Construction of a cytosolic firefly luciferase reporter cassette for use in PCR-mediated gene deletion and fusion in Saccharomyces cerevisiae

Yeast

Volumn 29, Issue 12, 2012, Pages 505-517

  Ainsworth, W B ^a   Rome, C M ^a   Hjortso M A ^a   Benton, M G ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

Author keywords

Bioluminescence; Photinus pyralis; Promoter; Reporter; Saccharomyces cerevisiae; YEGFP

Indexed keywords

FIREFLY LUCIFERASE;

ARTICLE; BIOLUMINESCENCE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CYTOSOL; EXPRESSION VECTOR; GENE; GENE CASSETTE; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; GENE FUSION; GENE INACTIVATION; HUG1 GENE; IN VIVO STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE;

ANIMALS; CYTOSOL; ENZYME INDUCTION; FIREFLIES; GENE DELETION; GENE FUSION; GENE KNOCKOUT TECHNIQUES; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUCIFERASES, FIREFLY; LUMINESCENT AGENTS; LUMINESCENT MEASUREMENTS; PEPTIDE ELONGATION FACTOR 1; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

PHOTINUS PYRALIS; SACCHAROMYCES CEREVISIAE;

EID: 84871018274     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.2931     Document Type: Article

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