Droplet enhanced microfluidic-based DNA purification from bacterial lysates via phenol extraction

Microfluidics and Nanofluidics

Volumn 9, Issue 6, 2010, Pages 1041-1049

  Morales, Mercedes C ^a   Zahn, Jeffrey D ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

DNA purification; Droplet based flow; Phenol extraction

Indexed keywords

AQUEOUS INTERFACES; AQUEOUS PHASE; CELL LYSATES; CONVECTIVE FLOW; DNA MANIPULATION; DNA PURIFICATION; DNA SAMPLE PREPARATION; DROPLET-BASED FLOW; FLOW CONDITION; GENOMIC ANALYSIS; LIQUID EXTRACTION; LYSATES; MACRO SCALE; MATERIAL TRANSPORT; MICRO DEVICES; MICRO-SCALES; MICROFLUIDIC PLATFORMS; ON CHIPS; ORGANIC PHASE; PHENOL EXTRACTION; PLASMID DNA; PLASMID RECOVERY; PROTEIN PARTITIONING; TWO PHASE SYSTEMS;

BACTERIOLOGY; DNA; DROP FORMATION; MICROFLUIDICS; PHASE INTERFACES; PHENOLS; PROTEINS; PURIFICATION; SERPENTINE; SILICATE MINERALS; SOLVENT EXTRACTION;

CHEMICALS REMOVAL (WATER TREATMENT);

EID: 78649734354     PISSN: 16134982     EISSN: 16134990     Source Type: Journal    
DOI: 10.1007/s10404-010-0623-2     Document Type: Article

References (33)

