Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas

Developmental and Comparative Immunology

Volumn 31, Issue 4, 2007, Pages 332-339

  Gonzalez, M ^a   Gueguen, Y ^a   Desserre, G ^a   de Lorgeril, J ^a   Romestand, B ^a   Bachere E ^a  

^a IFREMER   (France)

Author keywords

Antimicrobial peptide; Defensin; Hemocytes; Marine invertebrate; Mollusc bivalve; Pacific oyster

Indexed keywords

DEFENSIN; DEFENSIN 1; DEFENSIN 2; ISOPROTEIN;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTHROPOD; ARTICLE; BLOOD CELL; CELL MIGRATION; CONTROLLED STUDY; GILL; MOLECULAR CLONING; MOLLUSC; NONHUMAN; NUCLEOTIDE SEQUENCE; OYSTER; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FAMILY; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

ARTHROPODA; BACTERIA (MICROORGANISMS); BIVALVIA; CRASSOSTREA GIGAS; INVERTEBRATA; MOLLUSCA; OSTREIDAE;

EID: 33845601729     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2006.07.006     Document Type: Article

References (19)

1. Bachère E., Gueguen Y., Gonzalez M., de Lorgeril J., Garnier J., and Romestand B. Insights into the antimicrobial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunol Rev 198 (2004) 149-168
2. Bulet P., Stocklin R., and Menin L. Anti-microbial peptides: from invertebrates to vertebrates. Immunol Rev 198 (2004) 169-184
3. Boulanger N., Lowenberger C., Volf P., Ursic R., Sigutova L., Sabatier L., et al. Characterization of a defensin from the sand fly Phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite Leishmania major. Infect Immun 72 (2004) 7140-7146
4. Harder J., Bartels J., Christophers E., and Schroder J.M. Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276 (2001) 5707-5713
5. Dimarcq J.-L., Bulet P., Hétru C., and Hoffmann J.A. Cysteine-rich antimicrobial peptides in invertebrates. Biopolymers 47 (1998) 465-477
6. Iijima R., Kisugi J., and Yamazaki M. A novel antimicrobial peptide from the sea hare Dolabella auricularia. Dev Comp Immunol 27 (2003) 305-311
7. Charlet M., Chernysh S., Philippe H., Hétru C., Hoffmann J.A., and Bulet P. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J. Biol. Chem 271 (1996) 21808-21813
8. Hubert F., Noël T., and Roch P. A member of the arthropod defensin family from the edible Mediterranean mussels (Mytilus galloprovincialis). Eur J Biochem 240 (1996) 302-306
9. Mitta G., Vandenbulcke F., Hubert F., and Roch P. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci 112 (1999) 4233-4242
10. Mitta G., Vandenbulcke F., Hubert F., Salzet M., and Roch P. Involvement of mytilins in mussel antimicrobial defense. J Biol Chem 275 (2000) 12954-12962
11. Seo J.K., Crawford J.M., Stone K.L., and Noga E.J. Purification of a novel arthropod defensin from the American oyster, Crassostrea virginica. Biochem Biophys Res Commun 338 (2005) 1998-2004
12. Gueguen Y., Herpin A., Aumelas A., Garnier J., Fievet J., Escoubas J.M., et al. Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression. J Biol Chem 281 (2006) 313-323
13. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 (2001) 402-408
14. Tincu J.A., and Taylor S.W. Antimicrobial peptides from marine invertebrates. Antimicrob Agents Chemother 48 (2004) 3645-3654
15. Yang Y.S., Mitta G., Chavanieu A., Calas B., Sanchez J.F., Roch P., et al. Solution structure and activity of the synthetic four-disulfide bond mediterranean mussel defensin (MGD-1). Biochemistry 39 (2000) 14436-14447
16. Froy O., and Gurevitz M. Arthropod and mollusk defensins evolution by exon-shuffling. Trends Genet 19 (2003) 684-687
17. Mygind P.H., Fischer R.L., Schnorr K.M., Hansen M.T., Sonksen C.P., Ludvigsen S., et al. Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature 437 (2005) 975-980
18. Gueguen Y., Cadoret J.P., Flament D., Barreau-Roumiguiere C., Girardot A.L., Garnier J., et al. Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas. Gene 16 (2003) 139-145
19. Hoffmann J.A., Reichhart J.M., and Hetru C. Innate immunity in higher insects. Curr Opin Immunol 8 (1996) 8-13