Preparation and characterization of mucus-penetrating papain/poly(acrylic acid) nanoparticles for oral drug delivery applications

Journal of Nanoparticle Research

Volumn 15, Issue 1, 2013, Pages

  Müller, Christiane ^a   Leithner, Katharina ^a   Hauptstein, Sabine ^a   Hintzen, Fabian ^a   Salvenmoser, Willi ^a   Bernkop Schnürch, Andreas ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Mucus barrier; Mucus penetrating particles; Oral drug delivery; Papain; Poly(acrylic acid)

Indexed keywords

ADHESIVES; CARBOXYLIC ACIDS; CONTROLLED DRUG DELIVERY; DIFFUSION; ENDOTHELIAL CELLS; NANOPARTICLES; PAPAIN; PARTICLE SIZE; PARTICLE SIZE ANALYSIS; POLYETHYLENES; ZETA POTENTIAL;

FUNCTIONALIZED NANOPARTICLES; MACROMOLECULAR COMPOUNDS; MUCUS BARRIER; MUCUS-PENETRATING PARTICLES; NEGATIVE ZETA POTENTIALS; ORAL DRUG DELIVERY; PHARMACEUTICAL FIELDS; POLY(ACRYLIC ACID );

TARGETED DRUG DELIVERY;

NANOPARTICLE; PAPAIN; POLYACRYLIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIFFUSION CHAMBER; DRUG DELIVERY SYSTEM; HYDROPHILICITY; INTESTINE; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN DEGRADATION; VISCOSITY; ZETA POTENTIAL;

EID: 84870826859     PISSN: 13880764     EISSN: 1572896X     Source Type: Journal    
DOI: 10.1007/s11051-012-1353-z     Document Type: Article

References (36)

