Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing

Transgenic Research

Volumn 21, Issue 6, 2012, Pages 1233-1243

  Ruiz López, Noemi ^a   Haslam, Richard P ^a   Venegas Calerón, Mónica ^a,c   Li, Tianbi ^a   Bauer, Joerg ^b,d   Napier, Johnathan A ^a   Sayanova, Olga ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b BASF SE   (Germany)

^c INSTITUTO DE LA GRASA   (Spain)

^d Metanomics GmbH   (Germany)

Author keywords

Arabidopsis; Desaturase; Elongase; Omega 3; Polyunsaturated fatty acids; Transgenic plants

Indexed keywords

ACYL COENZYME A; ACYL COENZYME A DESATURASE; ARACHIDONIC ACID; ICOSAPENTAENOIC ACID; OMEGA 3 FATTY ACID; PHOSPHOLIPID DESATURASE; UNSATURATED FATTY ACID;

ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; CROSS BREEDING; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLIC ENGINEERING; METABOLISM; TRANSGENIC PLANT;

ACYL COENZYME A; ARABIDOPSIS; ARACHIDONIC ACID; CROSSES, GENETIC; EICOSAPENTAENOIC ACID; FATTY ACID DESATURASES; FATTY ACIDS, OMEGA-3; FATTY ACIDS, UNSATURATED; METABOLIC ENGINEERING; PLANTS, GENETICALLY MODIFIED;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 84870366993     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-012-9596-0     Document Type: Article

References (35)

