Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii

Fish and Shellfish Immunology

Volumn 33, Issue 5, 2012, Pages 1167-1173

  Li, Meng ^a,b   Zhu, Ling ^a   Zhou, Chun ya ^a,c   Sun, Shan ^a   Fan, Yan jun ^a   Zhuang, Zhi meng ^a  

^a YELLOW SEA FISHERIES RESEARCH INSTITUTE   (China)

^b DALIAN OCEAN UNIVERSITY   (China)

^c SHANGHAI OCEAN UNIVERSITY   (China)

Author keywords

Big defensin; Gram negative microbial challenge; Quantitative real time PCR; Scapharca broughtonii; Tissue distribution

Indexed keywords

ARCIDAE; BRANCHIOSTOMA; BRANCHIOSTOMA LANCEOLATUM; LISTONELLA ANGUILLARUM; MEROSTOMATA; MOLLUSCA; NEGIBACTERIA; SCAPHARCA; SCAPHARCA BROUGHTONII;

EID: 84872604907     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2012.09.008     Document Type: Article

References (49)

1. Fearon D.T., Locksley R.M. The instructive role of innate immunity in the acquired immune response. Science 1996, 272:50-54.
2. Boman H.G. Antibacterial peptides: basic facts and emerging concepts. J Intern Med 2003, 254:197-215.
3. Supungul P., Tang S., Maneeruttanarungroj C., Rimphanitchayakit V., Hirono I., Aoki T., et al. Cloning, expression and antimicrobial activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus monodon. Dev Comp Immunol 2008, 32:61-70.
4. Hultmark D., Steiner H., Rasmuson T., Boman H.G. Insect immunity. Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. Eur J Biochem 1980, 106:7-16.
5. Steiner H., Hultmark D., Engstrom A., Bennich H., Boman H.G. Sequence and specificity of two antimicrobial proteins involved in insect immunity. Nature 1981, 292:246-248.
6. Reddy K.V.R., Yedery R.D., Aranha C. Antimicrobial peptides: premises and promises. Int J Antimicrob Agents 2004, 24:536-547.
7. Cammers-Goodwin A., Allen T.J., Oslick S.L., Mcclure K.F., Lee J.H., Kemp D.S. Mechanism of stabilization of helical confirmations of polypeptides by water containing trifuoroethanol. J Am Chem Soc 1996, 118:3082-3090.
8. Landon C., Sodano P., Hetru C., Hoffmann J., Ptak M. Solution structure of drosomycin, the first inducible antifungal protein from insects. Protein Sci 1997, 6:1878-1884.
9. 7]-polyphemusin ii), determined by nuclear magnetic resonance. BBA 1993, 1163:209-216.
10. Zanetti M., Gennaro R., Romeo D. The cathelicidins family of antimicrobial peptide precursors: a component of the oxygen independent defense mechanisms neutrophils. Ann N Y Acad Sci 1997, 832:147-162.
11. Fregeau Gallagher N.L., Sailer M., Niemczura W.P., Makashima T.T., Stiles M.E., Vederas J.C. Three-dimensional structure of leucocin A in trifluoroethanol and dodecylphosphocholine micelles: spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria. Biochemistry 1997, 36:15062-15072.
12. Brogden K.A. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nat Rev Microbiol 2005, 3:238-250.
13. Charlet M., Chernysh S., Philippe H., Hetru C., Hoffmann J.A., Bulet P. Innate immunity: isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J Biol Chem 1996, 271:21808-21813.
14. Mitta G., Hubert F., Noël T., Roch P. Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. Eur J Biochem 1999, 265:71-78.
15. Mitta G., Vandenbulcke F., Hubert F., Roch P. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci 1999, 112:4233-4242.
16. Mitta G., Hubert F., Dyrynda E.A., Boudry P., Roch P. Mytilin B and MGD2, two antimicrobial peptides of marine mussels: gene structure and expression analysis. Dev Comp Immunol 2000, 24:381-393.
17. Mitta G., Vandenbulcke F., Hubert F., Salzet M., Roch P. Involvement of mytilins in mussel antimicrobial defense. J Biol Chem 2000, 275:12954-12962.
18. Mitta G., Vandenbulcke F., Noel T., Romestand B., Beauvillain J.C., Salzet M., et al. Differential distribution and defence involvement of antimicrobial peptides in mussel. J Cell Sci 2000, 113:2759-2769.
19. Yang Y.S., Mitta G., Chavanieu A., Calas B., Sanchez J.F., Roch P., et al. Solution structure and activity of the synthetic four-disulfide bond Mediterranean mussel defensin (MGD-1). Biochemistry 2000, 39:14436-14447.
20. Pallavicini A., del Mar Costa M., Gestal C., Dreos R., Figueras A., Venier P., et al. High sequence variability of myticin transcripts in hemocytes of immune-stimulated mussels suggests ancient host-pathogen interactions. Dev Comp Immunol 2008, 32:213-226.
21. Roch P., Yang Y., Toubiana M., Aumelas A. NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides. Dev Comp Immunol 2008, 32:227-238.
22. Sonthi M., Toubiana M., Pallavicini A., Venier P., Roch P. Diversity of coding sequences and gene structures of the antifungal peptide mytimycin (MytM) from the mediterranean mussel, Mytilus galloprovincialis. Marine Biotechnol 2011, 13:857-867.
