Genotoxic and cytotoxic safety evaluation of papain (Carica papaya L.) using in vitro assays

Journal of Biomedicine and Biotechnology

Volumn 2010, Issue , 2010, Pages

  Silva, Claudia R Da ^a,b,c   Oliveira, Marcia B N ^a   Motta, Ellen S ^a   Almeida, Gabriella S De ^a,b   Varanda, Leandro L ^a,b   Pádula, Marcelo De ^b,c   Leito, Alvaro C ^b   Caldeira De Arajo, Adriano ^a  

^a Department of Physiological Sciences   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c Faculdade de Farmacia   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEN PEROXIDE; PAPAIN; PLASMID DNA;

AGAR GEL ELECTROPHORESIS; ANTIOXIDANT ACTIVITY; ARTICLE; BACTERIAL STRAIN; CELL PROTECTION; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG SAFETY; ESCHERICHIA COLI; GENOTOXICITY; GROWTH INHIBITION; IN VITRO STUDY; MUTAGENIC ACTIVITY; NONHUMAN; OXIDATIVE STRESS; REVERTANT;

CARICA PAPAYA; ESCHERICHIA COLI;

EID: 77953492064     PISSN: 11107243     EISSN: 11107251     Source Type: Journal    
DOI: 10.1155/2010/197898     Document Type: Article

References (42)

