Te use of melting curves as a novel approach for validation of real-time PCR instruments

BioTechniques

Volumn 51, Issue 3, 2011, Pages 179-184

  von Keyserling, Helmut ^a   Bergmann, Thomas ^a   Wiesel, Moritz ^a   Kaufmann, Andreas M ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

High resolution melting analysis; qPCR; Real time PCR; Thermal cycler validation

Indexed keywords

ARTICLE; HIGH RESOLUTION MELTING ANALYSIS; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; TEMPERATURE; VALIDATION PROCESS; HUMAN; INSTRUMENTATION; QUALITY CONTROL; STANDARD; TRANSITION TEMPERATURE; VALIDATION STUDY;

HUMANS; QUALITY CONTROL; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; TRANSITION TEMPERATURE;

EID: 80053443652     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113735     Document Type: Article

References (11)

1. Holland, P.M., R.D. Abramson, R. Watson, and D.H. Gelfand. 1991. Detection of specific polymerase chain reaction product by utilizing the 5′----3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88:7276-7280.
2. Ginzinger, D.G. 2002. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol. 30:503-512.
3. Stahlberg, A., M. Kubista, and M. Pfaffl. 2004. Comparison of reverse transcriptases in gene expression analysis. Clin. Chem. 50:1678-1680.
4. Bustin, S.A. and T. Nolan. 2004. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15:155-166.
5. Kim, Y.H., I. Yang, Y.S. Bae, and S.R. Park. 2008. Performance evaluation of thermal cyclers for PCR in a rapid cycling condition. BioTechniques 44:495-500.
6. Saunders, G.C., J. Dukes, H.C. Parkes, and J.H. Cornett. 2001. Interlaboratory study on thermal cycler performance in controlled PCR and random amplified polymorphic DNA analyses. Clin. Chem. 47:47-55.
7. Yang, I., Y.H. Kim, J.Y. Byun, and S.R. Park. 2005. Use of multiplex polymerase chain reactions to indicate the accuracy of the annealing temperature of thermal cycling. Anal. Biochem. 338:192-200.
8. Malorny, B., P.T. Tassios, P. Radstrom, N. Cook, M. Wagner, and J. Hoorfar. 2003. Standardization of diagnostic PCR for the detection of foodborne pathogens. Int. J. Food Microbiol. 83:39-48.
9. Schoder, D., A. Schmalwieser, G. Schauberger, J. Hoorfar, M. Kuhn, and M. Wagner. 2005. Novel approach for assessing performance of PCR cyclers used for diagnostic testing. J. Clin. Microbiol. 43:2724-2728.
10. Grønlund, H. A., C. L öfström, J.B. Hel leskov, and J. Hoorfar. 2009. The use of infrared thermography as a novel approach for real-time validation of PCR thermal cyclers. Food Anal. Methods. 3:116-119.
11. Nakano, S., M. Fujimoto, H. Hara, and N. Sugimoto. 1999. Nucleic acid duplex stabilit y: influence of base composition on cation effects. Nucleic Acids Res. 27:2957-2965.