SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH

Cell Death and Differentiation

Volumn 19, Issue 11, 2012, Pages 1815-1825

  Pellegrini, L ^a   Pucci, B ^b   Villanova, L ^a   Marino, M L ^c   Marfe, G ^d   Sansone, L ^a   Vernucci, E ^a   Bellizzi, D ^e   Reali, V ^a   Fini, M ^b   Russo, M A ^a,b   Tafani, M ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b IRCCS SAN RAFFAELE PISANA   (Italy)

^c ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^d UNIVERSITY OF ROME TOR VERGATA   (Italy)

^e UNIVERSITY OF CALABRIA   (Italy)

Author keywords

intracellular pH; mitochondria; ROS; SIRT3

Indexed keywords

CARBONATE DEHYDRATASE V; HEXOKINASE; HEXOKINASE ISOENZYME; PROTEIN BAX; REACTIVE OXYGEN METABOLITE; SIRTUIN 3; STAUROSPORINE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CATALYSIS; CELL PH; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYPOXIA; MAMMAL CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; OXIDATIVE STRESS; PRIORITY JOURNAL; REGULATORY MECHANISM;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CARBONIC ANHYDRASES; CELL HYPOXIA; CELL LINE, TUMOR; ENZYME INHIBITORS; HELA CELLS; HEXOKINASE; HUMANS; HYDROGEN-ION CONCENTRATION; K562 CELLS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; PROTEIN BINDING; REACTIVE OXYGEN SPECIES; RNA INTERFERENCE; RNA, SMALL INTERFERING; SIRTUIN 3; STAUROSPORINE;

MAMMALIA;

EID: 84870981253     PISSN: 13509047     EISSN: 14765403     Source Type: Journal    
DOI: 10.1038/cdd.2012.62     Document Type: Article

References (37)

