Big defensins, a diverse family of antimicrobial peptides that follows different patterns of expression in hemocytes of the oyster crassostrea gigas

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Rosa, Rafael D ^a,b   Santini, Adrien ^a   Fievet, Julie ^a   Bulet, Philippe ^c,d   Destoumieux Garzón, Delphine ^b   Bachère, Evelyne ^a  

^a IFREMER   (France)

^b CNRS   (France)

^c PSL RESEARCH UNIVERSITY   (France)

^d AGIM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BETA DEFENSIN; BIG DEFENSIN 1 PROTEIN; BIG DEFENSIN 2 PROTEIN; BIG DEFENSIN 3 PROTEIN; DEFENSIN; GLUTAMINE; POLYPEPTIDE ANTIBIOTIC AGENT; PYROGLUTAMIC ACID; UNCLASSIFIED DRUG; TRANSCRIPTOME;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BLOOD CELL; CARBOXY TERMINAL SEQUENCE; CG BIGDEF1 GENE; CG BIGDEF2 GENE; CG BIGDEF3 GENE; CONTROLLED STUDY; CYCLIZATION; EXON; GENE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC CODE; GENETIC VARIABILITY; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OYSTER; PHYLOGENY; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; SEQUENCE ANALYSIS; UPREGULATION; VIBRIOSIS; ANIMAL; CHEMISTRY; CYTOLOGY; EXPRESSED SEQUENCE TAG; GENE EXPRESSION PROFILING; GENETIC DATABASE; GENETICS; GENOMICS; IMMUNOLOGY; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; MULTIGENE FAMILY; PATHOGENICITY; PROTEIN TERTIARY STRUCTURE; VIBRIO;

BACTERIA (MICROORGANISMS); CRASSOSTREA GIGAS; FUNGI; INVERTEBRATA; MOLLUSCA; OSTREIDAE; VERTEBRATA; VIBRIO;

AMINO ACID SEQUENCE; ANIMALS; CRASSOSTREA; DATABASES, GENETIC; DEFENSINS; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION PROFILING; GENETIC VARIATION; GENOMICS; HEMOCYTES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PROTEIN STRUCTURE, TERTIARY; TRANSCRIPTOME; UP-REGULATION; VIBRIO; VIBRIO INFECTIONS;

EID: 80053261618     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025594     Document Type: Article

References (43)