1. Valerio L. G. Jr., Gonzales G. F., Toxicological aspects of the South American herbs cat's claw (Uncaria tomentosa) and maca (Lepidium meyenii): a critical synopsis Toxicological Reviews 2005 24 1 11 35
2. Mengs U., Toxic effects of sennosides in laboratory animals and in vitro Pharmacology 1988 36 1 180 187
3. Leito A. C., Braga R. S., Mutagenic and genotoxic effects of mate (Ilex paraguariensis) in prokaryotic organisms Brazilian Journal of Medical and Biological Research 1994 27 7 1517 1525
4. Fonseca C. A. S., Otto S. S., Paumgartten F. J. R., Leito A. C., Nontoxic, mutagenic, and clastogenic activities of mate-chimarrao (Ilex paraguariensis) Journal of Environmental Pathology, Toxicology and Oncology 2000 19 4 333 346
5. Matthews S. C., Camacho A., Lawson K., Dimsdale J. E., Use of herbal medications among 200 psychiatric outpatients: prevalence, patterns of use, and potential dangers General Hospital Psychiatry 2003 25 1 24 26
6. Ferreira-Machado S. C., Rodrigues M. P., Nunes A. P. M., Genotoxic potentiality of aqueous extract prepared from Chrysobalanus icaco L. leaves Toxicology Letters 2004 151 3 481 487
7. Dciga-Campos M., Rivero-Cruz I., Arriaga-Alba M., Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine The Journal of Ethnopharmacology 2007 110 2 334 342
8. Silva C. R., Monteiro M. R., Rocha H. M., Assessment of antimutagenic and genotoxic potential of senna (Cassia angustifolia Vahl.) aqueous extract using in vitro assays Toxicology in Vitro 2008 22 1 212 218
9. Teschke R., Genthner A., Wolff A., Kava hepatotoxicity: comparison of aqueous, ethanolic, acetonic kava extracts and kava-herbs mixtures Journal of Ethnopharmacology 2009 123 3 378 384
10. Starley I. F., Mohammed P., Schneider G., Bickler S. W., The treatment of paediatric burns using topical papaya Burns 1999 25 7 636 639
11. Oloyede O. I., Chemical profile of unripe pulp of Carica papaya Pakistan Journal of Nutrition 2005 4 6 379 381
12. El Moussaoui A., Nijs M., Paul C., Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism Cellular and Molecular Life Sciences 2001 58 4 556 570
13. Webman E. J., Edlin G., Mower H. F., Free radical scavenging activity of papaya juice International Journal of Radiation Biology 1989 55 3 347 351
14. Mehdipour S., Yasa N., Dehghan G., Antioxidant potentials of Iranian Carica papaya juice in vitro and in vivo are comparable to -tocopherol Phytotherapy Research 2006 20 7 591 594
15. Hewitt H., Whittle S., Lopez S., Bailey E., Weaver S., Topical use of papaya in chronic skin ulcer therapy in Jamaica West Indian Medical Journal 2000 49 1 32 33
16. Almeida C. E., Nascimento C. C., Brando E. S., Manual para Realizao de Curativos 2002 Rio de Janeiro, Brasil Cultura Mdica
17. Maron D. M., Ames B. N., Revised methods for the Salmonella mutagenicity test Mutation Research 1983 113 3-4 173 215
18. Blanco M., Urios A., Martinez A., New Escherichia coli WP2 tester strains highly sensitive to reversion by oxidative mutagens Mutation Research 1998 413 2 95 101
19. Rosenkranz H. S., A paradigm for determining the relevance of short-term assays: application to oxidative mutagenesis Mutation Research 2002 508 1-2 21 27
20. Horn R. C., Ferro Vargas V. M., Antimutagenic activity of extracts of natural substances in the salmonella/microsome assay Mutagenesis 2003 18 2 113 118
21. Imlay J. A., Linn S., DNA damage and oxygen radical toxicity Science 1988 240 4857 1302 1309
22. Imlay J. A., Pathways of oxidative damage Annual Review of Microbiology 2003 57 395 418
23. Mates J. M., Perez-Gomez C., Nunez de Castro I. N., Antioxidant enzymes and human diseases Clinical Biochemistry 1999 32 8 595 603
24. Bjelland S., Seeberg E., Mutagenicity, toxicity and repair of DNA base damage induced by oxidation Mutation Research 2003 531 1-2 37 80
25. Cooke M. S., Evans M. D., Dizdaroglu M., Lunec J., Oxidative DNA damage: mechanisms, mutation, and disease Journal of the Federation of American Societies for Experimental Biology 2003 17 10 1195 1214 (Pubitemid 36775767)
26. Asad N. R., Asad L. M. B. O., de Almeida C. E. B., Felzenszwalb I., Cabral-Neto J. B., Leito A. C., Several pathways of hydrogen peroxide action that damage the E. coli genome Genetics and Molecular Biology 2004 27 2 291 303
27. Emerit J., Edeas M., Bricaire F., Neurodegenerative diseases and oxidative stress Biomedicine Pharmacotherapy 2004 58 1 39 46
28. Shi Q., Gibson G. E., Oxidative stress and transcriptional regulation in Alzheimer disease Alzheimer Disease and Associated Disorders 2007 21 4 276 291
29. Chanwitheesuk A., Teerawutgulrag A., Rakariyatham N., Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand Food Chemistry 2005 92 3 491 497
30. Howard-Flanders P., Theriot L., Mutants of Escherichia coli K12 defective in DNA repair and genetic recombination Genetics 1966 53 1137 1150
31. Demple B., Halbrook J., Linn S., Escherichia coli xth mutants are hypersensitive to hydrogen peroxide Journal of Bacteriology 1983 153 2 1079 1082
32. Hanahan D., Studies on transformation of Escherichia coli with plasmids Journal of Molecular Biology 1983 166 4 557 580
33. Quillardet P., Hofnung M., The SOS Chromotest, a colorimetric bacterial assay for genotoxins: procedures Mutation Research 1985 147 3 65 78
34. Boiteux S., Huisman O., Isolation of a formamidopyrimidine-DNA glycosylase (fpg) mutant of Escherichia coli K12 Molecular General Genetics 1989 215 2 300 305
35. Sambrook J., Fritisch E. F., Maniatis T., Extraction and purification of plasmid DNA Molecular Cloning: A Laboratory Manual 1989 2nd book 3 New York, NY, USA Cold Spring Harbor Laboratory
36. Alfaro C., Urios A., Gonzalez M. C., Moya P., Blanco M., Screening for metabolites from Penicillium novae-zeelandiae displaying radical-scavenging activity and oxidative mutagenicity: isolation of gentisyl alcohol Mutation Research 2003 539 1-2 187 194
37. Friedberg E., Walker G. C., Siede W., Wood R. D., Schultz R. A., Ellenberger T., DNA Repair and Mutagenesis 2006 Washington, DC, USA American Society for Microbiology
38. Mortelmans K., Riccio E. S., The bacterial tryptophan reverse mutation assay with Escherichia coli WP2 Mutation Research 2000 455 1-2 61 69
39. Schulz I., Mahler H.-C., Boiteux S., Epe B., Oxidative DNA base damage induced by singlet oxygen and photosensitization: recognition by repair endonucleases and mutagenicity Mutation Research 2000 461 2 145 156
40. Wallace S. S., Biological consequences of free radical-damaged DNA bases Free Radical Biology and Medicine 2002 33 1 1 14
41. Reiniger I. W., Ribeiro da Silva C., Felzenszwalb I., Boldine action against the stannous chloride effect Journal of Ethnopharmacology 1999 68 13 345 348
42. Paes-Leme A. A., Motta E. S., de Mattos J. C. P., Dantas F. J.S., Bezerra R. J. A. C., Caldeira-de-Araujo A., Assessment of Aloe vera (L.) genotoxic potential on Escherichia coli and plasmid DNA Journal of Ethnopharmacology 2005 102 2 197 201