Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis

European Journal of Biochemistry

Volumn 265, Issue 1, 1999, Pages 71-78

  Mitta, Guillaume ^a   Hubert, Florence ^a   Noël, Thierry ^a   Roch, Philippe ^a,b  

^a IFREMER   (France)

^b UNIV MONTPELLIER   (France)

Author keywords

Antibacterial peptide; CDNA; Mollusc immunity; Mytilus; Precursor

Indexed keywords

AMINO ACID; CELL PROTEIN; COMPLEMENTARY DNA; CYSTEINE; MYTICIN; PLASMA PROTEIN; UNCLASSIFIED DRUG;

ANTIBACTERIAL ACTIVITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; FUSARIUM OXYSPORUM; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; MUSSEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; ANTIFUNGAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BASE SEQUENCE; BIVALVIA; BLOOD PROTEINS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GRAM-POSITIVE BACTERIA; HEMOCYTES; HEMOLYMPH; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS; RNA, MESSENGER; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; TISSUE DISTRIBUTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FUNGI; FUSARIUM; FUSARIUM OXYSPORUM; MOLLUSCA; MYTILUS; MYTILUS GALLOPROVINCIALIS; NEGIBACTERIA; POSIBACTERIA;

EID: 0033214630     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00654.x     Document Type: Article

References (30)

