Interleukin-6 and lipopolysaccharide modulate hepcidin mRNA expression by Hepg2 cells

Biological Trace Element Research

Volumn 150, Issue 1-3, 2012, Pages 496-501

  Villarroel, Pia ^a   Le Blanc, Solange ^a   Arredondo, Miguel ^a  

^a UNIVERSITY OF CHILE   (Chile)

Author keywords

Hepcidin; HepG2 cells; Interleukin 6; Lipopolysaccharide

Indexed keywords

HEPCIDIN; INTERLEUKIN 6; IRON; LIPOPOLYSACCHARIDE; MESSENGER RNA; NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 2; TRANSFERRIN RECEPTOR;

ARTICLE; CELL LEVEL; CELL STRAIN HEPG2; CONCENTRATION RESPONSE; CONTROLLED STUDY; DMT1 GENE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HPC GENE; HUMAN; HUMAN CELL; INFLAMMATION; IRON METABOLISM; IRON STORAGE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ABSORPTION; ANTIMICROBIAL CATIONIC PEPTIDES; BIOLOGICAL TRANSPORT; CHLORIDES; DIETARY SUPPLEMENTS; FERRIC COMPOUNDS; GENE EXPRESSION REGULATION; HEP G2 CELLS; HEPATOCYTES; HUMANS; INTERLEUKIN-6; IRON; IRON OVERLOAD; IRON RADIOISOTOPES; LIPOPOLYSACCHARIDES; NITRILOTRIACETIC ACID; OSMOLAR CONCENTRATION; RECEPTORS, TRANSFERRIN; RNA, MESSENGER; SPECTROPHOTOMETRY, ATOMIC; TRANSCRIPTION FACTORS;

EID: 84870714390     PISSN: 01634984     EISSN: 15590720     Source Type: Journal    
DOI: 10.1007/s12011-012-9522-6     Document Type: Article

References (32)

