-
1
-
-
3142712905
-
n-3 Fatty acids and cardiovascular disease: evidence explained and mechanisms explored
-
Calder P.C. n-3 Fatty acids and cardiovascular disease: evidence explained and mechanisms explored. Clin Sci 107 (2004) 1-11
-
(2004)
Clin Sci
, vol.107
, pp. 1-11
-
-
Calder, P.C.1
-
2
-
-
1542300237
-
The metabolic syndrome
-
Nugent A.P. The metabolic syndrome. Nutr Bull 29 (2004) 36-43
-
(2004)
Nutr Bull
, vol.29
, pp. 36-43
-
-
Nugent, A.P.1
-
3
-
-
32944467555
-
Long-chain n-3 PUFA: plant v. marine sources
-
Williams C.M., and Burdge G. Long-chain n-3 PUFA: plant v. marine sources. Proc Nutr Soc 65 (2006) 42-50
-
(2006)
Proc Nutr Soc
, vol.65
, pp. 42-50
-
-
Williams, C.M.1
Burdge, G.2
-
4
-
-
10044253311
-
Enzymes for transgenic biosynthesis of long-chain polyunsaturated fatty acids
-
Huang Y.S., Pereira S., and Leonard A. Enzymes for transgenic biosynthesis of long-chain polyunsaturated fatty acids. Biochimie 86 (2004) 793-798
-
(2004)
Biochimie
, vol.86
, pp. 793-798
-
-
Huang, Y.S.1
Pereira, S.2
Leonard, A.3
-
5
-
-
0742324895
-
Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants
-
Sayanova O.V., and Napier J.A. Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry 65 (2004) 147-158
-
(2004)
Phytochemistry
, vol.65
, pp. 147-158
-
-
Sayanova, O.V.1
Napier, J.A.2
-
6
-
-
22144437034
-
Fatty acid desaturases from the microalga Thalassiosira pseudonana
-
Tonon T., Sayanova O., Michaelson L.V., Qing R., Harvey D., Larson T.R., Li Y., Napier J.A., and Graham I.A. Fatty acid desaturases from the microalga Thalassiosira pseudonana. FEBS J 272 (2005) 3401-3412
-
(2005)
FEBS J
, vol.272
, pp. 3401-3412
-
-
Tonon, T.1
Sayanova, O.2
Michaelson, L.V.3
Qing, R.4
Harvey, D.5
Larson, T.R.6
Li, Y.7
Napier, J.A.8
Graham, I.A.9
-
7
-
-
33645667607
-
Metabolic engineering of plants to produce very long-chain polyunsaturated fatty acids
-
Truksa M., Wu G., Vrinten P., and Qiu X. Metabolic engineering of plants to produce very long-chain polyunsaturated fatty acids. Transgenic Res 15 (2006) 131-137
-
(2006)
Transgenic Res
, vol.15
, pp. 131-137
-
-
Truksa, M.1
Wu, G.2
Vrinten, P.3
Qiu, X.4
-
8
-
-
0036000059
-
High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare
-
Hong L.H., Datla N., Reed D.W., Covello P.S., MacKenzie S.L., and Qiu X. High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare. Plant Physiol 129 (2002) 354-362
-
(2002)
Plant Physiol
, vol.129
, pp. 354-362
-
-
Hong, L.H.1
Datla, N.2
Reed, D.W.3
Covello, P.S.4
MacKenzie, S.L.5
Qiu, X.6
-
9
-
-
0030890581
-
5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco
-
5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco. Proc Natl Acad Sci USA 94 (1997) 4211-4216
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 4211-4216
-
-
Sayanova, O.1
Smith, M.A.2
Lapinskas, P.3
Stobart, A.K.4
Dobson, G.5
Christie, W.W.6
Shewry, P.R.7
Napier, J.A.8
-
10
-
-
0032491316
-
Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canola
-
Knutzon D.S., Thurmond J.M., Huang Y.S., Chaudhary S., Bobik Jr. E.G., Chan G.M., Kirchner S.J., and Mukerji P. Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canola. J Biol Chem 273 (1998) 29360-29366
-
(1998)
J Biol Chem
, vol.273
, pp. 29360-29366
-
-
Knutzon, D.S.1
Thurmond, J.M.2
Huang, Y.S.3
Chaudhary, S.4
Bobik Jr., E.G.5
Chan, G.M.6
Kirchner, S.J.7
Mukerji, P.8
-
11
-
-
2542627453
-
Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants
-
This study represents one of the first clear demonstrations that metabolic engineering of LC-PUFA production is feasible in higher plants. Constitutive expression of the relevant transgenes led to production of significant levels of LC-PUFAs in leaf tissue of Arabidopsis.
