Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway

Journal of Biological Chemistry

Volumn 288, Issue 1, 2013, Pages 297-305

  Moore, Simon J ^a   Biedendieck, Rebekka ^a,b   Lawrence, Andrew D ^a   Deery, Evelyne ^a   Howard, Mark J ^a   Rigby, Stephen E J ^c   Warren, Martin J ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS MEGATERIUM; BIOSYNTHETIC PATHWAY; C-TERMINAL EXTENSIONS; COBALT ION; HIGH YIELD; MACROCYCLES; MEGATERIUM; OXIDATION STATE; PURIFIED ENZYME; RING CONTRACTION; SEQUENCE SIMILARITY; VITAMIN B;

BACTERIOLOGY; BIOCHEMISTRY; BIOSYNTHESIS; COBALT; MAGNETIC RESONANCE; PARAMAGNETISM; PURIFICATION;

ENZYMES;

BACTERIAL ENZYME; COBALAMIN; COBALT; COBALT DERIVATIVE; COBALT PRECORRIN 3; CONTRACTASE; MACROCYCLIC COMPOUND; NITRITE REDUCTASE; SULFITE REDUCTASE; UNCLASSIFIED DRUG; COBAMAMIDE; CYANOCOBALAMIN; DITHIOTHREITOL; IRON SULFUR PROTEIN; OXYGEN; RECOMBINANT PROTEIN;

AEROBIC BACTERIUM; ARTICLE; BACILLUS MEGATERIUM; CARBOXY TERMINAL SEQUENCE; CATALYSIS; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; GRAM POSITIVE BACTERIUM; NONHUMAN; OXIDATION; PRIORITY JOURNAL; SOLUBILITY; BIOSYNTHESIS; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; GENE EXPRESSION REGULATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SITE DIRECTED MUTAGENESIS;

BACILLUS MEGATERIUM; BACTERIA (MICROORGANISMS); POSIBACTERIA;

BACILLUS MEGATERIUM; BIOSYNTHETIC PATHWAYS; CATALYSIS; CLONING, MOLECULAR; COBALT; COBAMIDES; DITHIOTHREITOL; ELECTRON SPIN RESONANCE SPECTROSCOPY; GENE EXPRESSION REGULATION, ENZYMOLOGIC; IRON-SULFUR PROTEINS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; OXYGEN; RECOMBINANT PROTEINS; SOLUBILITY; VITAMIN B 12;

MLCS; MLOWN;

EID: 84872076063     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.422535     Document Type: Article

References (29)

