A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration

Applied Microbiology and Biotechnology

Volumn 72, Issue 3, 2006, Pages 514-520

  Mouri, Tsuyoshi ^a   Michizoe, Junji ^a   Ichinose, Hirofumi ^b   Kamiya, Noriho ^a   Goto, Masahiro ^a,b  

^a KYUSHU UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450CAM MONOOXYGENASE; ELECTRON TRANSFER; GLYCEROL DEHYDROGENASE (GLD); SOIL BACTERIUM;

BACTERIA; BIOCATALYSTS; CELLS; ELECTRON TRANSITIONS; ENZYMES; HYDROXYLATION; OXYGENATORS;

ESCHERICHIA COLI;

CAMPHOR 5 MONOOXYGENASE; GLYCEROL DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

COLIFORM BACTERIUM; CYTOCHROME; ENZYME ACTIVITY; GENE EXPRESSION; NUCLEIC ACID; OXYGENATION; PROTEIN;

ARTICLE; BIOCATALYST; CATALYSIS; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENETIC RECOMBINATION; HYDROXYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION;

CAMPHOR; CAMPHOR 5-MONOOXYGENASE; COENZYMES; ESCHERICHIA COLI; HYDROXYLATION; NAD; RECOMBINANT PROTEINS; SUGAR ALCOHOL DEHYDROGENASES;

BACTERIA (MICROORGANISMS); DRYOBALANOPS; ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

EID: 33747691082     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0289-y     Document Type: Article

References (28)

1. Bell SG, Harford-Cross CF, Wong LL (2001) Engineering the CYP101 system for in vivo oxidation of unnatural substrates. Protein Eng 14:797-802
2. Cherry JR, Fidantsef AL (2003) Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 14:438-443
3. Cruz A, Fernandes P, Cabral JMS, Pinheiro HM(2002) Effect of phase composition on the whole-cell bioconversion of β-sitosterol in biphase media. J Mol Catal B Enzym 19-20:371-375
4. Cruz A, Fernandes P, Cabral JMS, Pinheiro HM (2004) Solvent portioning and whole cell sitosterol bioconversion activity in aqueous-organic two-phase systems. Enzyme Microb Technol 34:342-353
5. Ernst M, Kaup B, Müller M, Bringer-Meyer S, Sahm H (2005) Enantioselective reduction of carbonyl compounds by whole-cell biotransformation, combining a formate dehydrogenase and a (R)-specific alcohol dehydrogenase. Appl Microbiol Biotechnol 66:629-634
6. Favre-Bulle O, Witholt B (1992) Biooxidation of n-octane by a recombinant Escherichia coli in a two-liquid-phase system: effect of medium components on cell growth and alkane oxidation activity. Enzyme Microb Technol 14:931-937
7. Gunsalus IC, Wagner GC (1978) Bacterial P-450cam methylene monooxygenase components: cytochrome m, putidaredoxin, and putidaredoxin reductase. Methods Enzymol 52:166-188
8. Ichinose H, Michizoe J, Maruyama T, Kamiya N, Goto M (2004) Electron-transfer reactions and functionalization of cytochrome P450cam monooxygenase system in reverse micelles. Langmuir 20:5564-5568
9. Joo H, Lin Z, Arnold FH (1999) Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation. Nature 399:670-673
10. Jose J, von Schwichow S (2004) Autodisplay of active sorbitol dehydrogenase (SDH) yields a whole cell biocatalyst for the synthesis of rare sugars. ChemBioChem 5:491-499
11. Kataoka M, Kita K, Wada M, Yasohara Y, Hasegawa J, Shimizu S (2003) Novel bioreduction system for the production of chiral alcohols. Appl Microbiol Biotechnol 62:437-445
12. Kim M, Rhee JS (1993) Lipid hydrolysis by Pseudomonasputida 3SK cultured in aqueous-organic two phase system. Enzyme Microb Technol 15:612-616
13. León R, Fernandes P, Pinheiro HM, Cabral JMS (1998) Whole-cell biocatalysis in organic media. Enzyme Microb Technol 23:483-500
14. Marconi AM, Kieboom J, de Bont JAM (1997) Improving the catabolic functions in the toluene-resistant strain Pseudomonas putidaS12. Biotechnol Lett 19:603-606
15. Na KS, Kuroda A, Takiguchi N, Ikeda T, Ohtake H, Kato J (2005) Isolation and characterization of benzene-tolerant Rhodococcus opecus strains. J Biosci Bioeng 99:378-382
16. Nelson DR, Kamataki T, Waxman DJ, Guengerich FP, Estabrook RW, Feyereisen R, Gonzalez FJ, Coon MJ, Gunsalus IC, Gotoh O, Okuda K, Nebert DW (1993) The P450 superfamily: update on new sequences, gene-mapping, accession numbers, early trivial names of enzymes, and nomenclature. DNA Cell Biol 12:1-51
17. Omura T, Sato E (1964) Carbon monoxide binding pigment of liver microsomes. J Biol Chem 239:2370-2378
18. Panke S, Held M, Wubbolts MG, Witholt B, Schmid A (2002) Pilot-scale production of (S)-styrene oxide from styrene by recombinant Escherichia coli synthesizing styrene monooxygenase. Biotechnol Bioeng 80:33-41
19. Poulos TL, Finzel BC, Howard AJ (1987) High resolution crystal structure of cytochrome P450cam. J Mol Biol 195:687-700
20. Sanno Y, Wilson TH, Lin ECC (1968) Control of permeation to glycerol in cell of Escherichia coli. Biochem Biophys Res Commun 32:344-349
21. Schmid A, Sonnleitner B, Witholt B (1998) Medium chain length alkane solvent-cell transfer rates in two-liquid phase, Pseudomonas oleovorans cultures. Biotechnol Bioeng 60:10-23
22. Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B (2001) Industrial biocatalysis today and tomorrow. Nature 409:258-268
23. Schneider S, Wubbolts MG, Oesterhelt G, Sanglard D, Witholt B (1999) Controlled regioselectivity of fatty acid oxidation by whole cell producing cytochrome P450BM-3 monooxygenase under varied dissolved oxygen concentrations. Biotechnol Bioeng 64:333-341
24. Sello G, Bernasconi S, Orsini F, Tansi M, Galli E, Gennaro PD, Bestetti G (2004) Organic phase effect in the biphasic bioconversion of substituted naphthalenes by engineered E. coli containing P. fluorescens N3 dioxygenase. J Mol Catal B Enzym 29:181-186
25. Simpson HD, Alphand V, Furstoss R (2001) Microbiological transformations 49. Asymmetric biocatalysed Baeyer-Villiger oxidation: improvement using a recombinant Escherichia coli whole cell biocatalyst in the presence of an adsorbent resin. J Mol Catal B Enzym 16:101-108
26. Wilms B, Wiese A, Syldatk C, Mattes R, Altenbuchner J (2001) Development of the Escherichia coli whole cell biocatalyst for the production of L-amino acids. J Biotechnol 86:19-30
27. Wubbolts MG, Hoven J, Melgert B, Witholt B (1994) Efficient production of optically active styrene epoxides in two-liquid phase cultures. Enzyme Microb Technol 16:887-894
28. Wubbolts MG, Favre-Bulle O, Witholt B (1996) Biosynthesis of synthons in two-liquid-phase media. Biotechnol Bioeng 52:301-308