-negative staphylococci and Kocuria varians. J Clin Microbiol 41:2444-2447 (Pubitemid 36712449)
6. Boucher Y, Labbate M, Koenig JE, Stokes HW (2007) Integ-rons: mobilizable platforms that promote genetic diversity in bacteria. Trends Microbiol 15:301-309 (Pubitemid 47017712)
7. Centers for Disease Control and Prevention (1972) Outline for surveillance and control of nosocomial infections, revised. U.S. Department of Health, Education and Welfare, Public Health Service
8. Centers for Disease Control and Prevention (2003) Methicillin-resistant Staphylococcus aureus infections among competitive sports participants-Colorado, Indiana, Pennsylvania, and Los Angeles County, 2000-2003. MMWR Morb Mortal Wkly Rep 52:793-795
9. Centron D, Roy PH (2002) Presence of a group II intron in a multiresistant Serratia marcescens strain that harbors three in-tegrons and a novel gene fusion. Antimicrob Agents Chemother 46:1402-1409 (Pubitemid 34415321)
10. Chambers HF (2001) The changing epidemiology of Staphylo-coccus aureus? Emerg Infect Dis 7:178-182
11. Chang S, Sun C, Yang L, Luh K, Hsieh W (1997) Increasing nosocomial infections of methicillin-resistant Staphylococcus aureus at a teaching hospital in Taiwan. Int J Antimicrob Agents 8:109-114 (Pubitemid 27191839)
12. Clark NC, Olsvik Ø, Swenson JM, Spiegel CA, Tenover FC (1999) Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob Agents Chemother 43:157-160 (Pubitemid 29069216)
13. Clark CA, Purins L, Kaewrakon P, Focareta T, Manning PA (2000) The Vibrio cholerae O1 chromosomal integron. Microbiology 146:2605-2612
14. Clinical and Laboratory Standards Institute (2005) Performance standards for antimicrobial susceptibility testing: 15th informational supplement CLSI/NCCLS document M100-S15. Clinical and Laboratory Standards Institute, Wayne
15. Collis CM, Hall RM (1995) Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob Agents Chemother 39:155-162
16. Collis CM, Grammaticopoulos G, Briton J, Stokes HW, Hall RM (1993) Site-specific insertion of gene cassettes into integ-rons. Mol Microbiol 9:41-52 (Pubitemid 23217736)
17. Collis CM, Kim MJ, Stokes HW, Hall RM (1998) Binding of the purified integron DNA integrase Intl1 to integron-and cassette-associated recombination sites. Mol Microbiol 29:477-490 (Pubitemid 28330782)
18. Correia M, Boavida F, Grosso F, Salgado MJ, Lito ML, Cristino JM, Mendo S, Duarte A (2003) Molecular characterization of a new class 3 integron in Klebsiella pneumoniae. Antimicrob Agents Chemother 47:2838-2843 (Pubitemid 37040256)
19. Crowley D, Daly M, Lucey B (2002) Molecular epidemiology of cystic fibrosis-linked Burkholderia cepacia complex isolates from three national referral centres in Ireland. J Appl Microbiol 92:992-1004 (Pubitemid 34429722)
20. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38:1008-1015 (Pubitemid 30140551)
21. Falbo V, Carattoli A, Tosini F, Pezzella C, Dionisi AM, Luzzi I (1999) Antibiotic resistance conferred by a conjugative plasmid and a class I integron in Vibrio cholerae O1 El or strains isolated in Albania and Italy. Antimicrob Agents Chemother 43:693-696 (Pubitemid 29109544)
22. Fluit AC, Schmitz FJ (1999) Class 1 integrons, gene cassettes, mobility, and epidemiology. Eur J Clin Microbiol Infect Dis 18:761-770
23. Fluit AC, Schmitz FJ (2004) Resistance integrons and super-integrons. Clin Microbiol Infect 10:272-288 (Pubitemid 38519353)
24. Francia MV, Zabala JC, de la Cruz F, Garcia Lobo JM (1999) The IntI1 integron integrase preferentially binds singlestranded DNA of the attC site. J Bacteriol 181:6844-6849 (Pubitemid 29518004)
25. Gibreel A, Skold O (2000) An integron cassette carrying dfr1 with 90-bp repeat sequences located on the chromosome of trimethoprim-resistant isolates of Campylobacter jejuni. Microb Drug Resist 6:91-98
