Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium

Biotechnology and Bioengineering

Volumn 96, Issue 3, 2007, Pages 525-537

  Biedendieck, Rebekka ^a   Yang, Yang ^a   Deckwer, Wolf Dieter ^a   Malten, Marco ^a   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Affinity tags; Bacillus megaterium; Fed batch cultivation; Green fluorescent protein; Recombinant protein production

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELL CULTURE; GLUCOSE; GROWTH KINETICS; PROTEINS; VIRUSES;

AFFINITY TAGS; BACILLUS MEGATERIUM; FED BATCH CULTIVATION; GREEN FLUORESCENT PROTEIN (GFP); PLASMID SYSTEM; RECOMBINANT PROTEIN PRODUCTION; TOBACCO ETCH VIRUS (TEV);

BACTERIA;

BACTERIAL PROTEIN; BLOOD CLOTTING FACTOR 10A; GLUCOSE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN TAG; PROTEINASE; VIRUS ENZYME; XYLOSE; XYLOSE ISOMERASE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS MEGATERIUM; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION; GENE VECTOR; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN PURIFICATION;

BACILLUS MEGATERIUM; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYTOPLASM; ENDOPEPTIDASES; FACTOR XA; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTIDINE; PLASMIDS; RECOMBINANT FUSION PROTEINS;

BACILLUS MEGATERIUM; TOBACCO ETCH VIRUS;

EID: 33846913266     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21145     Document Type: Article

References (25)

1. Barg H, Malten M, Jahn M, Jahn D. 2005. Protein and vitamin production in Bacillus megaterium. In: Barredo JL, editor. Microbial processes and products. Totowa: Humana Press Inc. p165-184.
2. Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173 (1 Spec No.): 33-38.
3. Grote A, Hiller K, Scheer M, Munch R, Nortemann B, Hempel DC, Jahn D. 2005. JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Res 33 (Web Server issue):W526-31.
4. Hollmann R, Deckwer WP. 2004. Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation. J Biotechnol 111(1):89-96.
5. Inouye S, Tsuji FI. 1994. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett 341(2-3):277-280.
6. Kapust RB, Tozser J, Copeland TD, Waugh DS. 2002. The P1′ specificity of tobacco etch virus protease. Biochem Biophys Res Commun 294(5):949-955.
7. Leech HK, Raux E, McLean KJ, Munro AW, Robinson NJ, Borrelly GP, Malten M, Jahn D, Rigby SE, Heathcote P and others. 2003. Characterization of the cobaltochelatase CbiXL: Evidence for a 4Fe-4S center housed within an MXCXXC motif. J Biol Chem 278(43):41900-41907.
8. Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. 2005. Comparison of affinity tags for protein purification. Protein Expr Purif 41(1):98-105.
9. Makrides SC. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60(3):512-538.
10. Malten M, Hollmann R, Deckwer WD, Jahn D. 2005. Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol Bioeng 89(2):206-218.
11. Malten M, Biedendieck R, Gamer M, Drews AC, Stammen S, Buchholz K, Dijkhuizen L, Jahn D. 2006. A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium. Appl Environ Microbiol 72(2):1677-1679.
12. Nagai K, Thogersen HC. 1984. Generation of beta-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli. Nature 309(5971):810-812.
13. Porath J, Carlsson J, Olsson I, Belfrage G. 1975. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258(5536):598-599.
14. Reischer H, Schotola I, Striedner G, Potschacher F, Bayer K. 2004. Evaluation of the GFP signal and its aptitude for novel on-line monitoring strategies of recombinant fermentation processes. J Biotechnol 108(2):115-125.
15. Rygus T, Hillen W. 1992. Catabolite repression of the xyl operon in Bacillus megaterium. J Bacteriol 174(9):3049-3055.
16. Rygus T, Scheler A, Allmansberger R, Hillen W. 1991. Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155(6):535-542.
17. Sambrook J, Russell DW. 2001. Molecular cloning: A laboratory manual. Woodbury, USA: Cold Spring Harbor Laboratory Press. 999 p.
18. Schmidt TG, Koepke J, Frank R, Skerra A. 1996. Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J Mol Biol 255(5):753-766.
19. Scholz O, Thiel A, Hillen W, Niederweis M. 2000. Quantitative analysis of gene expression with an improved green fluorescent protein. Eur J Biochem 267(6):1565-1570.
20. Terpe K. 2003. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 60(5):523-533.
21. van Hijum SA, Bonting K, van der Maarel MJ, Dijkhuizen L. 2001. Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced. FEMS Microbiol Lett 205(2):323-328.
22. Vary PS. 1992. Development of Genetic Engineering in Bacillus megaterium. In: Doi R, McGloughlin M, editors. Biology of Bacilli: Application to industry. Boston: Butterworths-Heinemann. p 251-310.
23. Waugh DS. 2005. Making the most of affinity tags. Trends Biotechnol 23(6):316-320.
24. Wittchen KD, Meinhardt F. 1995. Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl Microbiol Biotechnol 42(6):871-877.
25. Yoon SM, Kim SC, Kim JH. 1994. Identification of inhibitory metabolites in high cell density culture of recombinant Bacillus megaterium PCK108. Biotechnol Lett 16(10):1011-1014.