1. Dutnall RN, Pillus L. Deciphering NAD-dependent deacetylases. Cell 2001; 105:161-164.
2. Guarente L, Kenyon C. Genetic pathways that regulate ageing in model organisms. Nature 2000; 408: 255-262.
3. Haigis MC, Guarente LP. Mammalian sirtuins-emerging roles in physiology, aging, and calorie restriction. Genes Dev 2006; 20: 2913-2921.
4. Michan S, Sinclair D. Sirtuins in mammals: insights into their biological function. Biochem J 2007; 404: 1-13.
5. Saunders LR, Verdin E. Sirtuins: critical regulators atthe crossroads between cancer and aging. Oncogene 2007; 26: 5489-5504.
6. Brachmann CB, Sherman JM, Devine SE, Cameron EE, Pillus L, Boeke JD. The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability. Genes Dev 1995; 9: 2888-2902.
7. Sauve AA, Celic I, Avalos J, Deng H, Boeke JD, Schramm VL. Chemistryof gene silencing: the mechanism of NAD+-dependent deacetylation reactions. Biochemistry 2001; 40: 15456-15463.
8. Imai S, Armstrong CM, Kaeberlein M, Guarente L. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 2000; 403: 795-800.
9. Michishita E, Park JY, Burneskis JM, Barrett JC, Horikawa I. Evolutionarilyconservedand nonconserved cellular localizations and functions of human SIRT proteins. Mol Biol Cell 2005; 16: 4623-4635.
10. Verdin E, Hirschey MD, Finley LW, Haigis MC. Sirtuin regulation of mitochondria: energy production, apoptosis, and signaling. Trends Biochem Sci 2010; 35: 669-675.
11. Lombard DB, Alt FW, Cheng HL, Bunkenborg J, Streeper RS, Mostoslavsky R, et al. Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation. Mol Cell Biol 2007; 27: 8807-8814.
12. Shulga N, Wilson-Smith R, Pastorino JG. Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria. J Cell Sci 2010; 123: 894-902.
13. Giorgio V, Soriano ME, Basso E, Bisetto E, Lippe G, Forte Ma, et al. Cyclophilin D in mitochondrial pathophysiology. Biochim Biophys Acta 2010; 1797:1113-1118.
14. Shulga N, Pastorino JG. Ethanol sensitizes mitochondria to the permeability transition by inhibiting deacetylation of cyclophilin-D mediated by sirtuin-3. J Cell Sci 2010; 123: 4117-4127.
15. Li Y, Johnson N, Capano M, Edwards M, Crompton M. Cyclophilin-D promotes the mitochondrial permeability transition but has opposite effects on apoptosis and necrosis. Biochem J 2004; 383:101-109.
16. Schubert A, Grimm S. Cyclophilin Dacomponentofthepermeabilitytransition- pore, isan apoptosis repressor. Cancer Res 2004; 64: 85-93.
17. Krysko DV, Vanden Berghe T, Parthoens E, D'Herde K, Vandenabeele P. Methods for distinguishing apoptotic from necrotic cells and measuring their clearance. Methods Enzymol 2008; 442: 307-341.
18. Susin SA, Zamzami N, Kroemer G. Mitochondria as regulators of apoptosis: doubt no more. Biochim Biophys Acta 1998; 1366:151-165.
19. Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Inductionof apoptoticprogram incell-free extracts: requirement for dATP and cytochrome c. Cell 1996; 86:147-157.
20. Nechushtan A, Smith CL, Hsu YT, Youle RJ. Conformation of the Bax C-terminus regulates subcellular location and cell death. EMBO J1999; 18: 330-341.
21. Tafani M, Cohn JA, Karpinich NO, Rothman RJ, Russo MA, Farber JL. Regulation of intracellular pH mediates Bax activation in HeLa cells treated with staurosporine or tumor necrosis factor-alpha. J Biol Chem 2002; 277: 49569-49576.
22. Mathupala SP, Ko YH, Pedersen PL. Hexokinase II cancer'sdouble-edged sword acting as both facilitator and gatekeeper of malignancy when bound to mitochondria. Oncogene 2006; 25: 4777-4786.
23. Qiu X, Brown K, Hirschey MD, Verdin E, Chen D. Calorie restriction reduces oxidative stress by SIRT3-mediated SOD2 activation. Cell Metab 2010; 12: 662-667.
24. Madshus IH. Regulation of intracellular pH in eukaryotic cells. Biochem J 1988; 250:1-8.
25. Gilmour KM. Perspectives on carbonic anhydrase. Comp Biochem Physiol A Mol Integr Physiol 2010; 157: 193-197.
26. Penninger JM, Kroemer G. Mitochondria, AIF and caspases-rivaling for cell death execution. Nat Cell Biol 2003; 5: 97-99.
27. Bellizzi D, Dato S, Cavalcante P, Covello G, Di Cianni F, Passarino G, et al. Characterization of a bidirectional promoter shared between two human genes related to aging: SIRT3 and PSMD13. Genomics 2007; 89:143-150.
28. Warburg O. On the origin of cancer cells. Science 1956; 123: 309-314.
29. Mathupala SP, Rempel A, Pedersen PL. Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. J Biol Chem 2001; 276: 43407-43412.
30. Vághy PL. Role of mitochondrial oxidative phosphorylation in the maintenance of intracellular pH. J Mol Cell Cardiol 1979; 11: 933-940.
31. Acin-Perez R, Salazar E, Kamenetsky M, Buck J, Levin LR, Manfredi G. Cyclic AMP produced inside mitochondria regulates oxidative phosphorylation. Cell Metab 2009; 9: 265-276.
32. Solaini G, Baracca A, Lenaz G, Sgarbi G. Hypoxia and mitochondrial oxidative metabolism. Biochim Biophys Acta 2010; 1797:1171-1177.
33. Cartron PF, Oliver L, Mayat E, Meflah K, Vallette FM. Impact of pH on Bax alpha conformation, oligomerisation and mitochondrial integration. FEBS Lett 2004; 578:41-46.
34. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998; 28:1309-1312.
35. Sundaresan NR, Samant SA, Pillai VB, Rajamohan SB, Gupta MP. SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70. Mol Cell Biol 2008; 28: 6384-6401.
36. Li A, Xue Y, Jin C, Wang M, Yao X. Prediction of Nepsilon-acetylation on internal lysines implemented in Bayesian Discriminant Method. Biochem Biophys Res Commun 2006; 1: 818-824.
37. Maren TH. A simplified micromethod for the determination of carbonic anhydrase and its inhibitors. JPharmacol Exp Ther 1960; 130: 26-29.