Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action

Gene

Volumn 255, Issue 2, 2000, Pages 297-305

  Zhang, Ling ^a   Fan, Frank ^a   Palmer, Leslie M ^a   Lonetto, Michael A ^a   Petit, Chantal ^a   Voelker, Leroy L ^a   St John, Annemarie ^a   Bankosky, Brent ^a   Rosenberg, Martin ^a   McDevitt, Damien ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Methionyl tRNA synthetase; Polypeptide deformylase; Regulated promoter; Spac; Xyl; Xyl tet

Indexed keywords

ENZYME INHIBITOR; POLYPEPTIDE DEFORMYLASE; PSEUDOMONIC ACID; SB 220334; UNCLASSIFIED DRUG;

ANTIBACTERIAL ACTIVITY; ANTIBIOTIC SENSITIVITY; ARTICLE; CELL VIABILITY; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; IMMUNOBLOTTING; INTERMETHOD COMPARISON; PHENOTYPE; PRIORITY JOURNAL; REPRESSOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; TECHNIQUE;

ANTIBIOTICS; BETA-GALACTOSIDASE; CELL DIVISION; DRUG RESISTANCE, MICROBIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, ESSENTIAL; GENES, LETHAL; LAC OPERON; MEMBRANE PROTEINS; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; PLASMIDS; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS; SUPPORT, U.S. GOV'T, NON-P.H.S.; TETRACYCLINE; XYLOSE;

BACILLUS SUBTILIS; ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

EID: 0034687158     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00325-5     Document Type: Article

References (28)

1. Apfel C.M., Takacs S., Fountoulakis M., Stieger M., Keck W. Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: cloning, expression, and characterization of the essential uppS gene. J. Bacteriol. 181:1999;483-492.
2. Bruckner R. A series of shuttle vectors for Bacillus subtilis and Escherichia coli. Gene. 122:1992;187-192.
3. Chan P.F., Foster S.J. The role of environmental factors in the regulation of virulence-determinant expression in Staphylococcus aureus 8325-4. Microbiology. 144:1998;2469-2479.
4. Chang S.Y.P., McGary E.C., Chang S. Methionine aminopeptidase gene of Escherichia coli is essential for cell growth. J. Bacteriol. 171:1989;4070-4072.
5. Chen D.Z., Petel D.V., Hackbarth C.J., Wang W., Dreyer G., Young D.C., Margolis P.S., Wu C., Ni Z.J.T.J., White R.J., Yuan Z. Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor. Biochemistry. 39:2000;1256-1262.
6. Eichenbaum Z., Federle M.J., Marra D., Devos W.M., Kuipers O.P., Kleerebezem M., Scott J.R. Use of the lactococcal nisa promoter to regulate gene expression in gram-positive bacteria - comparison of induction level and promoter strength. Appl. Environ. Microbiol. 64:1998;2763-2769.
7. Geissendorfer M., Hillen W. Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements. Appl. Microbiol. Biotechnol. 33:1990;657-663.
8. Gregory P.D., Lewis R.A., Curnock S.P., Dyke K.G. Studies of the repressor (BlaI) of beta-lactamase synthesis in Staphylococcus aureus. Mol. Microbiol. 24:1997;1025-1037.
9. BAD promoter. J. Bacteriol. 177:1995;4121-4130.
10. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:1996;986-994.
11. Holland P.M., Abramson R.D., Watson R., Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA. 88:1991;7276-7280.
12. Iordanescu S. Characterization of the Staphylococcus aureus chromosomal gene pcrA, identified by mutations affecting plasmid pT181 replication. Mol. Gen. Genet. 241:1993;185-192.
13. Ji G., Silver S. Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid pI258. J. Bacteriol. 174:1992;3684-3694.
14. Ji Y., Marra A., Rosenberg M., Woodnutt G. Regulated antisense RNA eliminates α-toxin virulence in Staphylococcus aureus infection. J. Bacteriol. 181:1999;6585-6590.
15. Kim L., Mogk A., Schumann W. A xylose-inducible Bacillus subtilis integration vector and its application. Gene. 181:1996;71-76.
16. Kraus A., Iandolo J.J. High-frequency transformation of Staphylococcus aureus by electroporation. Curr. Microbiol. 21:1990;373-376.
17. Kreiswirth B.N., Lofdahl S., Betley M.J., O'Reilly M., Schlievert P.M., Bergdoll M.S., Novick R.P. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature. 305:1983;709-712.
18. Meinnel T., Blanquet S. Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNAfmet formyltransferase. J. Bacteriol. 176:1994;7387-7390.
19. Approved Standard M7-A4. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 1997;National Committee for Clinical Laboratory Standards, Villanova, PA.
20. Nicholson W.L., Setlow P. Sporulation, germination and outgrowth. Molecular Biological Methods for Bacillus. 1990;391-429 Wiley, Chichester, UK.
21. Novick R.P. Genetic systems in Staphylococci. Methods Enzymol. 204:1991;587-636.
22. Oskouian B., Stewart G.C. Cloning and characterization of the repressor gene of the Staphylococcus aureus lactose operon. J. Bacteriol. 169:1987;5459-5465.
23. Petit M.A., Dervyn E., Rose M., Entian K.D., McGovern S., Ehrlich S.D., Bruand C. PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication. Mol. Microbiol. 29:1998;261-273.
24. Rohrer S., Ehlert K., Tschierske M., Labischinski H., Berger-Bachi B. The essential Staphylococcus aureus gene fmhB is involved in the first step of peptidoglycan pentaglycine interpeptide formation. Proc. Natl. Acad. Sci. USA. 96:1999;9351-9356.
25. Schneewind O., Model P., Fischetti V.A. Sorting of protein A to the staphylococcal cell wall. Cell. 70:1992;267-281.
26. Stieger M., Wohlgensinger B., Kamber M., Rolf L., Keck W. Integrational plasmids for the tetracycline-regulated expression of genes in Streptococcus pneumoniae. Gene. 226:1999;243-251.
27. Vagner V., Dervyn E., Ehrlich S.D. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology. 144:1998;3097-3104.
28. Yansura D.G., Henner D.J. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proc. Natl. Acad. Sci. USA. 81:1984;439-443.