Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium

Biochemical Journal

Volumn 370, Issue 2, 2003, Pages 505-516

  Raux, Evelyne ^a   Leech, Helen K ^a   Beck, Richard ^a   Schubert, Heidi L ^b   Santander, Patricio J ^c   Roessner, Charles A ^c   Scott, A Ian ^c   Martens, Jan H ^d   Jahn, Dieter ^d   Thermes, Claude ^e   Rambach, Alain ^f   Warren, Martin J ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^c TEXAS A AND M UNIVERSITY   (United States)

^d TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^e CNRS   (France)

^f CHROMagar   (France)

Author keywords

CbiX; SirA; SirB; SirC; Sirohydrochlorin

Indexed keywords

BIOSYNTHESIS; CATALYSIS; CHELATION; DEHYDROGENATION; ESCHERICHIA COLI; GENES;

BACILLUS MEGATERIUM;

ENZYMES;

COBALAMIN; GLUTAMINE; HISTIDINE; METAL ION; PORPHOBILINOGEN DEAMINASE; PRECORRIN 2; PROTEIN; PROTEIN SIROHAEM; SIROHYDROCHLORIN; TRANSFER RNA; UNCLASSIFIED DRUG; UROPORPHYRINOGEN III SYNTHASE;

ARTICLE; BACILLUS MEGATERIUM; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENETIC CODE; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; BACILLUS MEGATERIUM; ESCHERICHIA COLI; HEME; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; SALMONELLA TYPHIMURIUM; UROPORPHYRINS; VITAMIN B 12;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA; POSIBACTERIA; SALMONELLA; SALMONELLA ENTERICA; SEIRA;

EID: 0037337451     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021443     Document Type: Article

References (35)