-
Qi B., Fraser T., Mugford S., Dobson G., Sayanova O., Butler J., Napier J., Stobart A., and Lazarus C. Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol 22 (2004) 739-745. This study represents one of the first clear demonstrations that metabolic engineering of LC-PUFA production is feasible in higher plants. Constitutive expression of the relevant transgenes led to production of significant levels of LC-PUFAs in leaf tissue of Arabidopsis.
-
(2004)
Nat Biotechnol
, vol.22
, pp. 739-745
-
-
Qi, B.1
Fraser, T.2
Mugford, S.3
Dobson, G.4
Sayanova, O.5
Butler, J.6
Napier, J.7
Stobart, A.8
Lazarus, C.9
-
12
-
-
10044222083
-
Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oils
-
This was the first demonstration of LC-PUFA production in seed oil. Seed-specific promoters were used to drive transgenes from marine alga and moss to achieve metabolic engineering of seed oil biosynthesis.
-
Abbadi A., Domergue F., Bauer J., Napier J., Welti R., Cirpus P., and Heinz E. Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oils. Plant Cell 16 (2004) 2734-2748. This was the first demonstration of LC-PUFA production in seed oil. Seed-specific promoters were used to drive transgenes from marine alga and moss to achieve metabolic engineering of seed oil biosynthesis.
-
(2004)
Plant Cell
, vol.16
, pp. 2734-2748
-
-
Abbadi, A.1
Domergue, F.2
Bauer, J.3
Napier, J.4
Welti, R.5
Cirpus, P.6
Heinz, E.7
-
13
-
-
33644747408
-
Progress towards the production of very long-chain polyunsaturated fatty acid in transgenic plants: plant metabolic engineering comes of age
-
Napier J., Haslam R., Caleron M., Michaelson L., Beaudoin F., and Sayanova O. Progress towards the production of very long-chain polyunsaturated fatty acid in transgenic plants: plant metabolic engineering comes of age. Physiol Plantarium 126 (2006) 398-406
-
(2006)
Physiol Plantarium
, vol.126
, pp. 398-406
-
-
Napier, J.1
Haslam, R.2
Caleron, M.3
Michaelson, L.4
Beaudoin, F.5
Sayanova, O.6
-
14
-
-
33645228559
-
5-fusion desaturase
-
Identification of acyl-exchange bottlenecks in the alternative Δ8-pathway. Also describes a variant form of a LC-PUFA desaturase.
-
5-fusion desaturase. FEBS Lett 580 (2006) 1946-1952. Identification of acyl-exchange bottlenecks in the alternative Δ8-pathway. Also describes a variant form of a LC-PUFA desaturase.
-
(2006)
FEBS Lett
, vol.580
, pp. 1946-1952
-
-
Sayanova, O.1
Haslam, R.2
Qi, B.3
Lazarus, C.M.4
Napier, J.A.5
-
15
-
-
23444444336
-
Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants
-
The authors demonstrate the accumulation of ARA and EPA to significant levels in transgenic B. juncea. The proof-of-concept synthesis of DHA is also described. This study represents some of the most sophisticated plant metabolic engineering to date, with up to nine transgenes being co-expressed to generate the LC-PUFA trait.
-
Wu G., Truksa M., Datla N., Vrinten P., Bauer J., Zank T., Cirpus P., Heinz E., and Qiu X. Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat Biotechnol 23 (2005) 1013-1017. The authors demonstrate the accumulation of ARA and EPA to significant levels in transgenic B. juncea. The proof-of-concept synthesis of DHA is also described. This study represents some of the most sophisticated plant metabolic engineering to date, with up to nine transgenes being co-expressed to generate the LC-PUFA trait.