1. J Atencia DJ Beebe 2005 Controlled microfluidic interfaces Nature 437 648 655 10.1038/nature04163 (Pubitemid 41486527)
2. A Bhattacharyya CM Klapperich 2008 Microfluidics-based extraction of viral RNA from infected mammalian cells for disposable molecular diagnostics Sens Actuators B 129 693 698 10.1016/j.snb.2007.09.057 (Pubitemid 351221283)
3. MC Breadmore KA Wolfe IG Arcibal WK Leung D Dickson BC Giordano ME Power JP Ferrance SH Feldman PM Norris JP Landers 2003 Microchip-based purification of DNA from biological samples Anal Chem 75 1880 1886 10.1021/ac0204855 (Pubitemid 36438011)
4. X Chen F da Cui CC Liu 2008 On-line cell lysis and DNA extraction on a microfluidic biochip fabricated by microelectromechanical system technology Electrophoresis 29 1844 1851 10.1002/elps.200700551 (Pubitemid 351663880)
5. LA Christel K Petersen W McMillan MA Northrup 1999 Rapid, automated nucleic acid probe assays using silicon microstructures for nucleic acid concentration J Biomech Eng 121 22 27 10.1115/1.2798037
6. GF Christopher SL Anna 2007 Microfluidic methods for generating continuous droplet streams J Phys D 40 R319 R336 10.1088/0022-3727/40/19/R01 (Pubitemid 47469862)
7. R Dreyfus P Tabeling H Willaime 2003 Ordered and disordered patterns in two-phase flows in microchannels Phys Rev Lett 90 144505 10.1103/PhysRevLett.90. 144505
8. CJ Easley JM Karlinsey JM Bienvenue LA Legendre MG Roper SH Feldman MA Hughes EL Hewlett TJ Merkel JP Ferrance JP Landers 2006 A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability Proc Natl Acad Sci USA 103 19272 19277 10.1073/pnas.0604663103 (Pubitemid 46026023)
9. P Guillot A Colin 2005 Stability of parallel flows in a microchannel after a T junction Phys Rev E 72 066301 066304 10.1103/PhysRevE.72.066301 (Pubitemid 41777650)
10. A Gunther KF Jensen 2007 Multiphase microfluidics: from flow characteristics to chemical and materials synthesis Lab Chip 7 935 938 10.1039/b708898c (Pubitemid 47000825)
11. KA Hagan WL Meier JP Ferrance JP Landers 2009 Chitosan-coated silica as a solid phase for RNA purification in a microfluidic device Anal Chem 81 5249 5256 10.1021/ac900820z
12. K Haubert T Drier D Beebe 2006 PDMS bonding by means of a portable, low-cost corona system Lab Chip 6 1548 1549 10.1039/b610567j (Pubitemid 46100751)
13. Hibara A, Kasai K, Miyaguchi H, Kitamori T (2008) Novel two-phase flow control concept and multistep extraction microchip. In: Proceedings of the 12th international conference on miniaturized systems for chemistry and life sciences (mTAS 2008), San Diego, CA, pp 1326-1328
14. MD Kulinski M Mahalanabis S Gillers JY Zhang S Singh CM Klapperich 2009 Sample preparation module for bacterial lysis and isolation of DNA from human urine Biomed Microdevices 11 671 678 10.1007/s10544-008-9277-1
15. ET Lagally CA Emrich RA Mathies 2001 Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis Lab Chip 1 102 107 10.1039/b109031n
16. LA Legendre JM Bienvenue MG Roper JP Ferrance JP Landers 2006 A simple, valveless microfluidic sample preparation device for extraction and amplification of DNA from nanoliter-volume samples Anal Chem 78 1444 1451 10.1021/ac0516988 (Pubitemid 43346278)
17. Mao X, Yang S, Zahn J (2006) Experimental demonstration and numerical simulation of organic-aqueous liquid extraction enhanced by droplet formation in a microfluidic channel. In: Proceedings of the ASME-IMECE, IMECE 2006-16084, Chicago, IL
18. Morales M, Zahn J (2008) Development of a diffusion limited microfluidic module for dna purification via phenol extraction. In: Proceedings of the ASME-IMECE, IMECE 2008-68086, Boston, MA
19. Qiagen (2003) QIAprep miniprep handbook. Qiagen Distributors
20. V Reddy JD Zahn 2005 Interfacial stabilization of organic-aqueous two-phase microflows for a miniaturized DNA extraction module J Colloid Interface Sci 286 158 165 10.1016/j.jcis.2004.12.052 (Pubitemid 40569358)
21. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, NY
22. L Shui JCT Eijkel A van den Berg 2007 Multiphase flow in micro- and nanochannels Sens Actuators B 121 263 276 10.1016/j.snb.2006.09.040 (Pubitemid 46123903)
23. L Shui JCT Eijkel A van den Berg 2007 Multiphase flow in microfluidic systems-control and applications of droplets and interfaces Adv Colloid Interface Sci 133 35 49 10.1016/j.cis.2007.03.001 (Pubitemid 47304430)
24. Sober H, Harte R (1968) Handbook of biochemistry selected data for molecular biology. The Chemical Rubber Company, Cleveland, OH
25. H Song D Chen R Ismagilov 2006 Reactions in droplets in microfluidic channels Angew Chem Int Ed 45 7336 7356 10.1002/anie.200601554 (Pubitemid 44786956)
26. H Tian AFR Hühmer JP Landers 2000 Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format Anal Biochem 283 175 191 10.1006/abio.2000.4577 (Pubitemid 30626269)
27. JD Tice H Song AD Lyon RF Ismagilov 2003 Formation of droplets and mixing in multiphase microfluidics at low values of the Reynolds and the capillary numbers Langmuir 19 9127 9133 10.1021/la030090w
28. J Tice A Lyon R Ismagilov 2004 Effects of viscosity on droplet formation and mixing in microfluidic channels Anal Chim Acta 507 73 77 10.1016/j.aca.2003.11.024 (Pubitemid 38224091)
29. J Wen C Guillo JP Ferrance JP Landers 2007 Microfluidic-based DNA purification in a two-stage, dual-phase microchip containing a reversed-phase and a photopolymerized monolith Anal Chem 79 6135 6142 10.1021/ac0703698 (Pubitemid 47282323)
30. QR Wu JM Bienvenue BJ Hassan YC Kwok BC Giordano PM Norris JP Landers JP Ferrance 2006 Microchip-based macroporous silica sol-gel monolith for efficient isolation of DNA from clinical samples Anal Chem 78 5704 5710 10.1021/ac060390t (Pubitemid 44265030)
31. Y Xia GM Whitesides 1998 Soft lithography Angew Chem Int Ed 37 550 575 10.1002/(SICI)1521-3773(19980316)37:5<550::AID-ANIE550>3.0.CO;2-G
32. J Xu S Li J Tan G Luo 2008 Correlations of droplet formation in T-junction microfluidic devices: from squeezing to dripping Microfluid Nanofluid 5 711 717 10.1007/s10404-008-0306-4
33. J Zahn V Reddy 2006 Two phase micromixing and analysis using electrohydrodynamic instabilities Microfluid Nanofluid 2 399 415 10.1007/s10404-006-0082-y (Pubitemid 44303935)