1. 12): a bacterial conundrum. Cell. Mol. Life Sci. 57, 1880-1893
2. Walker, C. J. and Willows, R. D. (1997) Mechanism and regulation of Mg-chelatase. Biochem. J. 327, 321-333
3. 12; insights into biosynthesis's Mount improbable. Bioorg. Chem. 27, 100-118
4. Vary, P. (1994) Prime time for Bacillus megaterium. Microbiology 140, 1001-1013
5. Raux, E., McVeigh, T., Peters, S. E., Leustek, T. and Warren, M. J. (1999) The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis. Biochem. J. 338, 701-708
6. Spencer, J. B., Stolowich, N. J., Roessner, C. A. and Scott, A. I. (1993) The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase. FEBS Lett. 335, 57-60
7. Warren, M. J., Bolt, E. L., Roessner, C. A., Scott, A. I., Spencer, J. B. and Woodcock, S. C. (1994) Gene dissection demonstrates that the Escherichia coli cysG gene encodes a multifunctional protein. Biochem. J. 302, 837-844
8. Schubert, H. L., Wilson, K. S., Raux, E., Woodcock, S. C. and Warren, M. J. (1998) The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase. Nat. Struct. Biol. 5, 585-592
9. Schubert, H. L., Raux, E., Brindley, A. A., Leech, H. K., Wilson, K. S., Hill, C. P. and Warren, M. J. (2002) The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase. EMBO J. 21, 1-8
10. Johansson, P. and Hederstedt, L. (1999) Organization of genes for tetrapyrrole biosynthesis in gram-positive bacteria. Microbiology 145, 529-538
11. 12) biosynthesis: functional characterization of the Bacillus megaterium cbi genes required to convert uroporphyrinogen III into cobyrinic acid a,c-diamide. Biochem. J. 335, 167-173
12. 12) biosynthesis: identification and characterization of a Bacillus megaterium cobl operon. Biochem. J. 335, 159-166
13. 12) and siroheme biosynthesis. J. Bacteriol. 179, 3202-3212
14. Schubert, H. L., Raux, E., Wilson, K. S. and Warren, M. J. (1999) Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis. Biochemistry 38, 10660-10669
15. 12) biosynthetic genes: functional studies in S. typhimurium and Escherichia coli. J. Bacteriol. 178, 753-767
16. Hansson, M., Rutberg, L., Schroder, I. and Hederstedt, L. (1991) The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III. J. Bacteriol. 173, 2590-2599
17. Robin, C., Blanche, F., Cauchois, L., Cameron, B., Couder, M. and Crouzet, J. (1991) Primary structure, expression in Escherichia coli, and properties of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Bacillus megaterium. J. Bacteriol. 173, 4893-4896
18. Jordan, P. M. and Berry, A. (1981) Mechanism of action of porphobilinogen deaminase. The participation of stable enzyme substrate covalent intermediates between porphobilinogen and the porphobilinogen deaminase from Rhodopseudomonas spheroides. Biochem. J. 195, 177-181
19. Warren, M. J. and Jordan, P. M. (1988) Investigation into the nature of substrate binding to the dipyrromethane cofactor of Escherichia coli porphobilinogen deaminase. Biochemistry 27, 9020-9030
20. Jordan, P. M. (1991) The biosynthesis of 5-aminolaevulinic acid and its transformation into uroporphyrinogen III, in 'Biosynthesis of Tetrapyrroles' (Jordan, P. M., ed.), pp. 1-59, Elsevier, Amsterdam
21. Griffiths, L. A. and Cole, J. A. (1987) Lack of redox control of the anaerobicallyinduced nirB+ gene of Escherichia coli K-12. Arch. Microbiol. 147, 364-369
22. Blanche, F., Debussche, L., Thibaut, D., Crouzet, J. and Cameron, B. (1989) Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans. J. Bacteriol. 171, 4222-4231
23. Crouzet, J., Cauchois, L., Blanche, F., Debussche, L., Thibaut, D., Rouyez, M. C., Rigault, S., Mayaux, J. F. and Cameron, B. (1990) Nucleotide sequence of a Pseudomonas denitrificans 5.4 kb DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase. J. Bacteriol. 172, 5968-5979
24. Warren, M. J., Stolowich, N. J., Santander, P. J., Roessner, C. A., Sowa, B. A. and Scott, A. I. (1990) Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase. FEBS Lett. 261, 76-80
25. 12. J. Am. Chem. Soc. 100, 316-318
26. Spencer, P., Stolowich, N. J., Sumner, L. W. and Scott, A. I. (1998) Definition of the redox states of cobalt-precorrinoids: investigation of the substrate and redox specificity of CbiL from Salmonella typhimurium. Biochemistry 37, 14917-14927
27. Battersby, A. R. and Sheng, Z.-C. (1982) Preparation and spectroscopic properties of Co(III)-isobacteriochlorins: Relationship to the cobalt-containing proteins from Desulphovibrio gigas and D. desulphuricans. J. Chem. Soc., Chem. Commun., 1393-1394
28. 12. Chem. Biol. 4, 659-666
29. 12: Factor IV, a new intermediate in the anaerobic pathway. Proc. Natl. Acad. Sci. U.S.A. 93, 14316-14319
30. Outten, C. E. and O'Halloran, T. V. (2001) Femtomolar sensitivity of metalloregutatory proteins controlling zinc homeostasis. Science (Washington, D.C.) 292, 2488-2492
31. Rey, L., Imperial, J., Palacios, J. M. and Ruiz-Argueso, T. (1994) Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis. J. Bacteriol. 176, 6066-6073
32. Fu, C., Olson, J. W. and Maier, R. J. (1995) HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer. Proc. Natl. Acad. Sci. U.S.A. 92, 2333-2337
33. Kerby, R. L., Ludden, P. W. and Roberts, G. P. (1997) In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ. J. Bacteriol. 179, 2259-2266
34. Olson, J. W., Fu, C. and Maier, R. J. (1997) The HypB protein from Bradyrhizobium japonicum can store nickel and is required for the nickel-dependent transcriptional regulation of hydrogenase. Mol. Microbiol. 24, 119-128
35. Kraulis, P. J. (1991) MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J. Appl. Cryst. 24, 946-950