26. Gillet Y, Issartel B, Vanhems P, Fournet JC, Lina G, Bes M (2002) Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759 (Pubitemid 34215075)
27. Girlich D, Karim A, Poirel L, Cavin MH, Verny C, Nordmann P (2000) Molecular epidemiology of an outbreak due to IRT-2 beta-lactamase-producing strains of Klebsiella pneumoniae in a geriatric department. J Antimicrob Chemother 45:467-473 (Pubitemid 30199139)
28. Gu B, Tong M, Zhao W, Liu G, Ning M, Pan S, Zhao W (2007) Prevalence and characterization of class 1 integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing, China. J Clin Microbiol 45:241-243 (Pubitemid 46106436)
29. Hall RM, Collis CM (1995) Mobile gene cassettes and integ-rons: capture and spread of genes by site-specific recombination. Mol Microbiol 15:593-600
30. Hall RM, Collis CM (1998) Antibiotic resistance in gram-negative bacteria: the role of gene cassettes and integrons. Drug Res Updates 1:109-119
31. Hall RM, Stokes HW (1993) Integrons: novel DNA elements which capture genes by site-specific recombination. Genetica 90:115-132 (Pubitemid 2032213)
32. Hall RM, Brown HJ, Brookes DE, Stokes HW (1994) Integrons found in different locations have identical 5b ends but variable 3b ends. J Bacteriol 176:6286-6294 (Pubitemid 24313809)
33. Hall RM, Collis CM, Kim MJ, Partridge SR, Recchia GD, Stokes HW (1999) Mobile gene cassettes and integrons in evolution. Ann N Y Acad Sci 870:68-80 (Pubitemid 29349330)
34. Hanssen A, Kjeldsen G, Sollid JUE (2004) Local variants of staphylococcal cassette chromosome mec in sporadic methicil-lin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci: evidence of horizontal gene transfer? Antimicrob Agents Chemother 48:285-296 (Pubitemid 38040210)
35. Hansson K, Sundstrom L, Pelletier A, Roy PH (2002) IntI2 integron integrase in Tn7. J Bacteriol 184:1712-1721 (Pubitemid 34188223)
36. Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ (2000) DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406:477-483 (Pubitemid 30625724)
37. Hisata K, Kuwahara-Arai K, Yamanoto M, Ito T, Nakatomi Y, Cui L (2005) Dissemination of methicillin-resistant staphylo-cocci among healthy Japanese children. J Clin Microbiol 43: 3364-3372 (Pubitemid 40981349)
38. Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C, Hiramatsu K (2001) Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococ-cus aureus. Antimicrob Agents Chemother 45:1323-1336 (Pubitemid 32374098)
39. Katayama Y, Ito T, Hiramatsu K (2000) A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 44:1549-1555 (Pubitemid 30340793)
40. Koeleman JGM, Stoof J, Van Der Bijl MW, Vandenbroucke-Grauls CMJE, Savelkoul PHM (2001) Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 39:8-13 (Pubitemid 32059560)
41. Kuroda MT, Ohta I, Uchiyama T, Baba H, Yuzawa I, Kobayashi L, Cui A, Oguchi K, Aoki YN, Lian J, Ito T, Kanamori M, Matsumaru H, Maruyama A, Murakami H, Hosoyama A, Mizutani-Ui Y, Takahashi NK, Sawano T, Inoue R, Kaito C, Sekimizu K, Hirakawa H, Kuhara S, Goto S, Yabuzaki J, Kanehisa M, Yamashita A, Oshima K, Furuya K, Yoshino C, Shiba T, Hattori M, Ogasawara N, Hayashi H, Hiramatsu K (2001) Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357:1225-1240 (Pubitemid 32539050)
42. L'Abee-Lund TM, Sorum H (2001) Class 1 integrons mediate antibiotic resistance in the fish pathogen Aeromonas salmoni-cida worldwide. Microb Drug Resist 7:263-272 (Pubitemid 33104601)
43. Labbate M, Case RJ, Stokes HW (2009) The integron/gene cassette system: an active player in bacterial adaptation. In: Gogarten M. Gogarten P, Olendzenski L (eds) Horizontal gene transfer. Genomes in flux, vol 532, chap 6. Humana Press, New York
44. Liebert CA, Hall RM, Summers AO (1999) Transposon Tn21, flagship of the floating genome. Microbiol Mol Biol Rev 63:507-522 (Pubitemid 29422780)
45. Maguire AJ, Brown DFJ, Gray JJ, Desselberger U (2001) Rapid screening technique for class 1 integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology. Antimicrob Agents Chemother 45: 1022-1029 (Pubitemid 32230978)