1. 1. Renwrantz, L. (1990) Internal defense system of Mytilus edulis. In Neurobiology of Mytilus edulis (Stephano, G., ed.), pp. 256-275. Manchester University Press, Manchester, UK.
2. 2. Bachère, E., Hervio, D. & Mialhe, E. (1991) Luminol-dependent chemiluminescence by hemocytes of two marine bivalves, Ostrea edulis and Crassostrea gigas. Dis. Aquat. Org. 11, 173-180.
3. 3. Hubert, F., Cooper, E.L. & Roch, Ph. (1997) Structure and differential target sensitivity of the stimulable cytotoxic complex from hemolymph of the Mediterranean mussel Mytilus galloprovincialis. Biochim. Biophys. Acta 1361, 29-41.
4. 4. Charlet, M., Chernysh, S., Philippe, H., Hétru, C., Hoffmann, J. & Bulet, P. (1996) Innate immunity. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J. Biol. Chem. 271, 21808-21813.
5. 5. Hubert, F., Noël, T. & Roch, Ph. (1996) A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis). Eur. J. Biochem. 240, 302-306.
6. 6. Hoffmann, J.A., Reichart, J.M. & Hetru, C. (1996) Innate immunity in higher insects. Curr. Opin. Immunol. 8, 8-13.
7. 7. Iwanaga, S., Kawabata, S.I. & Muta, T. (1998) New types of clotting factors and defense molecules found in horseshoe crab hemolymph: their structure and functions. J. Biochem. 123, 1-15.
8. 8. Bachère, E., Chagot, D. & Grizel, H. (1988) Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation. Dev. Comp. Immunol. 12, 549-559.
9. 9. Bulet, P., Cociancich, S., Dimarcq, J.L., Lambert, J., Reichhart, J.M., Hoffmann, D., Hétru, C. & Hoffmann, J.A. (1991) Insect immunity. Isolation from a coleopteran insect of a novel inducible antibacterial peptide and of new members of the insect defensin family. J. Biol. Chem. 266, 24520-24525.
10. 10. Hancock, B. (1997) Recently modified methods used by the Hancock Laboratory, http://www.interchg.ubc.ca/bobh/methods.htm.
11. 11. Fehlbaum, P., Balet, Ph., Michaut, L., Lagueux, M., Broekaert, W.F. & Hoffman, J.A. (1994) Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides. J. Biol. Chem. 269, 33159-33163.
12. 12. Gauthier, J.D. & Vasta, G.R. (1995) In vitro culture of the eastern oyster parasite Perkinsus marinus: optimization of the methodology. J. Invertebr. Pathol. 66, 156-168.
13. 13. Morvan, A., Iwanaga, S., Comps, M. & Bachère, E. (1997) In vitro activity of the Limulus antimicrobial peptide tachyplesin I on marine bivalve pathogens. J. Invertebr. Pathol. 69, 177-182.
14. 14. Kenchington, E.L.R., Landry, D. & Bird, C.J. (1995) Comparison of taxa of the mussel Mytilus (Bivalvia) by analysis of the nuclear small-subunit rRNA gene sequence. Can. J. Fish. Aquat. Sci. 52, 2613-2620.
15. 15. Nielsen, H., Engelbrecht, J., Brunak, S. & von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6.
16. 16. Breathnach, R. & Chambon, P. (1981) Organization and expression of eucaryotic split genes coding for proteins. Annu. Rev. Biochem. 50, 349-383.
17. 17. Mount, S.M. (1982) A catalogue of splice junction sequences. Nucleic Acids Res. 10, 459-472.
18. 18. Kreil, G. (1990) Processing of precursors by dipeptiylaminopeptidases: a case of molecular ticketing. Trends Biochem. Sci. 15, 23-26.
19. 19. Shigenaga, T., Muta, T., Toh, Y., Tokunaga, F. & Iwanaga, S. (1990) Antimicrobial tachyplesin peptide precursor cDNA cloning and cellular localization in the horseshoe crab (Tachypleus tridentatus). J. Biol. Chem. 265, 21350-21354.
20. 20. Destoumieux, D., Bulet, P., Loew, D., Van Dorsselaer, A., Rodriguez, J. & Bachère, E. (1997) Penaeidines, a new family of antimicrobial peptides isolated from the shrimp Peanaeus vannamei (Decapoda). J. Biol. Chem. 272, 28398-28406.
21. 21. Muta, T. & Iwanaga, S. (1996) The role of hemoplymph coagulation in innate immunity. Curr. Opin. Immunol. 8, 41-47.
22. 22. Saito, T., Kawabata, S.I., Shigenaga, T., Takayenoki, Y., Cho, J., Nakajima, H., Hirata, M. & Iwanaga, S. (1995) A novel big defensin identified in horseshoe crab hemocytes: isolation, amino acid sequence, and antibacterial activity. J. Biochem. 117, 1131-1137.
23. 23. Kawabata, S.I., Nagayama, R., Hirata, M., Shigenaga, T., Agarwala, K.L., Saito, T., Cho, J., Nakajima, H., Takagi, T. & Iwanaga, S. (1996) Tachycitin, a small granular component in horseshoe crab hemocytes, is an antimicrobial protein with chitin-binding activity. J. Biochem. 120, 1253-1260.
24. 24. Fehlbaum, P., Bulet, P., Chernysh, S., Briand, J.-P., Roussel, J.-P., Letellier, L., Hétru, C. & Hoffmann, J.A. (1996) Structure-activity analysis of thanatin, a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides. Proc. Natl Acad. Sci. USA 93, 1221-1225.
25. 25. Ehret-Sabatier, L., Loew, D., Goyffon, M., Fehbaum, P., Hoffmann, J.A., Van Dorsselaer, A. & Bulet, P. (1996) Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood. J. Biol. Chem. 271, 293537-229544.
26. 26. Simmaco, M., Mignogna, G., Barra, D. & Bossa, J.M. (1993) Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta. FEBS Lett. 324, 159-161.
27. 27. Broekaert, W.F., Terras, F.R.G., Cammue, B.P.A. & Osborn, R.W. (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108, 1353-1358.
28. 28. Tailor, R.H., Acland, D.P., Attenborough, S., Cammue, B.P., Evans, I.J., Osborn, R.W., Ray, J.A., Rees, S.B. & Broekaert, W.F. (1997) A novel family of small cysteine-rich antimiccrobial peptides from seed of Impatiens balsima is derived from a single precursor protein. J. Biol. Chem. 272, 24480-24487.
29. 29. Johnson, P.T., Stewart, J.E. & Arie, B. (1981) Histopathology of Aerococcus viridans var. homari infection (gaffkemia) in the lobster, Homarus americanus, and a comparison with histological reactions to a Gram-negative species, Pseudomonas perolens. J. Invertebr. Pathol. 38, 127-148.
30. 30. Friedman, C.S., Beattie, J.H., Elston, R.A. & Hedrick, R.P. (1991) Investigation of the relationship between the presence of a Gram-positive bacterial infection and summer mortality of the Pacific oyster, Crassostrea gigas Thunberg. Aquaculture 94, 1-15.