Essential Role of MFG-E8 for Phagocytic Properties of Microglial Cells

PLoS ONE

Volumn 8, Issue 2, 2013, Pages

  Liu, Yong ^a   Yang, Xuesen ^a   Guo, Chenying ^b   Nie, Pan ^c   Liu, Yan ^c   Ma, Jie ^b  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b SCHEPENS EYE RESEARCH INSTITUTE   (United States)

^c SOUTHWEST UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; INTERLEUKIN 1BETA; LACTADHERIN; MESSENGER RNA; PHOSPHATIDYLSERINE; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; BETA ACTIN GENE; CELL PHAGOCYTOSIS; COCULTURE; CONTROLLED STUDY; GENE; HUMAN; HUMAN CELL; IL 1BETA GENE; MICROGLIA; MILK FAT GLOBULE E8 GENE; MOUSE; NEWBORN; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TNF ALPHA GENE;

ANIMALS; ANIMALS, NEWBORN; ANTIGENS, SURFACE; APOPTOSIS; BLOTTING, WESTERN; CELLS, CULTURED; COCULTURE TECHNIQUES; ERYTHROCYTES; HUMANS; INFLAMMATION; MICE; MICE, INBRED C57BL; MICROGLIA; MILK PROTEINS; PHAGOCYTOSIS; PHOSPHATIDYLSERINES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84873530432     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0055754     Document Type: Article

References (38)