1. Ganz T (2003) Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood 102:783-788
2. Park CH, Valore EV, Waring AJ, Ganz T (2001) Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem 276:7806-7810
3. Krause A, Neitz S, Mägert HJ, Schulz A, Forssmann WG, Schulz-Knappe P, Adermann K (2000) LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS 480:147-150
4. Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P, Loréal O (2001) A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, in overexpressed during iron overload. J Biol Chem 276:7811-7819
5. Donovan A, Lima CA, Pinkus JL (2005) The iron exporter ferroportin/Slc40a1 is essential for iron homeostasis. Cell Metabolism 1:191-200
6. Vyoral D, Petrák J (2005) Hepcidin: a direct link between iron metabolism and immunity. Int J Biochem Cell Biol 37:1768-1773
7. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J (2004) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306:2090-2093
8. Nemeth E (2010) Targeting the hepcidin-ferroportin axis in the diagnosis and treatment of anemias. Adv Hematol: Article ID 750643, 9 pages
9. Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S (2006) Suppression of hepcidin during anemia requires erythropoietic activity. Blood 108:3730-3735
10. Nemeth E, Ganz T (2006) Regulation of iron metabolism by hepcidin. Annu Rev Nutr 26:323-342
11. Theurl I, Theurl M, Seifert M (2008) Autocrine formation of hepcidin induces iron retention in human monocytes. Blood 111:2392-2399
12. Collins J, Wessling-Resnick M, Knutson M (2008) Hepcidin regulation of iron transport. J Nutr 138:22842-288
13. Papanikolaou G, Samuels ME, Ludwig EH, MacDonald ML, Franchini PL, Dubé MP, Andres L, MacFarlane J, Sakellaropoulos N, Politou M, Nemeth E, Thompson J, Risler JK, Zaborowska C, Babakaiff R, Radomski CC, Pape TD, Davidas O, Christakis J, Brissot P, Lockitch G, Ganz T, Hayden MR, Goldberg YP (2004) Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis. Nat Genet 36:77-82
14. Niederkofler V, Salie R, Arber S (2005) Hemojuvelin is essential for dietary iron sensing, and its mutation leads to severe iron overload. J Clin Invest 115:2180-2186
15. Nemeth E, Roetto A, Garozzo G, Ganz T, Camaschella C (2005) Hepcidin is decreased in TFR2 hemochromatosis. Blood 105:1803-1806
16. Nemeth E, Rivera S, Gabayan V, Keller C, Taudorf S, Pedersen BK, Ganz T (2004) IL6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. J Clin Invest 113:1271-1276
17. Kemna E, Pickkers P, Nemeth E, van der Hoeven H, Swinkels D (2005) Time-course analysis of hepcidin, serum iron, and plasma cytokine levels in humans injected with LPS. Blood 106:1864-1866
18. Anderson GJ, Frazer DM, Wilkins SJ, Becker EM, Millard KN, Murphy TL, McKie AT, Vulpe CD (2002) Relationship between intestinal iron-transporter expression, hepatic hepcidin levels and the control of iron absorption. Biochem Soc Trans 30:724-726
19. Fein E, Merle U, Ehehalt R, Herrmann T, Kulaksiz H (2007) Regulation of hepcidin in HepG2 and RINm5F cells. Peptides 28:951-957
20. Nicolas G, Bennoun M, Devaux I, Beaumont C, Grandchamp B, Kahn A, Vaulont S (2001) Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc Natl Acad Sci 98:8780-8785
21. von Bonsdorff L, Lindeberg E, Sahlstedt L, Lehto J, Parkkinen J (2002) Bleomycin-detectable iron assay for non-transferrin-bound iron in hematologic malignancies. Clin Chem 48:307-314
22. Mena NP, Esparza A, Tapia V, Valdés P, Núñez MT (2008) Hepcidin inhibits apical iron uptake in intestinal cells. Am J Physiol Gastrointest Liver Physiol 294:G192-G198
23. Grube BJ, Cochane CG, Ye RD, Green CE, McPhail ME, Ulevitch RJ, Tobias PS (1994) Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells. J Biol Chem 269:8477-8482
24. Nemeth E, Valore EV, Territo M, Schiller G, Lichtenstein A, Ganz T (2003) Hepcidin, a putative mediator of anemia of inflammation, is a type II acute phase protein. Blood 101:2461-2463
25. Du F, Qian C, Qian ZM, Wu XM, Xie H, Yung WH, Ke Y (2011) Hepcidin directly inhibits transferrin receptor 1 expression in astrocytes via a cyclic AMP-protein kinase a pathway. Glia 59:936-945
26. Dzikaite V, Holmström P, Stål P, Eckes K, Hagen K, Eggertsen G, Gåfvels M, Melefors O, Hultcrantz R (2006) Regulatory effects of tumor necrosis factor-alpha and interleukin-6 on HAMP expression in iron loaded rat hepatocytes. J Hepatol 44:544-519
27. Laftah AH, Sharma N, Brookes MJ, McKie AT, Simpson RJ, Iqbal TH, Tselepis C (2002) Tumour necrosis factor alpha causes hypoferraemia and reduced intestinal iron absorption in mice. Biochem J 397:61-67
28. Hunter HN, Fulton DB, Ganz T, Vogel HJ (2002) The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis. J Biol Chem 277:37597-37603
29. Meynard D, Vaja V, Sun CC, Corradini E, Chen S, López-Otín C, Grgurevic L, Hong CC, Stirnberg M, Gütschow M, Vukicevic S, Babitt JL, Lin HY (2011) Regulation of TMPRSS6 by BMP6 and iron in human cells and mice. Blood 118:747-756
30. Nai A, Pagani A, Silvestri L, Campostrini N, Corbella M, Girelli D, Traglia M, Toniolo D, Camaschella C (2011) TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals. Blood 118:4459-4462
31. Pagani A, Nai A, Corna G, Bosurgi L, Rovere-Querini P, Camaschella C, Silvestri L (2011) Low hepcidin accounts for the proinflammatory status associated with iron deficiency. Blood 118:736-746
32. Zhang AS, Anderson SA, Wang J, Yang F, DeMaster K, Ahmed R, Nizzi CP, Eisenstein RS, Tsukamoto H, Enns CA (2011) Suppression of hepatic hepcidin expression in response to acute iron deprivation is associated with an increase of matriptase-2 protein. Blood 117:1687-1699