1. Bernkop-Schnürch A (2000) Chitosan and its derivatives: potential excipients for peroral peptide delivery systems. Int J Pharm 194(1):1-13. doi: 10.1016/s0378-5173(99)00365-8
2. Bernkop-Schnürch A, Weithaler A, Albrecht K, Greimel A (2006) Thiomers: preparation and in vitro evaluation of a mucoadhesive nanoparticulate drug delivery system. Int J Pharm 317(1):76-81. doi: 10.1016/j.ijpharm.2006.02. 044 (Pubitemid 43866341)
3. Bradford MM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 72(1-2):248-254. doi: 10.1006/abio.1976.9999
4. Carlsson N, Borde A, Wolfel S, Akerman B, Larsson A (2011) Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers. Anal Biochem 411(1):116-121. doi: 10.1016/j.ab.2010.12. 026
5. Cone RA (2009) Barrier properties of mucus. Adv Drug Deliv Rev 61(2):75-85. doi: 10.1016/j.addr.2008.09.008
6. Crater JS, Carrier RL (2010) Barrier properties of gastrointestinal mucus to nanoparticle transport. Macromol Biosci 10(12):1473-1483. doi: 10.1002/mabi.201000137
7. Cu Y, Saltzman WM (2009) Controlled surface modification with poly(ethylene)glycol enhances diffusion of PLGA nanoparticles in human cervical mucus. Mol Pharm 6(1):173-181. doi: 10.1021/mp8001254
8. da Silva CR, Oliveira MBN, Motta ES, de Almeida GS, Varanda LL, de Padula M et al (2010) Genotoxic and cytotoxic safety evaluation of papain (Carica papaya L.) using in vitro assays. J Biomed Biotechnol. doi: 10.1155/2010/197898
9. Dautzenberg H, Hartmann J, Grunewald S, Brand F (1996) Stoichiometry and structure of polyelectrolyte complex particles in diluted solutions, Berichte Der Bunsen-Gesellschaft-Physical Chemistry. Chem Phys 100:1024-1032 (Pubitemid 126752747)
10. Dawson M, Krauland E, Wirtz D, Hanes J (2004) Transport of polymeric nanoparticle gene carriers in gastric mucus. Biotechnol Prog 20:851-857 (Pubitemid 38784024)
11. Dünnhaupt S, Barthelmes J, Hombach J, Sakloetsakun D, Arkhipova V, Bernkop- Schnürch A (2011) Distribution of thiolated mucoadhesive nanoparticles on intestinal mucosa. Int J Pharm 408:191-199
12. Emerich DF, Thanos CG (2007) Targeted nanoparticle-based drug delivery and diagnosis. J Drug Target 15(3):163-183. doi: 10.1080/10611860701231810 (Pubitemid 46562629)
13. Gauthier MA, Klok HA (2010) Polymer-protein conjugates: an enzymatic activity perspective. Polym Chem 1(9):1352-1373. doi: 10.1039/c0py90001j
14. Grabovac V, Guggi D, Bernkop-Schnurch A (2005) Comparison of the mucoadhesive properties of various polymers. Adv Drug Deliv Rev 57(11):1713-1723. doi: 10.1016/j.addr.2005.07.006 (Pubitemid 41443475)
15. Hoyer H, Schlocker W, Krum K, Bernkop-Schnürch A (2008) Preparation and evaluation of microparticles from thiolated, polymers via air jet milling. Eur J Pharm Biopharm 69:476-485
16. Itoyama K, Tanibe H, Hayashi T, Ikada Y (1994) Spacer effects on enzymatic-activity of papain immobilized onto porous chitosan beads. Biomaterials 15(2):107-112. doi: 10.1016/0142-9612(94)90258-5 (Pubitemid 24060608)
17. Izumi T, Hirata M, Takahashi K, Kokufuta E (1994) Complexation of papain with strong polyanions and enzymatic-activities of the resulting complexes. J Macromol Sci A31(1):39-51. doi: 10.1080/10601329409349716
18. Kilara A, Shahani KM, Wagner FW (1977) Preparation and properties of immobilized papain and lipase. Biotechnol Bioeng 19(11):1703-1714. doi: 10.1002/bit.260191109 (Pubitemid 8218320)
19. Lai SK, Wang YY, Hanes J (2009) Mucus-penetrating nanoparticles for drug and gene delivery to mucosal tissues. Adv Drug Deliv Rev 61:158-171
20. Li G, Raman VK, Xie WC, Gross RA (2008) Protease-catalyzed co-oligomerizations of l-leucine ethyl ester with l-glutamic acid diethyl ester: sequence and chain length distributions. Macromolecules 41(19):7003-7012. doi: 10.1021/ma800946d
21. Majima Y, Inagaki M, Hirata K, Takeuchi K, Morishita A, Sakakura Y (1988) The effect of an orally-administered proteolytic-enzyme on the elasticity and viscosity of nasal mucus. Arch OtoRhinoLaryngol 244(6):355-359. doi: 10.1007/bf00497464 (Pubitemid 18054465)
22. Marschütz MK, Bernkop-Schnürch A (2002) Thiolated polymers: self-crosslinking properties of thiolated 450 kDa poly(acrylic acid) and their influence on mucoadhesion. Eur J Pharm Sci 15(4):387-394. doi: 10.1016/s0928-0987(02)00025-8 (Pubitemid 34436926)
23. Mitchel REJ, Chaiken IM, Smith EL (1970) Complete amino acid sequence of papain - additions and corrections. J Biol Chem 245(14):3485
24. Nordman H, Davies JR, Herrmann A, Karlsson NG, Hansson GC, Carlstedt I (1997) Mucus glycoproteins from pig gastric mucose: identification of different mucin populations from the surface epithelium. Biochem J 326:903-910 (Pubitemid 27438068)
25. Norris DA, Sinko PJ (1997) Effect of size, surface charge, and hydrophobicity on the translocation of polystyrene microspheres through gastrointestinal mucin. J Appl Polym Sci 63(11):1481-1492. doi: 10.1002/(sici)1097-4628(19970314)63:11 (Pubitemid 127598331)
26. Olmsted SS, Padgett JL, Yudin AI, Whaley KJ, Moench TR, Cone RA (2001) Diffusion of macromolecules and virus-like particles in human cervical mucus. Biophys J 81(4):1930-1937 (Pubitemid 32917145)
27. Peppas NA, Hansen PJ, Buri PA (1984) A theory of molecular-diffusion in the intestinal mucus. Int J Pharm 20(1-2):107-118. doi: 10.1016/0378-5173(84) 90222-9
28. Rosenthal M, Traut HF (1951) The mucolytic action of papain for cell concentration in the diagnosis of gastric cancer. Cancer 4(1):147-149. doi: 10.1002/1097-0142(195101)4:1
29. Sangeetha K, Abraham TE (2006) Chemical modification of papain for use in alkaline medium. J Mol Catal B 38(3-6):171-177. doi: 10.1016/j.molcatb.2006.01. 003
30. Schlamowitz M, Peterson LU (1959) Studies on the optimum pH for the action of pepsin on native and denaturated bovine serum albumin and bovine hemoglobin. J Biol Chem 234(12):3137-3145
31. Shu SJ, Sun L, Zhang XG, Wu ZM, Wang Z, Li CX (2011) Polysaccharides- based polyelectrolyte nanoparticles as protein drugs delivery system. J Nanopart Res 13:3657-3670
32. Sipos T, Merkel JR (1970) An effect of calcium ions on activity, heat stability, and structure of trypsin. Biochemistry 9 (14):2766-2775. doi: 10.1021/bi00816a003
33. Soppimath KS, Aminabhavi TM, Kulkarni AR, Rudzinski WE (2001) Biodegradable polymeric nanoparticles as drug delivery devices. J Control Release 70(1-2):1-20. doi: 10.1016/s0168-3659(00)00339-4 (Pubitemid 32144788)
34. Spackman DH, Stein WH, Moore S (1960) Disulfide bonds of ribonuclease. J Biol Chem 235(3):648-659
35. Tang BC, Dawson M, Lai SK, Wang YY, Suk JS, Yang M, Zeitlin P, Boyle MP, Fu J, Hanes J (2009) Biodegradable polymer nanoparticles that rapidly penetrate the human mucus barrier. Proc Natl Acad Sci USA 106(46):19268-19273. doi: 10.1073/pnas.0905998106
36. Thaurer MH, Deutel B, Schlocker W, Bernkop-Schnürch A (2009) Development of nanoparticulate drug delivery systems based on thiolated poly(acrylic acid). J Microencapsul 26:187-194