46. Martin C, Timm J, Rauzier J, Gomez-Lus R, Davies J, Giequel B (1990) Transposition of an antibiotic resistance element in mycobacteria. Nature 345:739-743
47. Martinez-Freijo P, Fluit AC, Schmitz FJ, Grek VSC, Verhoef J, Jones ME (1998) Class 1 integrons in gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds. J Antimicrob Chemother 42:689-696 (Pubitemid 29008068)
48. Mazel D (2006) Integrons: agents of bacterial evolution. Nat Rev Microbiol 4:608-620 (Pubitemid 44317023)
49. Mazel D, Dychinco B, Webb VA, Davies J (1998) A distinctive class of integron in the Vibrio cholerae genome. Science 280:605-608 (Pubitemid 28227767)
50. Mazel D, Dychinco B, Webb VA, Davies J (2000) Antibiotic resistance in the ECOR collection: integrons and identification of a novel aad gene. Antimicrob Agents Chemother 44:1568-1574 (Pubitemid 30340796)
51. McIver CJ, White PA, Jones LA (2002) Epidemic strains of Shigella sonnei biotype g carrying integrons. J Clin Microbiol 40:1538-1540 (Pubitemid 34279935)
52. Mehrotra M, Wang G, Johnson W (2000) Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, ex-foliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 38:1032-1035 (Pubitemid 30140554)
53. Mulazimoglu L, Drenning SD, Muder BR (1996) Vancomycin-gentamicin synergism revisited: effect of gentamicin susceptibility of methicillin- resistant Staphylococcus aureus. Antimic-rob Agents Chemother 40:1534-1535 (Pubitemid 26169063)
54. Naas T, Sougakoff W, Casetta A, Nordmann P (1998) Molecular characterization of OXA-20, a novel class D beta-lactamase, and its integron from Pseudomonas aeruginosa. Antimicrob Agents Chemother 42:2074-2083 (Pubitemid 28363096)
55. Naimi TS, LeDell KH, Como-Sabetti K, Borchardt SM, Boxrud DJ, Etienne J (2003) Comparison of community-and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA 290:2976-2984 (Pubitemid 37505530)
56. Nakatomi Y, Sugiyama J (1998) A rapid latex agglutination assay for the detection of penicillin-binding protein 2'. Micro-biol Immunol 42:739-743 (Pubitemid 28547596)
57. Nandi S, Maurer JJ, Hofacre C, Summers AO (2004) Gram-positive bacteria are a major reservoir of class 1 antibiotic resistance integrons in poultry litter. Proc Natl Acad Sci USA 101:7118-7122 (Pubitemid 38596452)
58. Nemergut DR, Robeson MS, Kysela RF, Martin AP, Schmidt SK, Knight R (2008) Insights and inferences about integron evolution from genomic data. BMC Genomics 9:1-12
59. Nesvera J, Hochmannova J, Patek M (1998) An integron of class 1 is present on the plasmid pCG4 from gram-positive bacterium Corynebacterium glutamicum. FEMS Microbiol Lett 169: 391-395 (Pubitemid 29050806)
60. Nield BS, Holmes AJ, Gillings MR, Recchia GD, Mabbutt BC, Nevalainen KM, Stokes HW (2001) Recovery of new integron classes from environmental DNA. FEMS Microbiol Lett 195:59-65 (Pubitemid 32146536)
61. Nordmann P, Poirel L (2002) Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect 8:321-331 (Pubitemid 34993804)
62. Norskov-Lauritsen N, Sandvang D, Hedegaard J (2001) Clonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county. J Med Microbiol 50:636-641 (Pubitemid 32588159)
63. Nouwen JL, Van Belkum A, De Marie S, Sluijs J, Wielenga JJ, Kluytmans JW, Verbrugh HA (1998) Clonal rxpansion of Staphylococcus epidermidis strains causing hickman catheter-related infections in a hemato-oncologic department. J Clin Microbiol 36:2696-2702 (Pubitemid 28380979)
64. Obayashi Y, Fujita J, Ichiyama S, Hojo S, Negayama K, Takashima C, Miyawaki H, Tanabe T, Yamaji Y, Kawanishi K, Takahara J (1997) Investigation of nosocomial infection caused by arbekacin-resistant, methicillin-resistant. Diagn Microbiol Infect Dis 28:53-59 (Pubitemid 27323785)
65. Oliveira DC, Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Anti-microb Agents Chemother 46:2155-2161 (Pubitemid 34665910)
