Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction

Analytical Biochemistry

Volumn 419, Issue 2, 2011, Pages 161-167

  Von Kanel, Thomas ^a,d   Gerber, Dominik ^a   Wittwer, Carl T ^b,c   Hermann, Mark ^b,c   Gallati, Sabina ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^c ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

^d CANTONAL HOSPITAL AARAU   (Switzerland)

Author keywords

Positional effects; Precision; Real time quantitative PCR; Temperature effects

Indexed keywords

DIAGNOSIS; INSTRUMENT ERRORS; INVERSE PROBLEMS; SYSTEMATIC ERRORS; TEMPERATURE DISTRIBUTION; THERMAL EFFECTS;

ANNEALING TEMPERATURES; DENATURATION TEMPERATURES; POSITIONAL EFFECTS; PRECISION; QUANTITATIVE POLYMERASE CHAIN REACTION; REAL-TIME QUANTITATIVE PCR; TEMPERATURE VARIATION; TEMPERATURE-DEPENDENT EFFECTS;

POLYMERASE CHAIN REACTION;

DNA;

AMPLICON; ARTICLE; CONTROLLED STUDY; DNA DENATURATION; HAPPY PUPPET SYNDROME; HUMAN; PRADER WILLI SYNDROME; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TEMPERATURE DEPENDENCE;

EID: 80054074543     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.08.041     Document Type: Article

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