A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial cells in minute sample volume followed by direct on-chip quantitative PCR assay

Analytical Chemistry

Volumn 85, Issue 3, 2013, Pages 1484-1491

  Zhang, Rui ^a,b   Gong, Hai Qing ^a   Zeng, Xudong ^c   Lou, Chaoping ^a   Sze, Chunchau ^b,d  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^c Star Array Pte Ltd   (Singapore)

^d NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ACID EXTRACTION; ACID PURIFICATION; ACID RECOVERY; AQUEOUS PHASE; BACTERIAL CELLS; BACTERIAL PATHOGENS; BIOLOGICAL APPLICATIONS; CONTINUOUS FLOWS; DEVICE SET; EFFECTIVE SOLUTION; HEADSPACES; HIGH-THROUGHPUT; INTERFACIAL CONTACT; LIQUID PHASE; MICROFLUIDIC PLATFORMS; MICROWELLS; ON CHIPS; ORGANIC PHASE; PCR ANALYSIS; PHASE PARTITIONING; QUANTITATIVE PCR; SAMPLE VOLUME; SINGLE CELLS; SOLID-PHASE; TRANSCRIPTIONAL PROFILING; TWO DIMENSIONAL (2D) ARRAYS;

BIOMOLECULES; LIQUIDS; MICROFLUIDICS; PHENOLS; PURIFICATION; RNA;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; BACTERIAL RNA; BOVINE SERUM ALBUMIN; NUCLEIC ACID;

ANIMAL; ARTICLE; CATTLE; DNA MICROARRAY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROFLUIDICS; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; CATTLE; DNA, BACTERIAL; MICROFLUIDICS; NUCLEIC ACIDS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, BACTERIAL; SERUM ALBUMIN, BOVINE;

EID: 84873338421     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac3026509     Document Type: Article

References (29)

1. Roman, G. T.; Kennedy, R. T. J. Chromatogr., A 2007, 1168, 170-188
2. Mahalanabis, M.; Al-Muayad, H.; Kulinski, M. D.; Altman, D.; Klapperich, C. M. Lab Chip 2009, 9, 2811-2817
3. Liu, R. H.; Yang, J.; Lenigk, R.; Bonanno, J.; Grodzinski, P. Anal. Chem. 2004, 76, 1824-1831
4. Belgrader, P.; Hansford, D.; Kovacs, G. T. A.; Venkateswaran, K.; Mariella, R., Jr.; Milanovich, F.; Nasarabadi, S.; Okuzumi, M.; Pourahmadi, F.; Northrup, M. A. Anal. Chem. 1999, 71, 4232-4236
5. Hindson, B. J.; Gutierrez, D. M.; Ness, K. D.; Makarewicz, A. J.; Metz, T. R.; Setlur, U. S.; Benett, W. B.; Loge, J. M.; Colston, J. B. W.; Francis, P. S.; Barnett, N. W.; Dzenitis, J. M. Analyst 2008, 133, 248-255
6. Cady, N. C.; Stelick, S.; Batt, C. A. Biosens. Bioelectron. 2003, 19, 59-66
7. Boom, R.; Sol, C. J.; Salimans, M. M.; Jansen, C. L.; Wertheim-van Dillen, P. M.; van der Noordaa, J. J. Clin. Microbiol. 1990, 28, 495-503
8. Akutsu, J.-i.; Tojo, Y.; Segawa, O.; Obata, K.; Okochi, M.; Tajima, H.; Yohda, M. Biotechnol. Bioeng. 2004, 86, 667-671
9. Liu, C.-J.; Lien, K.-Y.; Weng, C.-Y.; Shin, J.-W.; Chang, T.-Y.; Lee, G.-B. Biomed. Microdevices 2009, 11, 339-350
10. Hong, J. W.; Studer, V.; Hang, G.; Anderson, W. F.; Quake, S. R. Nat. Biotechol. 2004, 22, 435-439
11. Muyal, J.; Muyal, V.; Kaistha, B.; Seifart, C.; Fehrenbach, H. Diagn. Pathol. 2009, 4, 9
12. Kramvis, A.; Bukofzer, S.; Kew, M. C. J. Clin. Microbiol. 1996, 34, 2731-2733
13. Xiang, X.; Qiu, D.; Hegele, R. D.; Tan, W. C. J. Virol. Methods 2001, 94, 129-135
14. Morales, M.; Zahn, J. Microfluid. Nanofluid. 2010, 9, 1041-1049
15. Liu, H.-B; Gong, H.-Q. J. Micromech. Microeng. 2009, 19, 037002
16. Liu, H.-B.; Ramalingam, N.; Jiang, Y.; Dai, C.-C.; Hui, K. M.; Gong, H.-Q. Sens. Actuators, B 2009, 135, 671-677
17. Mazutis, L.; Araghi, A. F.; Miller, O. J.; Baret, J.-C.; Frenz, L.; Janoshazi, A.; Taly, V.; Miller, B. J.; Hutchison, J. B.; Link, D.; Griffiths, A. D.; Ryckelynck, M. Anal. Chem. 2009, 81, 4813-4821
18. Bhagwat, A. A.; Phadke, R. P.; Wheeler, D.; Kalantre, S.; Gudipati, M.; Bhagwat, M. J. Microbiol. Methods 2003, 55, 399-409
19. Nanassy, O. Z.; Haydock, P. V.; Reed, M. W. Anal. Biochem. 2007, 365, 240-245
20. Khan, I. U. H.; Yadav, J. S. J. Clin. Microbiol. 2004, 42, 453-457
21. De Medici, D.; Croci, L.; Delibato, E.; Di Pasquale, S.; Filetici, E.; Toti, L. Appl. Environ. Microbiol. 2003, 69, 3456-3461
22. Sung, K.; Khan, S. A.; Nawaz, M. S.; Khan, A. A. FEMS Microbiol. Lett. 2003, 229, 97-101
23. Kubista, M.; Andrade, J. M.; Bengtsson, M.; Forootan, A.; Jonák, J.; Lind, K.; Sindelka, R.; Sjöback, R.; Sjögreen, B.; Strömbom, L.; StaÌŠhlberg, A.; Zoric, N. Mol. Aspects Med. 2006, 27, 95-125
24. Salonen, A.; Nikkilä, J.; Jalanka-Tuovinen, J.; Immonen, O.; Rajilić-Stojanović, M.; Kekkonen, R. A.; Palva, A.; de Vos, W. M. J. Microbiol. Methods 2010, 81, 127-134
25. Amagliani, G.; Giammarini, C.; Omiccioli, E.; Brandi, G.; Magnani, M. Food Control 2007, 18, 1137-1142
26. Phongsisay, V.; Perera, V. N.; Fry, B. N. J. Microbiol. Methods 2007, 68, 427-429
27. Gao, W.; Zhang, W.; Meldrum, D. R. J. Microbiol. Methods 2011, 85, 221-227
28. Peérez-Osorio, A. C.; Williamson, K. S.; Franklin, M. J. J. Bacteriol. 2010, 192, 2991-3000
29. Zhang, L.; Fan, F.; Palmer, L. M.; Lonetto, M. A.; Petit, C.; Voelker, L. L.; St. John, A.; Bankosky, B.; Rosenberg, M.; McDevitt, D. Gene 2000, 255, 297-305