66. Paulsen IT, Littlejohn TG, Radstrom P, Sundstrom L, Skold O, Swedberg G, Skurray RA (1993) The 3b conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants. Antimicrob Agents Chemother 37:761-768 (Pubitemid 23096875)
67. Ramirez MS, Vargas LJ, Cagnoni V, Tokumoto M, Centron D (2005) Class 2 integron with a novel cassette array in a Burk-holderia cenocepacia isolate. Antimicrob Agents Chemother 49:4418-4420 (Pubitemid 41401004)
68. Ramirez MS, Quiroga C, Centron D (2005) Novel rearrangement of a class 2 integron in two non-epidemiologically related isolates of Acinetobacter baumannii. Antimicrob Agents Che-mother 49:5179-5181 (Pubitemid 41778947)
69. Recchia GD, Hall RM (1995) Gene cassettes, a new class of mobile element. Microbiology 141:3015-3027 (Pubitemid 26011340)
70. Recchia GD, Hall RM (1997) Origins of the mobile gene cassettes found in integrons. Trends Microbiol 5:389-394 (Pubitemid 27417824)
71. Ridley A, Threlfall EJ (1998) Molecular epidemiology of antibiotic resistance genes in multiresistant epidemic Salmonella typhimurium DT 104. Microb Drug Resist 4:113-118 (Pubitemid 28275281)
72. Rowe-Magnus DA, Mazel D (2001) Integrons: natural tools for bacterial genome evolution. Curr Opin Microbiol 4:565-569 (Pubitemid 32964106)
73. Rowe-Magnus DA, Guerout AM, Mazel D (1999) Super-in-tegrons. Res Microbiol 150:641-651
74. Rowe-Magnus DA, Guerout AM, Ploncard P, Dychinco B, Davies J, Mazel D (2001) The evolutionary history of chromosomal super-integrons provides an ancestry for multiresistant integrons. Proc Natl Acad Sci USA 98:652-657 (Pubitemid 32105098)
75. Sallen B, Rajoharison A, Desvarenne S, Mabilat C (1995) Molecular epidemiology of integron-associated antibiotic resistance genes in clinical isolates of Enterobacteriaceae. Microb Drug Resist 1:91-98
76. Salmenlinna S, Lyytikainen O, Vuopio-Varkila J (2002) Community acquired methicillin resistant Staphylococcus aureus, Finland. Emerg Infect Dis 8:602-607
77. Schito GC (2006) The importance of the development of antibiotic resistance in Staphylococcus aureus. Clin Microbiol Infect 12(Suppl 1):3-8
78. Segal H, Francia MV, Lobo JM, Elisha G (1999) Reconstruction of an active integron recombination site after integration of a gene cassette at a secondary site. Antimicrob Agents Chemother 43:2538-2541 (Pubitemid 29471243)
79. Senda K, Arakawa Y, Ichiyama S (1996) PCR detection of metallo-beta-lactamase gene (blaIMP) in gram-negative rods resistant to broad-spectrum beta-lactams. J Clin Microbiol 34:2909-2913 (Pubitemid 26389332)
80. Seward RJ, Towner KJ (1999) Detection of integrons in worldwide nosocomial isolates of Acinetobacter spp. Clin Microbiol Infect 5:308-318 (Pubitemid 29312608)
81. Shi L, Zheng M, Xiao Z, Asakura M, Su J, Lin L (2006) Unnoticed spread of class 1 integrons in Gram-positive strains isolated in Guangzhou, China. Microbiol Immunol 50:463-467 (Pubitemid 43927235)
82. Shopsin B, Gomez M, Montgomery SO, Smith DH, Waddington M, Dodge DE, Bost DA, Riehman M, Naidich S, Kreiswirth BN (1999) Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol 37:3556-3563 (Pubitemid 29491091)
83. Shopsin B, Gomez M, Waddington M, Riehman M, Kreiswirth BN (2000) Use of coagulase gene (coa) repeat region nucleotide sequences for typing of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 38:3453-3456
84. Stokes HW, Hall RM (1989) A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. Mol Microbiol 3:1669-1683 (Pubitemid 20023246)
85. Stokes HW, O'Gorman DB, Recchia GD, Parsekhian M, Hall RM (1997) Structure and function of 59-base element recombination sites associated with mobile gene cassettes. Mol Microbiol 26:731-745 (Pubitemid 27489335)
86. Su J, Shi L, Yang L, Xiao Z, Li X, Li L, Yamasaki S (2006) Analysis of integrons in clinical isolates of Escherichia coli in China during the last six years. FEMS Microbiol Lett 254:75-80