1. Kawabata S, (2011) Immunocompetent molecules and their response network in horseshoe crabs. Adv Exp Med Biol 708: 122-136.
2. Saito T, Kawabata S, Shigenaga T, Takayenoki Y, Cho J, et al. (1995) A novel big defensin identified in horseshoe crab hemocytes: Isolation, amino acid sequence, and antibacterial activity. J Biochem 117 Tokyo: 1131-1137.
3. Kouno T, Fujitani N, Mizuguchi M, Osaki T, Nishimura S, et al. (2008) A novel beta-defensin structure: a potential strategy of big defensin for overcoming resistance by Gram-positive bacteria. Biochemistry 47: 10611-10619.
4. Selsted ME, Ouellette AJ, (2005) Mammalian defensins in the antimicrobial immune response. Nat Immunol 6: 551-557.
5. Martin E, Ganz T, Lehrer RI, (1995) Defensins and other endogenous peptide antibiotics of vertebrates. J Leukoc Biol 58: 128-136.
6. Bulet P, Stöcklin R, (2005) Insect antimicrobial peptides: structures, properties and gene regulation. Protein Pept Lett 12: 3-11.
7. Yang YS, Mitta G, Chavanieu A, Calas B, Sanchez JF, et al. (2000) Solution structure and activity of the synthetic four-disulfide bond Mediterranean mussel defensin (MGD-1). Biochemistry 39: 14436-14447.
8. Gueguen Y, Herpin A, Aumelas A, Garnier J, Fievet J, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression. J Biol Chem 281: 313-323.
9. Perrigault M, Tanguy A, Allam B, (2009) Identification and expression of differentially expressed genes in the hard clam, Mercenaria mercenaria, in response to quahog parasite unknown (QPX). BMC Genomics 10: 377-394.
10. Fleury E, Huvet A, Lelong C, de Lorgeril J, Boulo V, et al. (2009) Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase. BMC Genomics 10: 341-356.
11. Zhao J, Li C, Chen A, Li L, Su X, et al. (2010) Molecular characterization of a novel big defensin from clam Venerupis philippinarum. PLoS ONE 5: e13480.
12. Zhao J, Song L, Li C, Ni D, Wu L, et al. (2007) Molecular cloning, expression of a big defensin gene from bay scallop Argopecten irradians and the antimicrobial activity of its recombinant protein. Mol Immunol 44: 360-368.
13. Schmitt P, Gueguen Y, Desmarais E, Bachère E, de Lorgeril J, (2010) Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas. BMC Evol Biol 10: 23.
14. Cuthbertson BJ, Deterding LJ, Williams JG, Tomer KB, Etienne K, et al. (2008) Diversity in penaeidin antimicrobial peptide form and function. Dev Comp Immunol 32: 167-181.
15. Gonzalez M, Gueguen Y, Desserre G, de Lorgeril J, Romestand B, et al. (2007) Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas. Dev Comp Immunol 31: 332-339.
16. Gueguen Y, Romestand B, Fievet J, Schmitt P, Destoumieux-Garzón D, et al. (2009) Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin. Mol Immunol 46: 516-522.
17. Schmitt P, Wilmes M, Pugnière M, Aumelas A, Bachère E, et al. (2010) Insight into invertebrate defensin mechanism of action: oyster defensins inhibit peptidoglycan biosynthesis by binding to lipid II. J Biol Chem 285: 29208-29216.
18. de Lorgeril J, Zenagui R, Rosa RD, Piquemal D, Bachère E, (2011) Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by Digital Gene Expression analysis. PLoS ONE 5: e23142.
19. Nagle GT, Garcia AT, Gorham EL, Knock SL, van Heumen WR, et al. (1995) Molecular cloning and cellular localization of a furin-like prohormone convertase from the atrial gland of Aplysia. DNA Cell Biol 14: 431-443.
20. Spijker S, Smit AB, Sharp-Baker HE, Van Elk R, Van Kesteren ER, et al. (1999) Family of prohormone convertases in Lymnaea: characterization of two alternatively spliced furin-like transcripts and cell-specific regulation of their expression. J Neurobiol 41: 399-413.
21. Gay M, Renault T, Pons AM, Le Roux F, (2004) Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations. Dis Aquat Organ 62: 65-74.
22. Thompson FL, Thompson CC, Swings J, (2003) Vibrio tasmaniensis sp. nov., isolated from Atlantic salmon (Salmo salar L.). Syst Appl Microbiol 26: 65-69.
23. Valore EV, Ganz T, (1992) Posttranslational processing of defensins in immature human myeloid cells. Blood 79: 1538-1544.
24. Rabel D, Charlet M, Ehret-Sabatier L, Cavicchioli L, Cudic M, et al. (2004) Primary structure and in vitro antibacterial properties of the Drosophila melanogaster attacin C Pro-domain. J Biol Chem 279: 14853-14859.
25. Huang Y, Huang J, Chen Y, (2010) Alpha-helical cationic antimicrobial peptides: relationships of structure and function. Protein Cell 1: 143-152.
26. Keren H, Lev-Maor G, Ast G, (2010) Alternative splicing and evolution: diversification, exon definition and function. Nat Rev Genet 11: 345-355.
27. Hollox EJ, Armour JA, Barber JC, (2003) Extensive normal copy number variation of a beta-defensin antimicrobial-gene cluster. Am J Hum Genet 73: 591-600.
28. Lazzaro BP, Sceurman BK, Clark AG, (2004) Genetic basis of natural variation in D. melanogaster antibacterial immunity. Science 303: 1873-1876.
29. Tennessen JA, Woodhams DC, Chaurand P, Reinert LK, Billheimer D, et al. (2009) Variations in the expressed antimicrobial peptide repertoire of northern leopard frog (Rana pipiens) populations suggest intraspecies differences in resistance to pathogens. Dev Comp Immunol 33: 1247-1257.
30. Abu Bakar S, Hollox EJ, Armour JA, (2009) Allelic recombination between distinct genomic locations generates copy number diversity in human beta-defensins. Proc Natl Acad Sci U S A 106: 853-858.
31. Gonzalez M, Gueguen Y, Destoumieux-Garzón D, Romestand B, Fievet J, et al. (2007) Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI. Proc Natl Acad Sci U S A 104: 17759-17764.
32. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, et al. (1999) Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol 163: 6718-6724.
33. Thompson FL, Iida T, Swings J, (2004) Biodiversity of vibrios. Microbiol Mol Biol Rev 68: 403-431.
34. Mukherjee S, Vaishnava S, Hooper LV, (2008) Multi-layered regulation of intestinal antimicrobial defense. Cell Mol Life Sci 65: 3019-3027.
35. Garnier M, Labreuche Y, Nicolas JL, (2008) Molecular and phenotypic characterization of Vibrio aestuarianus subsp. francensis subsp. nov., a pathogen of the oyster Crassostrea gigas. Syst Appl Microbiol 31: 358-365.
36. Destoumieux D, Muñoz M, Bulet P, Bachère E, (2000) Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda). Cell Mol Life Sci 57: 1260-1271.
37. Rosa RD, Barracco MA, (2010) Antimicrobial peptides in crustaceans. Inv Surv J 7: 262-284.
38. Piquemal D, Commes T, Manchon L, Lejeune M, Ferraz C, et al. (2002) Transcriptome analysis of monocytic leukemia cell differentiation. Genomics 80: 361-371.
39. Duckert P, Brunak S, Blom N, (2004) Prediction of proprotein convertase cleavage sites. Protein Eng Des Sel 17: 107-112.
40. Tamura K, Dudley J, Nei M, Kumar S, (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599.
41. Duperthuy M, Binesse J, Le Roux F, Romestand B, Caro A, et al. (2010) The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas. Environ Microbiol 12: 951-963.
42. Pfaffl MW, (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45.
43. Muñoz M, Vandenbulcke F, Saulnier D, Bachère E, (2002) Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimps. Eur J Biochem 269: 2678-2689.