Expression and purification of natural N-terminal recombinant bovine pancreatic trypsin inhibitor from Pichia pastoris

Biological and Pharmaceutical Bulletin

Volumn 31, Issue 9, 2008, Pages 1680-1685

  Yang, Lili ^a   Dong, Wen ^b   He, Jinchao ^c   Ren, Xiubao ^a   Yan, Weiqun ^c  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b TIANJIN FIRST CENTRAL HOSPITAL   (China)

^c JILIN UNIVERSITY   (China)

Author keywords

Bovine pancreatic trypsin inhibitor; Fermentation; Human serum albumin signal peptide; Pichia pastris; Purification

Indexed keywords

APROTININ; ARGININE; ASPARTIC ACID; CHYMOTRYPSIN; MATING HORMONE ALPHA FACTOR; PLASMA KALLIKREIN; PLASMIN; PROLINE; RECOMBINANT BOVINE PANCREATIC TRYPSIN INHIBITOR; SERINE PROTEINASE INHIBITOR; SERUM ALBUMIN; SIGNAL PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG;

ACUTE HEMORRHAGIC PANCREATITIS; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DRUG DOSE COMPARISON; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAT; SEQUENCE ANALYSIS;

ANIMALS; APROTININ; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; MOLECULAR WEIGHT; PANCREATITIS, ACUTE NECROTIZING; PICHIA; PLASMIDS; RATS; RECOMBINANT PROTEINS; TRYPSIN INHIBITORS;

EID: 51449091144     PISSN: 09186158     EISSN: 13475215     Source Type: Journal    
DOI: 10.1248/bpb.31.1680     Document Type: Article

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