1. Abbadi A, Domergue F, Bauer J, Napier JA, Welti R, Zähringer U, Cirpus P, Heinz E (2004) Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16: 2734-2748.
2. Bates PD, Browse J (2011) The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds. Plant J. doi: 10. 1111/j. 1365-313X. 2011. 04693. x.
3. Bates PD, Ohlrogge JB, Pollard M (2007) Incorporation of newly synthesized fatty acids into cytosolic glycerolipids in pea leaves occurs via acyl editing. J Biol Chem 282: 31206-31216.
4. Cahoon EB, Dietrich CR, Meyer K, Damude HG, Dyer JM, Kinney AJ (2006) Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds. Phytochemistry 67: 1166-1176.
5. Cheng B, Wu G, Vrinten P, Falk K, Bauer J, Qiu X (2010) Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters. Transgenic Res 19: 221-229.
6. Cressey D (2009) Aquaculture: future fish. Nature 458: 398-400.
7. Damude HG, Zhang H, Farrall L, Ripp KG, Tomb JF, Hollerbach D, Yadav NS (2006) Identification of bifunctional Δ12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants. Proc Natl Acad Sci USA 103: 9446-9451.
8. Domergue F, Abbadi A, Ott C, Zank TK, Zähringer U, Heinz E (2003) Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. J Biol Chem 278: 35115-35126.
9. Domergue F, Abbadi A, Heinz E (2005a) Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trends Plant Sci 10: 112-116.
10. Domergue F, Abbadi A, Zähringer U, Moreau H, Heinz E (2005b) In vivo characterization of the first acyl-CoA Δ6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem J 389: 483-490.
11. Dyer JM, Stymne S, Green AG, Carlsson AS (2008) High-value oils from plants. Plant J 54: 640-655.
12. Garcia SM, Rosenberg AA (2010) Food security and marine capture fisheries: characteristics, trends, drivers and future perspectives. Philos Trans R Soc Lond B Biol Sci 365: 2869-2880.
13. Graham IA, Larson T, Napier JA (2007) Rational metabolic engineering of transgenic plants for biosynthesis of omega-3 polyunsaturates. Curr Opin Biotechnol 18: 142-147.
14. Hoffmann M, Wagner M, Abbadi A, Fulda M, Feussner I (2008) Metabolic engineering of ω3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J Biol Chem 283: 22352-22362.
15. Hong H, Datla N, Reed DW, Covello PS, MacKenzie SL, Qiu X (2002) High-level production of gamma-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare. Plant Physiol 129: 354-362.
16. Kajikawa M, Yamato KT, Kohzu Y, Nojiri M, Sakuradani E, Shimizu S, Sakai Y, Fukuzawa H, Ohyama K (2004) Isolation and characterization of Δ6-desaturase, an ELO-like enzyme and Δ5-desaturase from the liverwort Marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Plant Mol Biol 54: 335-352.
17. Larson TR, Graham IA (2001) A novel technique for the sensitive quantification of acyl CoA esters from plant tissues. Plant J 25: 115-125.
18. Meyer A, Kirsch H, Domergue F, Abbadi A, Sperling P, Bauer J, Cirpus P, Zank TK, Moreau H, Roscoe TJ, Zähringer U, Heinz E (2004) Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J Lipid Res 45: 1899-1909.
19. Napier JA (2007) The production of unusual fatty acids in transgenic plants. Annu Rev Plant Biol 58: 295-319.
20. Napier JA, Graham IA (2010) Tailoring plant lipid composition: designer oilseeds come of age. Curr Opin Plant Biol 13: 330-337.
21. Napier JA, Sayanova O, Qi B, Lazarus CM (2004) Progress toward the production of long-chain polyunsaturated fatty acids in transgenic plants. Lipids 39: 1067-1075.
22. Petrie JR, Shrestha P, Mansour MP, Nichols PD, Liu Q, Singh SP (2010) Metabolic engineering of omega-3 long-chain polyunsaturated fatty acids in plants using an acyl-CoA Δ6-desaturase with omega3-preference from the marine microalga Micromonas pusilla. Metab Eng 12: 233-240.
23. Qi B, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier JA, Stobart AK, Lazarus CM (2004) Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol 22: 739-745.
24. Qiu X, Hong H, MacKenzie SL (2001) Identification of a Δ4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea. J Biol Chem 276: 31561-31566.
25. Robert SS (2006) Production of eicosapentaenoic and docosahexaenoic acid-containing oils in transgenic land plants for human and aquaculture nutrition. Mar Biotechnol (NY) 8: 103-109.
26. Saravanan P, Davidson NC, Schmidt EB, Calder PC (2010) Cardiovascular effects of marine omega-3 fatty acids. Lancet 376: 540-550.
27. Sayanova OV, Napier JA (2004) Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry 65: 147-158.
28. 5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco. Proc Natl Acad Sci USA 94: 4211-4216.
29. Sayanova O, Haslam R, Guschina I, Lloyd D, Christie WW, Harwood JL, Napier JA (2006a) A bifunctional Δ12, Δ15-desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids. J Biol Chem 281: 36533-36541.
30. Sayanova O, Haslam R, Venegas-Calerón M, Napier JA (2006b) Identification of Primula "front-end" desaturases with distinct n-6 or n-3 substrate preferences. Planta 224: 1269-1277.
31. Sayanova O, Ruiz-Lopez N, Haslam RP, Napier JA (2012) The role of Δ6-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants. Plant Biotechnol J 10: 195-206.
32. Venegas-Calerón M, Sayanova O, Napier JA (2010) An alternative to fish oils: metabolic engineering of oil-seed crops to produce omega-3 long chain polyunsaturated fatty acids. Prog Lipid Res 49: 108-119.
33. Vrinten P, Hu Z, Munchinsky MA, Rowland G, Qiu X (2005) Two FAD3 desaturase genes control the level of linolenic acid in flax seed. Plant Physiol 139: 79-87.
34. Wu G, Truksa M, Datla N, Vrinten P, Bauer J, Zank T, Cirpus P, Heinz E, Qiu X (2005) Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat Biotechnol 23: 1013-1017.
35. Zank TK, Zähringer U, Beckmann C, Pohnert G, Boland W, Holtorf H, Reski R, Lerchl J, Heinz E (2002) Cloning and functional characterisation of an enzyme involved in the elongation of Δ6-polyunsaturated fatty acids from the moss Physcomitrella patens. Plant J 31: 255-268.