Conjugating phosphospermines to siRNAs for improved stability in serum, intracellular delivery and RNAi-mediated gene silencing

Molecular Pharmaceutics

Volumn 9, Issue 12, 2012, Pages 3464-3475

  Paris, Clément ^a   Moreau, Valérie ^a   Deglane, Gaëlle ^a   Karim, Loukmane ^a   Couturier, Bernard ^a   Bonnet, Marie Elise ^a   Kedinger, Valérie ^a   Messmer, Mélanie ^a   Bolcato Bellemin, Anne Laure ^a   Behr, Jean Paul ^a   Erbacher, Patrick ^a   Lenne Samuel, Nathalie ^a  

^a Polyplus transfection   (France)

Author keywords

oligonucleotide conjugate; siRNA delivery; spermine; ZNA

Indexed keywords

CATION; MESSENGER RNA; NUCLEASE; OLIGOPHOSPHOSPERMINE; OLIGORIBONUCLEOTIDE; SMALL INTERFERING RNA; SPERMINE DERIVATIVE; SURVIVIN; UNCLASSIFIED DRUG;

ANION EXCHANGE; ARTICLE; BIOLOGICAL ACTIVITY; CELL MEMBRANE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME DEGRADATION; GENE DELIVERY SYSTEM; GENE SILENCING; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RNA INTERFERENCE; STATIC ELECTRICITY;

ANIMALS; BLOTTING, WESTERN; DRUG DELIVERY SYSTEMS; FLOW CYTOMETRY; GENE SILENCING; HELA CELLS; HUMANS; INHIBITOR OF APOPTOSIS PROTEINS; LUCIFERASES; MELANOMA, EXPERIMENTAL; MICE; PHOSPHORYLATION; REAL-TIME POLYMERASE CHAIN REACTION; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPERMINE; TUMOR CELLS, CULTURED;

EID: 84870559432     PISSN: 15438384     EISSN: 15438392     Source Type: Journal    
DOI: 10.1021/mp300278b     Document Type: Article

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