Peptide conjugated phosphorodiamidate morpholino oligomers increase survival of mice challenged with ames bacillus anthracis

Nucleic Acid Therapeutics

Volumn 22, Issue 5, 2012, Pages 316-322

  Panchal, Rekha G ^a   Geller, Bruce L ^b,c   Mellbye, Brett ^b,c   Lane, Douglas ^d   Iversen, Patrick L ^b,c   Bavari, Sina ^a  

^a UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^b AVI BIOPHARMA INC   (United States)

^c OREGON STATE UNIVERSITY   (United States)

^d SAIC FREDERICK INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATIONIC PEPTIDE; CIPROFLOXACIN; MEMBRANE PENETRATING PEPTIDE; MORPHOLINO OLIGONUCLEOTIDE; PEPTIDE; UNCLASSIFIED DRUG;

ACYL CARRIER PROTEIN GENE; AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTHRAX; ANTIBACTERIAL ACTIVITY; ANTIBIOTIC SENSITIVITY; APOPTOSIS; ARTICLE; BACILLUS ANTHRACIS; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; CELL VIABILITY; CONTROLLED STUDY; DRUG CONJUGATION; DRUG EFFICACY; GENE TARGETING; GROWTH INHIBITION; GYRASE A GENE; IN VITRO STUDY; MACROPHAGE; MINIMUM INHIBITORY CONCENTRATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; START CODON; SURVIVAL RATE;

AMINO ACID SEQUENCE; ANIMALS; ANTHRAX; ANTI-BACTERIAL AGENTS; BACILLUS ANTHRACIS; BASE SEQUENCE; CELL LINE; CELL SURVIVAL; CELL-PENETRATING PEPTIDES; CIPROFLOXACIN; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; MACROPHAGES; MICE; MICE, INBRED C57BL; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MORPHOLINOS;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); MUS; POSIBACTERIA;

EID: 84870355586     PISSN: 21593337     EISSN: None     Source Type: Journal    
DOI: 10.1089/nat.2012.0362     Document Type: Article

References (29)