-
(2005)
Nat Biotechnol
, vol.23
, pp. 1013-1017
-
-
Wu, G.1
Truksa, M.2
Datla, N.3
Vrinten, P.4
Bauer, J.5
Zank, T.6
Cirpus, P.7
Heinz, E.8
Qiu, X.9
-
16
-
-
34147165365
-
-
Kinney AJ: Production of very long chain polyunsaturated fatty acids in oilseeds. 2004; Patent WO 2004/071467 A2. A patent application that details the production of EPA and DHA in transgenic soybean seeds and somatic embryos. Represents the highest reported levels of these target LC-PUFAs
-
-
-
-
17
-
-
0030897513
-
Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling
-
Faergeman N.J., and Knudsen J. Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling. Biochem J 323 (1997) 1-12
-
(1997)
Biochem J
, vol.323
, pp. 1-12
-
-
Faergeman, N.J.1
Knudsen, J.2
-
18
-
-
22544452691
-
In vivo characterization of the first acyl-CoA Δ6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri
-
The functional characterisation of the first acyl-CoA desaturase from plants opened up the possibility for production of LC-PUFAs using acyl-CoA substrates for both desaturation and elongation, thereby avoiding the acyl-exchange problem.
-
Domergue F., Abbadi A., Zahringer U., Moreau H., and Heinz E. In vivo characterization of the first acyl-CoA Δ6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem J 389 (2005) 483-490. The functional characterisation of the first acyl-CoA desaturase from plants opened up the possibility for production of LC-PUFAs using acyl-CoA substrates for both desaturation and elongation, thereby avoiding the acyl-exchange problem.
-
(2005)
Biochem J
, vol.389
, pp. 483-490
-
-
Domergue, F.1
Abbadi, A.2
Zahringer, U.3
Moreau, H.4
Heinz, E.5
-
19
-
-
22344438841
-
Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil
-
The authors demonstrate the possibility of synthesizing EPA in transgenic plants via an acyl-CoA dependent route, also allowing the further synthesis of DHA.
-
Robert S.S., Singh S.P., Zhou X.R., Petrie J.R., Blackburn S.I., Mansour P.M., Nichols P.D., Liu Q., and Green A.G. Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil. Funct Plant Biol 32 (2005) 473-479. The authors demonstrate the possibility of synthesizing EPA in transgenic plants via an acyl-CoA dependent route, also allowing the further synthesis of DHA.
-
(2005)
Funct Plant Biol
, vol.32
, pp. 473-479
-
-
Robert, S.S.1
Singh, S.P.2
Zhou, X.R.3
Petrie, J.R.4
Blackburn, S.I.5
Mansour, P.M.6
Nichols, P.D.7
Liu, Q.8
Green, A.G.9
-
20
-
-
33745475386
-
Identification of bifunctional Δ12/omega 3 fatty acid desaturases for improving the ratio of omega 3 to omega 6 fatty acids in microbes and plants
-
Damude H.G., Zhang H., Farrall L., Ripp K.G., Tomb J.F., Hollerbach D., and Yadav N.S. Identification of bifunctional Δ12/omega 3 fatty acid desaturases for improving the ratio of omega 3 to omega 6 fatty acids in microbes and plants. Proc Natl Acad Sci USA 103 (2006) 9446-9451
-
(2006)
Proc Natl Acad Sci USA
, vol.103
, pp. 9446-9451
-
-
Damude, H.G.1
Zhang, H.2
Farrall, L.3
Ripp, K.G.4
Tomb, J.F.5
Hollerbach, D.6
Yadav, N.S.7
-
21
-
-
33846005156
-
A bi-functional Δ12, Δ15 desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids
-
Sayanova O., Haslam R., Guschina I., Lloyd D., Christie W.W., Harwood J.L., and Napier J.A. A bi-functional Δ12, Δ15 desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids. J Biol Chem 281 (2006) 36533-36541
-
(2006)
J Biol Chem
, vol.281
, pp. 36533-36541
-
-
Sayanova, O.1
Haslam, R.2
Guschina, I.3
Lloyd, D.4
Christie, W.W.5
Harwood, J.L.6
Napier, J.A.7
-
22
-
-
33751106179
-
Identification of Primula 'front-end' desaturases with distinct n-6 or n-3 substrate preferences
-
Sayanova O., Haslam R., Venegas-Caleron M., and Napier J.A. Identification of Primula 'front-end' desaturases with distinct n-6 or n-3 substrate preferences. Planta 224 (2006) 1269-1277
-
(2006)
Planta
, vol.224
, pp. 1269-1277
-
-
Sayanova, O.1
Haslam, R.2
Venegas-Caleron, M.3
Napier, J.A.4
-
23
-
-
33748930822
-
Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean
-
Eckert H., Lavallee B., Schweiger B.J., Kinney A.J., Cahoon E.B., and Clemente T. Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean. Planta 224 (2006) 1050-1057
-
(2006)
Planta
, vol.224
, pp. 1050-1057
-
-
Eckert, H.1
Lavallee, B.2
Schweiger, B.J.3
Kinney, A.J.4
Cahoon, E.B.5
Clemente, T.6
-
24
-
-
34147174665
-
-
Venegas-Caleron M, Beaudoin F, Sayanova O, Napier JA: Cotranscribed genes for long chain polyunsaturated fatty acid biosynthesis in the protozoon Perkinsus marinus contain a plant-like FAE1 3-ketoacyl-CoA synthase. J Biol Chem 2007, in press. The first example of an FAE1-like elongase involved in LC-PUFA biosynthesis, in this case as part of the alternative Δ8-pathway. Also reports the clustering of LC-PUFA biosynthetic activities.
