Molecular cloning, expression of a big defensin gene from bay scallop Argopecten irradians and the antimicrobial activity of its recombinant protein

Molecular Immunology

Volumn 44, Issue 4, 2007, Pages 360-368

  Zhao, Jianmin ^a,b   Song, Linsheng ^a   Li, Chenghua ^a   Ni, Duojiao ^a   Wu, Longtao ^a   Zhu, Ling ^a   Wang, Hao ^a   Xu, Wei ^a  

^a INSTITUTE OF OCEANOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Antimicrobial activity; Argopecten irradians; Big defensin; mRNA expression; Quantitative real time PCR; Recombinant protein

Indexed keywords

AMINO ACID; DEFENSIN; MESSENGER RNA; NUCLEOTIDE; POLYNUCLEOTIDE; POLYPEPTIDE; POLYPEPTIDE ANTIBIOTIC AGENT; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ANTIMICROBIAL ACTIVITY; ARGOPECTEN IRRADIANS; ARTICLE; BIOLOGICAL ACTIVITY; BLOOD CELL; BRANCHIOSTOMA BELCHERI TSINGTAUNESE; CONTROLLED STUDY; CRAB; EXPRESSED SEQUENCE TAG; FUNGICIDAL ACTIVITY; GENE AMPLIFICATION; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HEMOLYMPH; IMMUNE RESPONSE; INNATE IMMUNITY; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; POLYADENYLATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SCALLOP; SEQUENCE HOMOLOGY; SHRIMP; TACHYPLEUS TRIDENTATUS; VIBRIO; VIBRIO ANGUILARUM;

AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; BACTERIA; BASE SEQUENCE; CLONING, MOLECULAR; DEFENSINS; DNA, COMPLEMENTARY; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PECTINIDAE; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

ARGOPECTEN IRRADIANS; BACTERIA (MICROORGANISMS); BRANCHIOSTOMA BELCHERI TSINGTAUNESE; NEGIBACTERIA; PECTINIDAE; POSIBACTERIA; TACHYPLEUS TRIDENTATUS; VIBRIO;

EID: 33747829917     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molimm.2006.02.025     Document Type: Article

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