87. Sundstrom L (1998) The potential of integrons and connected programmed rearrangements for mediating horizontal gene transfer. APMIS Suppl 84:37-42 (Pubitemid 28531438)
88. Sundstrom L, Roy PH, Skold O (1991) Site-specific insertion of three structural gene cassettes in transposon Tn7. J Bacteriol 173:3025-3028
89. Tauch A, Gotker S, Puhler A, Kalinowski J, Thierbach G (2002) The 27.8-kb R-plasmid pTET3 from Corynebacterium glutam-icum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid 48:117-129 (Pubitemid 35300803)
90. Van Belkum A, Kluytmans J, Van Leeuwen W, Bax R, Quint W, Peters E, Fluit A, Vandenbroucke GC, Vanden BA, Koel-eman H, Melchers W, Meis J, Elaichouni A, Vaneechoutte M, Moonens F, Maes N, Struelens M, Tenover F, Verbrugh H (1995) Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains. J Clin Microbiol 33:1537-1547
91. Van Belkum A, Goessens W, Van Der Schee C (2001) Rapid emergence of ciprofloxacin-resistant Enterobacteriaceae containing multiple gentamicin resistance-associated integrons in a Dutch hospital. Emerg Infect Dis 7:862-871 (Pubitemid 33121594)
92. Van Essen-Zandbergen A, Smith H, Veldman K, Mevius D (2007) Occurrence and characteristics of class 1, 2 and 3 in-tegrons in Escherichia coli, Salmonella and Campylobacter spp. in the Netherlands. J Antimicrob Chemother 59:746-750 (Pubitemid 47073411)
93. Vandenesch F, Naimi T, Enright MC, Lina G, Nimmo GR, Heffernan H (2003) Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis 9:978-984 (Pubitemid 36944519)
94. Xu Z, Shi L, Zhang C, Zhang L, Li X, Cao Y, Li L, Yamasaki S (2007) Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China. Clin Microbiol Infect 13:980-984 (Pubitemid 47382253)
95. Xu Z, Shi L, Alam MJ, Li L, Yamasaki S (2008) Integron-bearing methicillin-resistant coagulase-negative staphylococci in South China, 2001-2004. FEMS Microbiol Lett 278:223-230 (Pubitemid 350264580)
96. Xu Z, Li L, Alam MJ, Yamasaki S, Shi L (2008) First confirmation of integron-bearing methicillin-resistant Staphylococcus aureus. Curr Microbiol 57:264-268
97. Xu Z, Li L, Shirtliff ME, Alam MJ, Yamasaki S, Shi L (2009) Occurrence and characteristics of class 1 and 2 integrons in Pseudomonas aeruginosa isolates from patients in Southern China. J Clin Microbiol 47:230-234
98. Xu Z, Li L, Shirtliff ME, Peters BM, Peng Y, Alam MJ, Yamasaki S, Shi L (2010) First report of class 2 integron in clinical Enterococcus faecalis and class 1 integron in Entero-coccus faecium in South China. Diag Microbiol Infect Dis 68:315-317
99. Xu Z, Li L, Shirtliff ME, Peters BM, Li B, Peng Y, Alam MJ, Yamasaki S, Shi L (2010) Resistance class 1 integron in clinical methicillin-resistant Staphylococcus aureus strains in Southern China, 2001-2006. Clin Microbiol Infect. doi:10.1111/j.1469-0691.2010.03379.x
100. Yang H, Chen S, White DG, Zhao S, McDermott P, Walker R, Meng J (2004) Characterization of multiple antimicrobial resistant Escherichia coli isolates from diseased chickens and swine in China. J Clin Microbiol 42:3483-3489 (Pubitemid 39045670)
101. Yu HS, Lee JC, Kang HY, Ro DW, Chung JY, Jeong YS, Tae SH, Choi CH, Lee EY, Soel SY, Lee YC, Cho DT (2003) Changes in gene cassettes of class 1 integrons among Esche-richia coil isolates from urine specimens collected in Korea during the last two decades. J Clin Microbiol 41:5429-5433 (Pubitemid 37543227)
102. Zetola N, Francis JS, Nuermberger EL, Bishai WR (2005) Community-acquired methicillin-resistant Staphylococcus aur-eus: an emerging threat. Lancet Infect Dis 5:275-286 (Pubitemid 40602247)
103. Zhao X, Li Y, Wang L, You L, Xu Z, Li L, He X, Liu Y, Wang J, Yang L (2010) Development and application of a loop-mediated isothermal amplification method on rapid detection Esch-erichia coli O157 strains from food samples. Mol Biol Rep 37:2183-2188