-
-
-
-
25
-
-
14744267232
-
Relief for fish stocks: oceanic fatty acids in transgenic oilseeds
-
Domergue F., Abbadi A., and Heinz E. Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trends Plant Sci 10 (2005) 112-116
-
(2005)
Trends Plant Sci
, vol.10
, pp. 112-116
-
-
Domergue, F.1
Abbadi, A.2
Heinz, E.3
-
26
-
-
3543019724
-
Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis
-
Stahl U., Carlsson A.S., Lenman M., Dahlqvist A., Huang B., Banas W., Banas A., and Stymne S. Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol 135 (2004) 1324-1335
-
(2004)
Plant Physiol
, vol.135
, pp. 1324-1335
-
-
Stahl, U.1
Carlsson, A.S.2
Lenman, M.3
Dahlqvist, A.4
Huang, B.5
Banas, W.6
Banas, A.7
Stymne, S.8
-
27
-
-
0035128174
-
Technical advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues
-
Larson T.R., and Graham I.A. Technical advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues. Plant J 25 (2001) 115-125
-
(2001)
Plant J
, vol.25
, pp. 115-125
-
-
Larson, T.R.1
Graham, I.A.2
-
28
-
-
33748514364
-
Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning
-
Ejsing C.S., Duchoslav E., Sampaio J., Simons K., Bonner R., Thiele C., Ekroos K., and Shevchenko A. Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning. Anal Chem 78 (2006) 6202-6214
-
(2006)
Anal Chem
, vol.78
, pp. 6202-6214
-
-
Ejsing, C.S.1
Duchoslav, E.2
Sampaio, J.3
Simons, K.4
Bonner, R.5
Thiele, C.6
Ekroos, K.7
Shevchenko, A.8
-
29
-
-
33749263895
-
Tung tree DGAT1 and DGAT2 have non-redundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum
-
This article introduces the possibility that distinct subdomains in the ER may be responsible for the synthesis of membrane-associated and storage lipids. If correct, the challenge will be to localize relevant heterologous proteins specifically to the subdomain for oil biosynthesis.
-
Shockey J.M., Gidda S.K., Chapital D.C., Kuan J.C., Dhanoa P.K., Bland J.M., Rothstein J.S., Mullen R.T., and Dyer J.M. Tung tree DGAT1 and DGAT2 have non-redundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18 (2006) 2294-2313. This article introduces the possibility that distinct subdomains in the ER may be responsible for the synthesis of membrane-associated and storage lipids. If correct, the challenge will be to localize relevant heterologous proteins specifically to the subdomain for oil biosynthesis.
-
(2006)
Plant Cell
, vol.18
, pp. 2294-2313
-
-
Shockey, J.M.1
Gidda, S.K.2
Chapital, D.C.3
Kuan, J.C.4
Dhanoa, P.K.5
Bland, J.M.6
Rothstein, J.S.7
Mullen, R.T.8
Dyer, J.M.9
-
30
-
-
33751161853
-
Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis
-
Robenek H., Hofnagel O., Buers I., Robenek M.J., Troyer D., and Severs N.J. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis. J Cell Sci 119 (2006) 4215-4224
-
(2006)
J Cell Sci
, vol.119
, pp. 4215-4224
-
-
Robenek, H.1
Hofnagel, O.2
Buers, I.3
Robenek, M.J.4
Troyer, D.5
Severs, N.J.6
-
31
-
-
33748757743
-
Co-localization of SCD1 and DGAT2: implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis
-
Man W.C., Miyazaki M., Chu K., and Ntambi J. Co-localization of SCD1 and DGAT2: implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis. J Lipid Res 47 (2006) 1928-1939
-
(2006)
J Lipid Res
, vol.47
, pp. 1928-1939
-
-
Man, W.C.1
Miyazaki, M.2
Chu, K.3
Ntambi, J.4
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