23. Gerdol M., De Moro G., Manfrin C., Venier P., Pallavicini A. Big defensins and mytimacins, new AMP families of the Mediterranean mussel Mytilus galloprovincialis. Dev Comp Immunol 2012, 36:390-399.
24. Gueguen Y., Herpin A., Aumelas A., Garnier J., Fievet J., Escoubas J.M., et al. Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression. J Biol Chem 2006, 28:313-323.
25. Gonzalez M., Gueguen Y., Desserre G., de Lorgeril J., Romestand B., Bachère E. Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas. Dev Comp Immunol 2007, 31:332-339.
26. Schmitt P., Gueguen Y., Desmarais E., Bachère E., de Lorgeril J. Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas. BMC Evol Biol 2010, 25(10):23.
27. Rosa R.D., Santini A., Fievet J., Bulet P., Destoumieux-Garzón D., Bachère E. Big defensins, a diverse family of antimicrobial peptides that follows different patterns of expression in hemocytes of the oyster Crassostrea gigas. PLoS One 2011, 6:e25594.
28. Seo J.K., Crawford J.M., Stone K.L., Noga E.J. Purification of a novel arthropod defensin from the American oyster, Crassostrea virginica. Biochem Biophys Res Commun 2005, 338:1998-2004.
29. Zhao J., Song L., Li C., Ni D., Wu L., Zhu L., et al. Molecular cloning, expression of a big defensin gene from bay scallop Argopecten irradians and the antimicrobial activity of its recombinant protein. Mol Immunol 2007, 44:360-368.
30. Zhao J., Li C., Chen A., Li L., Su X., Li T. Molecular characterization of a novel big defensin from clam Venerupis philippinarum. PLoS ONE 2010, 5:e13480.
31. Adhya M., Jeung H.D., Kang H.S., Choi K.S., Lee D.S., Cho M. Cloning and localization of MCdef, a defensin from Manila clams (Ruditapes philippinarum). Comp Biochem Physiol B 2012, 161:25-31.
32. Xu Q., Wang G., Yuan H., Chai Y., Xiao Z. cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii. Comp Biochem Physiol B 2010, 157:119-126.
33. De Zoysa M., Whang I., Lee Y., Lee S., Lee J.S., Lee J. Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. Fish Shellfish Immunol 2010, 28:261-266.
34. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.
35. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
36. Von H.G. A new method for predicting signal sequence cleavage sites. Nucl Acids Res 1986, 14:4683-4690.
37. Chen Y., Yu P., Luo J., Jiang Y. Secreted protein prediction system combining CJ-SPHMM, TMHMM, and PSORT. Mamm Genome 2003, 14:859-865.
38. Zhu S., Gao B. Evolutionary origin of β-defensins. Dev Comp Immunol 2012, 10.1016/j.dci.2012.02.011.
39. Hancock R.E.W., Sahl H.G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol 2006, 24:1551-1557.
40. Otero-González A.J., Simas Magalhães B., Garcia-Villarino M., López-Abarrategui C., Amaro Sousa D., Campos Dias S., et al. Antimicrobial peptides from marine invertebrates as a new frontier for microbial infection control. FASEB J 2010, 24:1320-1334.
41. Li C., Song L., Zhao J. A review of advances in research on marine molluscan antimicrobial peptides and their potential application in aquaculture. Molluscan Res 2009, 29:17-26.
42. Sperstad S., Haug T., Blencke H.-M., Styrvold O.B., Li C., Stensvåg K. Antimicrobial peptides from marine invertebrates: challenges and perspectives in marine antimicrobial peptide discovery. Biotechnol Adv 2011, 29:519-530.
43. Teng L., Gao B., Zhang S. The first chordate big defensin: identification, expression and bioactivity. Fish Shellfish Immunol 2012, 324:572-577.
44. Johansson M.W., Keyser P., Sritunyalucksana K., Söderhäll K. Crustacean haemocytes and haematopoiesis. Aquaculture 2000, 191:45-52.
45. Iwanaga S., Lee B.L. Recent advances in the innate immunity of invertebrate animals [J]. J Biochem Mol Biol 2005, 38:128-150.
46. Rowley A.F., Powell A. Invertebrate immune systems-specific, quasi-specific, or nonspecific? [J]. J Immunol 2007, 179:7209-7214.
47. Mu C., Zheng P., Zhao J., Wang L., Qiu L., Zhang H., et al. A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol 2011, 31:142-147.
48. Yue F., Pan L.Q., Miao J.J., Zhang L., Li J. Molecular cloning, characterization and mRNA expression of two antibacterial peptides: crustin and anti-lipopolysaccharide factor in swimming crab Portunus trituberculatus. Comp Biochem Physiol B 2010, 156:77-85.
49. Supungul P., Klinbunga S., Pichyangkura R., Hirono I., Aoki T., Tassanaka-jon A. Antimicrobial peptides discovered in the black tiger shrimp Penaeus monodon using the EST approach. Dis Aquat Org 2004, 61:123-135.