Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop-mediated isothermal amplification assay

Letters in Applied Microbiology

Volumn 45, Issue 1, 2007, Pages 100-107

  Goto, M ^a   Hayashidani, H ^b   Takatori, K ^a   Hara Kudo, Y ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Detection; Enterotoxin; Loop mediated isothermal amplification; Rapid; Staphylococcus aureus

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BACTERIA; DIAGNOSIS; ISOTHERMS; POLYMERASE CHAIN REACTION;

DETECTION; ENTEROTOXIGENIC; ENTEROTOXINS; LOOP MEDIATED ISOTHERMAL AMPLIFICATIONS; LOOP-MEDIATED ISOTHERMAL AMPLIFICATIONS; RAPID; RAPID DETECTION; SPECIFIC PRIMERS; SPECIFIC SEQUENCES; STAPHYLOCOCCUS AUREUS;

GENES;

ENTEROTOXIN; ENTEROTOXIN SEA; ENTEROTOXIN SEB; ENTEROTOXIN SEC; ENTEROTOXIN SED; PRIMER DNA; UNCLASSIFIED DRUG;

BACTERIA; BIOASSAY; FOOD PRODUCTS; GENES;

ASSAY; BACTERIUM; DETECTION METHOD; FOOD SAFETY; GENE; POLYMERASE CHAIN REACTION; TOXIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOASSAY; CONTROLLED STUDY; DIAGNOSTIC VALUE; FOOD SAFETY; GENE IDENTIFICATION; GENE SEQUENCE; GENE TARGETING; INTERMETHOD COMPARISON; LATEX AGGLUTINATION TEST; LOOP MEDIATED ISOTHERMAL AMPLIFICATION ASSAY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

EID: 34347337682     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02142.x     Document Type: Article

References (20)

1. Asao, T., Kumeda, Y., Kawai, T., Shibata, T., Oda, H., Haruki, K., Nakazawa, H. Kozaki, S. (2003) An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiol Infect 130, 33 40.
2. Balaban, N. Rasooly, A. (2000) Staphylococcal enterotoxins. Int J Food Microbiol 61, 1 10.
3. Hara-Kudo, Y., Yoshino, M., Kojima, T. Ikedo, M. (2005) Loop-mediated isothermal amplification for the rapid detection of Salmonella. FEMS Microbiol Lett 253, 155 161.
4. Horisaka, T., Fujita, K., Iwata, T., Nakadai, A., Okatani, A.T., Horikita, T., Taniguchi, T., Honda, E. et al. (2004) Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification. J Clin Microbiol 42, 5349 5352.
5. Johnson, W.M., Tyler, S.D., Ewan, E.P., Ashton, F.E., Pollard, D.R. Rozee, K.R. (1991) Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J Clin Microbiol 29, 426 430.
6. Kitai, S., Shimizu, A., Kawano, J., Sato, E., Nakano, C., Kitagawa, H., Fujino, K., Matsumura, K. et al. (2005) Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan. J Vet Med Sci 67, 269 274.
7. Klotz, M., Opper, S., Heeg, K. Zimmermann, S. (2003) Detection of Staphylococcus aureus enterotoxins A to D by real-time fluorescence PCR assay. J Clin Microbiol 41, 4683 4687.
8. Lina, G., Bohach, G.A., Nair, S.P., Hiramatsu, K., Jouvin-Marche, E. Mariuzza, R. (2004) International nomenclature committee for Staphylococcal superantigens. Standard nomenclature for the superantigens expressed by Staphylococcus. J Infect Dis 189, 2334 2336.
9. Loncarevic, S., Jorgensen, H.J., Lovseth, A., Mathisen, T. Rorvik, L.M. (2005) Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J Appl Microbiol 98, 344 350.
10. Lynn, M.J. Gregory, A.B. (2001) Staphylococcus aureus. In Food Microbiology, 2nd edn ed. Doyle, M.P., Beuchat, L.R. Montville, T.J. pp. 411 434. Washington, DC : American Society for Microbiology.
11. Mori, Y., Nagamine, K., Tomita, N. Notomi, T. (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289, 150 154.
12. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. Hase, T. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28, e63.
13. Ohtsuka, K., Yanagawa, K., Takatori, K. Hara-Kudo, Y. (2005) Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates. Appl Environ Microbiol 71, 6730 6735.
14. Omoe, K., Ishikawa, M., Shimoda, Y., Hu, D.L., Ueda, S. Shinagawa, K. (2002) Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes. J Clin Microbiol 40, 857 862.
15. Omoe, K., Hu, D.L., Takahashi-Omoe, H., Nakane, A. Shinagawa, K. (2003) Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids. Infect Immun 71, 6088 6094.
16. Reginald, W.B. Steven, R.M. (2003) Staphylococcus aureus. In International Hand Book of Foodborne Pathogens. ed. Marianne, D.M. Jeffrey, W.B. pp. 41 60. Basel, NY : Marcel Dekker, Inc.
17. Sambrook, J., Fritsch, E.F. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press.
18. Sharma, N.K., Rees, C.E. Dodd, C.E. (2000) Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains. Appl Environ Microbiol 66, 1347 1353.
19. Song, T., Toma, C., Nakasone, N. Iwanaga, M. (2005) Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method. FEMS Microbiol Lett 243, 259 263.
20. Thomas, D.Y., Jarraud, S., Lemercier, B., Cozon, G., Echasserieau, K., Etienne, J., Gougeon, M.L., Lina, G. et al. (2006) Staphylococcal enterotoxin-like toxins U2 and V, two new staphylococcal superantigens arising from recombination within the enterotoxin gene cluster. Infect Immun 74, 4724 4734.