1. Mochizuki H, Goto K, Mori H, Mizuno Y, (1996) Histochemical detection of apoptosis in Parkinson's disease. Journal of the Neurological Sciences 137: 120-123.
2. Thomas LB, Gates DJ, Richfield EK, O'Brien TF, Schweitzer JB, et al. (1995) DNA end labeling (TUNEL) in Huntington's disease and other neuropathological conditions. Experimental Neurology 133: 265-272.
3. Zhang L, Kokkonen G, Roth GS, (1995) Identification of neuronal programmed cell death in situ in the striatum of normal adult rat brain and its relationship to neuronal death during aging. Brain Research 677: 177-179.
4. Savill J, Fadok V, (2000) Corpse clearance defines the meaning of cell death. Nature 407: 784-788.
5. Saijo K, Glass CK, (2011) Microglial cell origin and phenotypes in health and disease. Nature Review Immunology 11: 775-787.
6. Rock RB, Gekker G, Hu S, Sheng WS, Cheeran M, et al. (2004) Role of microglia in central nervous system infections. Clinical Microbiology Reviews 17: 942-964.
7. Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RAB, et al. (2000) A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature 405: 85-90.
8. Hanayama R, Tanaka M, Miwa K, Shinohara A, Iwamatsu A, et al. (2002) Identification of a factor that links apoptotic cells to phagocytes. Nature 417: 182-187.
9. Hall MO, Obin MS, Heeb MJ, Burgess BL, Abrams TA, (2005) Both protein S and Gas6 stimulate outer segment phagocytosis by cultured rat retinal pigment epithelial cells. Experimental Eye Research 81: 581-591.
10. Leonardi-Essmann F, Emig M, Kitamura Y, Spanagel R, Gebicke-Haerter PJ, (2005) Fractalkine-upregulated milk-fat globule EGF factor-8 protein in cultured rat microglia. Journal of Neuroimmunology 160: 92-101.
11. Fuller A, Van Eldik L, (2008) MFG-E8 regulates microglial phagocytosis of apoptotic neurons. Journal of Neuroimmune Pharmacology 3: 246-256.
12. Fadok VA, Bratton DL, Konowal A, Freed PW, Westcott JY, et al. (1998) Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-β, PGE2, and PAF. The Journal of Clinical Investigation 101: 890-898.
13. Fricker M, Neher JJ, Zhao JW, Théry C, Tolkovsky AM, et al. (2012) MFG-E8 mediates primary phagocytosis of viable neurons during neuroinflammation. The Journal of Neuroscience 32: 2657-2666.
14. Neher JJ, Neniskyte U, Zhao JW, Bal-Price A, Tolkovsky AM, et al. (2011) Inhibition of microglial phagocytosis is sufficient to prevent inflammatory neuronal death. The Journal of Immunology 186: 4973-4983.
15. Fricker M, Oliva-Martín MJ, Brown GC, (2012) Primary phagocytosis of viable neurons by microglia activated with LPS or Aβ is dependent on calreticulin/LRP phagocytic signalling. Journal of Neuroinflammation 9: 196.
16. Neher JJ, Neniskyte U, Brown GC, (2012) Primary phagocytosis of neurons by inflamed microglia: potential roles in neurodegeneration. Frontiers in Pharmacology 3: 27.
17. Kagan VE, Gleiss B, Tyurina YY, Tyurin VA, Elenström-Magnusson C, et al. (2002) A role for oxidative stress in apoptosis: oxidation and externalization of phosphatidylserine is required for macrophage clearance of cells undergoing Fas-mediated apoptosis. The Journal of Immunology 169: 487-499.
18. Tyurina YY, Serinkan FB, Tyurin VA, Kini V, Yalowich JC, et al. (2004) Lipid antioxidant, etoposide, inhibits phosphatidylserine externalization and macrophage clearance of apoptotic cells by preventing phosphatidylserine oxidation. The Journal of Biological Chemistry 279: 6056-6064.
19. Giulian D, Baker T, (1986) Characterization of ameboid microglia isolated from developing mammalian brain. The Journal of Neuroscience 6: 2163-2178.
20. Roy A, Fung YK, Liu X, Pahan K, (2006) Up-regulation of microglial CD11b expression by nitric oxide. Journal of Biological Chemistry 281: 14971-14980.
21. Oshima K, Aoki N, Kato T, Kitajima K, Matsuda T, (2002) Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles. European Journal of Biochemistry 269: 1209-1218.
22. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, et al. (1998) A third-generation lentivirus vector with a conditional packaging system. Journal of Virology 72: 8463-8471.
23. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, et al. (2009) Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS ONE 4: e5046.
24. Škalamera D, Ranall MV, Wilson BM, Leo P, Purdon AS, et al. (2011) A high-throughput platform for lentiviral overexpression screening of the human ORFeome. PLoS ONE 6: e20057.
25. Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, et al. (1998) Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. Journal of Virology 72: 9873-9880.
26. Geraerts M, Willems S, Baekelandt V, Debyser Z, Gijsbers R, (2006) Comparison of lentiviral vector titration methods. BMC Biotechnology 6: 34.
27. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402-408.
28. Schmittgen TD, Livak KJ, (2008) Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3: 1101-1108.
29. Ravichandran K, (2011) Beginnings of a good apoptotic meal: the find-me and eat-me signaling pathways. Immunity 35: 445-455.
30. Marguet D, Luciani M-F, Moynault A, Williamson P, Chimini G, (1999) Engulfment of apoptotic cells involves the redistribution of membrane phosphatidylserine on phagocyte and prey. Nature Cell Biology 1: 454-456.
31. Hanayama R, Tanaka M, Miyasaka K, Aozasa K, Koike M, et al. (2004) Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice. Science 304: 1147-1150.
32. Neniskyte U, Neher JJ, Brown GC, (2011) Neuronal death induced by nanomolar amyloid beta is mediated by primary phagocytosis of neurons by microglia. The Journal of Biological Chemistry 286: 39904-39913.
33. Aziz M, Jacob A, Matsuda A, Wu R, Zhou M, et al. (2011) Pre-treatment of recombinant mouse MFG-E8 downregulates LPS-induced TNF-α production in macrophages via STAT3-mediated SOCS3 activation. PLoS ONE 6: e27685.
34. Miksa M, Wu R, Dong W, Das P, Yang D, et al. (2006) Dendritic cell-derived exosomes containing milk fat globule epidermal growth factor-factor Viii attenuate proinflammatory responses in sepsis. Shock 25: 586-593.
35. Zeng HY, Zhu X, Zhang C, Yang LP, Wu LM, et al. (2005) Identification of sequential events and factors associated with microglial activation, migration, and cytotoxicity in retinal degeneration in rd mice. Investigative Ophthalmology & Visual Science 46: 2992-2999.
36. Aziz M, Jacob A, Matsuda A, Wang P, (2011) Review: milk fat globule-EGF factor 8 expression, function and plausible signal transduction in resolving inflammation. Apoptosis 16: 1077-1086.
37. Yamaguchi H, Fujimoto T, Nakamura S, Ohmura K, Mimori T, et al. (2010) Aberrant splicing of the milk fat globule-EGF factor 8 (MFG-E8) gene in human systemic lupus erythematosus. European Journal of Immunology 40: 1778-1785.
38. Yamaguchi H, Takagi J, Miyamae T, Yokota S, Fujimoto T, et al. (2008) Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus. Journal of Leukocyte Biology 83: 1300-1307.