1. Minot, G. R., and Murphy, W. P. (1926) Treatment of pernicious anemia by a special diet. JAMA 87, 470
2. Warren, M. J., and Scott, A. I. (1990) Tetrapyrrole assembly and modification into the ligands of biologically functional cofactors. Trends Biochem. Sci. 15, 486-491
3. Heinemann, I. U., Jahn, M., and Jahn, D. (2008) The biochemistry of heme biosynthesis. Arch. Biochem. Biophys. 474, 238-251
4. Scott, A. I., Roessner, C. A., Stolowich, N. J., Spencer, J. B., Min, C., and Ozaki, S. I. (1993) Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group. FEBS Lett. 331, 105-108
5. 12. Factor IV, a new intermediate in the anaerobic pathway. Proc. Natl. Acad. Sci. U.S.A. 93, 14316-14319
6. 12). Nat. Prod Rep. 19, 390-412
7. 12. Mode of incorporation of factor III into cobyrinic acid. Proc. Natl. Acad. Sci. U.S.A. 78, 9-10
8. Raux, E., Leech, H. K., Beck, R., Schubert, H. L., Santander, P. J., Roessner, C. A., Scott, A. I., Martens, J. H., Jahn, D., Thermes, C., Rambach, A., and Warren, M. J. (2003) Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium. Biochem. J. 370, 505-516 (Pubitemid 36315139)
9. 12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus. J. Biol. Chem. 282, 23957-23969
10. 12) biosynthetic pathway, contains an Fe-S center and a mononuclear nonheme iron center. J. Biol. Chem. 284, 4796-4805
11. Frank, S., Brindley, A. A., Deery, E., Heathcote, P., Lawrence, A. D., Leech, H. K., Pickersgill, R. W., and Warren, M. J. (2005) Anaerobic synthesis of vitamin B12. Characterization of the early steps in the pathway. Biochem. Soc. Trans. 33, 811-814 (Pubitemid 41160869)
12. Schubert, H. L., Rose, R. S., Leech, H. K., Brindley, A. A., Hill, C. P., Rigby, S. E., and Warren, M. J. (2008) Structure and function of SirC from Bacillus megaterium. A metal-binding precorrin-2 dehydrogenase. Biochem. J. 415, 257-263
13. Romão, C. V., Ladakis, D., Lobo, S. A., Carrondo, M. A., Brindley, A. A., Deery, E., Matias, P. M., Pickersgill, R. W., Saraiva, L. M., and Warren, M. J. (2011) Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization. Proc. Natl. Acad. Sci. U.S.A. 108, 97-102
14. Spencer, P., Stolowich, N. J., Sumner, L. W., and Scott, A. I. (1998) Definition of the redox states of cobalt-precorrinoids. Investigation of the substrate and redox specificity of CbiL from Salmonella typhimurium. Biochemistry 37, 14917-14927 (Pubitemid 28487601)
15. Santander, P. J., Roessner, C. A., Stolowich, N. J., Holderman, M. T., and Scott, A. I. (1997) How corrinoids are synthesized without oxygen. Nature's first pathway to vitamin B12. Chem. Biol. 4, 659-666 (Pubitemid 27426623)
16. Kozbial, P. Z., and Mushegian, A. R. (2005) Natural history of S-adenosyl-methionine- binding proteins. BMC Struct. Biol. 5, 19
17. Raux, E., Lanois, A., Rambach, A., Warren, M. J., and Thermes, C. (1998) Cobalamin (vitamin B12) biosynthesis. Functional characterization of the Bacillus megaterium cbi genes required to convert uroporphyrinogen III into cobyrinic acid a,c-diamide. Biochem. J. 335, 167-173
18. Barg, H., Malten, M., Jahn, M., and Jahn, D. (2005) in Microbial Processes and Products (Barredo, J. L., ed), 1st Ed., pp. 165-184, Humana Press, Totowa, NJ
19. Raux, E., Lanois, A., Warren, M. J., Rambach, A., and Thermes, C. (1998) Cobalamin (vitamin B12) biosynthesis. Identification and characterization of a Bacillus megaterium cobI operon. Biochem. J. 335, 159-166
20. Biedendieck, R., Yang, Y., Deckwer, W. D., Malten, M., and Jahn, D. (2007) Plasmid system for the intracellular production and purification of affin-ity- tagged proteins in Bacillus megaterium. Biotechnol. Bioeng 96, 525-537 (Pubitemid 46232358)
21. Ollagnier-De Choudens, S., Sanakis, Y., Hewitson, K. S., Roach, P., Baldwin, J. E., Münck, E., and Fontecave, M. (2000) Iron-sulfur center of biotin synthase and lipoate synthase. Biochemistry 39, 4165-4173 (Pubitemid 30210396)
22. Riemer, J., Hoepken, H. H., Czerwinska, H., Robinson, S. R., and Dringen, R. (2004) Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal. Biochem. 331, 370-375 (Pubitemid 38953089)
23. 12). Characterisation of the anaerobic pathway in Methanobacter thermoautotrophicus. Ph.D. Thesis, Queen Mary University of London
24. L. Evidence for a 4Fe-4S center housed within an MXCXXC motif. J. Biol. Chem. 278, 41900-41907
25. Saha, K., Webb, M. E., Rigby, S. E., Leech, H. K., Warren, M. J., and Smith, A. G. (2012) Characterisation of the evolutionary conserved iron-sulphur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana. Biochem. J. 444, 227-237
26. Dailey, H. A., Finnegan, M. G., and Johnson, M. K. (1994) Human ferrochelatase is an iron-sulfur protein. Biochemistry 33, 403-407 (Pubitemid 24048081)
27. Brindley, A. A., Raux, E., Leech, H. K., Schubert, H. L., and Warren, M. J. (2003) A story of chelatase evolution. Identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaea. J. Biol. Chem. 278, 22388-22395 (Pubitemid 36830289)
28. Roessner, C. A., Santander, P. J., and Scott, A. I. (2001) Multiple biosynthetic pathways for vitamin B12. Variations on a central theme. Vitam Horm. 61, 267-297 (Pubitemid 33805698)
29. Roessner, C. A., and Scott, A. I. (2006) Fine-tuning our knowledge of the anaerobic route to cobalamin (vitamin B12). J. Bacteriol. 188, 7331-7334 (Pubitemid 44646231)