1. BAI, H., ZHOU, Y., HOU, Z., XUE, X., MENG, J., and LUO, X. (2011). Targeting bacterial RNA polymerase: promises for future antisense antibiotics development. Infect Disord. Drug Targets 11, 175-187.
2. BROOK, I. (2002). The prophylaxis and treatment of anthrax. Int. J. Antimicrob. Agents 20, 320-325. (Pubitemid 35300427)
3. BROOK, I., ELLIOTT, T.B., PRYOR, H.I., 2nd, SAUTTER, T.E., GNADE, B.T., THAKAR, J.H., and KNUDSON, G.B. (2001). In vitro resistance of Bacillus anthracis Sterne to doxycycline, macrolides, and quinolones. Int. J. Antimicrob. Agents 18, 559-562. (Pubitemid 33152750)
4. CHOE, C.H., BOUHAOUALA, S.S., BROOK, I., ELLIOT, T.B., and KNUDSON, G.B. (2000). In vitro development of resistance to ofloxacin and doxycycline in Bacillus anthracis Sterne. Antimicrob. Agents Chemother. 44, 1766. (Pubitemid 30340839)
5. CHRISTEN, B., ABELIUK, E., COLLIER, J.M., KALOGERAKI, V.S., PASSARELLI, B., COLLER, J.A., FERO, M.J., MCADAMS, H.H., and SHAPIRO, L. (2011). The essential genome of a bacterium. Mol. Syst. Biol. 7, 528.
6. DEERE, J., IVERSEN, P., and GELLER, B.L. (2005). Antisense phosphorodiamidate morpholino oligomer length and target position effects on gene-specific inhibition in Escherichia coli. Antimicrob. Agents Chemother. 49, 249-255. (Pubitemid 40065799)
7. FOX, M.J., REEVES, M.D., and WELLER, D.D. (2009). Methods of synthesis of morpholino oligomers. Publication No. WO/2009/064471. World Intellectual Property Organization, Geneva, Switzerland.
8. FRIEDLANDER, A.M., BHATNAGAR, R., LEPPLA, S.H., JOHNSON, L., and SINGH, Y. (1993). Characterization of macrophage sensitivity and resistance to anthrax lethal toxin. Infect. Immun. 61, 245-252. (Pubitemid 23015423)
9. GELLER, B.L., DEERE, J., TILLEY, L., and IVERSEN, P.L. (2005). Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis. J. Antimicrob. Chemother. 55, 983-988. (Pubitemid 40883185)
10. GELLER, B.L., DEERE, J.D., STEIN, D.A., KROEKER, A.D., MOULTON, H.M., and IVERSEN, P.L. (2003). Inhibition of gene expression in Escherichia coli by antisense phosphorodiamidate morpholino oligomers. Antimicrob. Agents Chemother. 47, 3233-3239. (Pubitemid 37229583)
11. GIL, R., SILVA, F.J., PERETO, J., and MOYA, A. (2004). Determination of the core of a minimal bacterial gene set. Microbiol. Mol. Biol. Rev. 68, 518-537. (Pubitemid 39238281)
12. GOH, S., BOBEREK, J.M., NAKASHIMA, N., STACH, J., and GOOD, L. (2009). Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli. PLoS One 4, e6061.
13. GOOD, L., AWASTHI, S.K., DRYSELIUS, R., LARSSON, O., and NIELSEN, P.E. (2001). Bactericidal antisense effects of peptide-PNA conjugates. Nat. Biotechnol. 19, 360-364. (Pubitemid 32275346)
14. GREENBERG, D.E., MARSHALL-BATTY, K.R., BRINSTER, L.R., ZAREMBER, K.A., SHAW, P.A., MELLBYE, B.L., IVERSEN, P.L., HOLLAND, S.M., and GELLER, B.L. (2010). Antisense phosphorodiamidate morpholino oligomers targeted to an essential gene inhibit Burkholderia cepacia complex. J. Infect. Dis. 201, 1822-1830.
15. IVERSEN, P.L. (2001). Phosphorodiamidate morpholino oligomers: favorable properties for sequence-specific gene inactivation. Curr. Opin. Mol. Ther. 3, 235-238. (Pubitemid 32624571)
16. MELLBYE, B.L., PUCKETT, S.E., TILLEY, L.D., IVERSEN, P.L., and GELLER, B.L. (2009). Variations in amino acid composition of antisense peptide-phosphorodiamidate morpholino oligomer affect potency against Escherichia coli in vitro and in vivo. Antimicrob. Agents Chemother. 53, 525-530.
17. MELLBYE, B.L., WELLER, D.D., HASSINGER, J.N., REEVES, M.D., LOVEJOY, C.E., IVERSEN, P.L., and GELLER, B.L. (2010). Cationic phosphorodiamidate morpholino oligomers efficiently prevent growth of Escherichia coli in vitro and in vivo. J. Antimicrob. Chemother. 65, 98-106.
18. MOULTON, H.M., NELSON, M.H., HATLEVIG, S.A., REDDY, M.T., and IVERSEN, P.L. (2004). Cellular uptake of antisense morpholino oligomers conjugated to arginine-rich peptides. Bioconjug. Chem. 15, 290-299. (Pubitemid 38365188)
19. NATIONAL COMMITTEE FOR CLINICAL LABORATORY STANDARDS (NCCLS). (2003). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, M7-A6. (National Committee for Clinical Laboratory Standards, Wayne, PA).
20. NATIONAL RESEARCH COUNCIL. (1996). Guide for the care and use of laboratory animals. (The National Academies Press, Washington, DC).
21. NIELSEN, P.E. (2010). Peptide nucleic acids (PNA) in chemical biology and drug discovery. Chem. Biodivers. 7, 786-804.
22. PRICE, L.B., VOGLER, A., PEARSON, T., BUSCH, J.D., SCHUPP, J.M., and KEIM, P. (2003). In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin. Antimicrob. Agents Chemother. 47, 2362-2365. (Pubitemid 36753604)
23. SUMMERTON, J.E., and WELLER, D.D. (1993). Uncharged morpholino-based polymers having phosphorous containing chiral intersubunit linkages. U.S. Patent 5,185,444, Feb 9, 1993.
24. TILLEY, L.D., HINE, O.S., KELLOGG, J.A., HASSINGER, J.N., WELLER, D.D., IVERSEN, P.L., and GELLER, B.L. (2006). Gene-specific effects of antisense phosphorodiamidate morpholino oligomer-peptide conjugates on Escherichia coli and Salmonella enterica serovar typhimurium in pure culture and in tissue culture. Antimicrob. Agents Chemother. 50, 2789-2796. (Pubitemid 44198702)
25. TILLEY, L.D., MELLBYE, B.L., PUCKETT, S.E., IVERSEN, P.L., and GELLER, B.L. (2007). Antisense peptide-phosphorodiamidate morpholino oligomer conjugate: dose-response in mice infected with Escherichia coli. J Antimicrob. Chemother. 59, 66-73. (Pubitemid 46017902)
26. VAARA, M., and PORRO, M. (1996). Group of peptides that act synergistically with hydrophobic antibiotics against gramnegative enteric bacteria. Antimicrob. Agents Chemother. 40, 1801-1805. (Pubitemid 26277796)
27. VELLANOWETH, R.L., and RABINOWITZ, J.C. (1992). The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Mol. Microbiol. 6, 1105-1114.
28. WELLER D.D., and HASSINGER J.N. (2008). Oliognucleotide analogs having cationic intersubnit linkages. Publication No. WO/2008/03612. World Intellectual Property Organization, Geneva, Switzerland.
29. WESOLOWSKI, D., TAE, H.S., GANDOTRA, N., LLOPIS, P., SHEN, N., and ALTMAN, S. (2011). Basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes. Proc. Natl. Acad. Sci. U. S. A. 108, 16582-16587.