Genomic characterization of a three-dimensional skin model following exposure to ionizing radiation

Journal of Radiation Research

Volumn 53, Issue 6, 2012, Pages 860-875

  Yunis, Reem ^a   Albrecht, Huguette ^a   Kalanetra, Karen M ^a   Wu, Shiquan ^a   Rocke, David M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

EpiDermFT; gene expression; low dose ionizing radiation; microarray

Indexed keywords

CELL CULTURE; CELL DEATH; CELL SIGNALING; HISTOLOGY; IONIZING RADIATION; MOLECULAR BIOLOGY; RADIATION SHIELDING; TISSUE;

CELL CYCLE; EPIDERMFT; GENES EXPRESSION; GENOMIC CHARACTERIZATION; GENOMIC RESPONSE; LOW DOSE; LOW DOSE IONIZING RADIATION; MODEL-FOLLOWING; POSTIRRADIATION; SKIN MODEL;

GENE EXPRESSION;

EID: 84870028175     PISSN: 04493060     EISSN: 13499157     Source Type: Journal    
DOI: 10.1093/jrr/rrs063     Document Type: Article

References (43)

1. Redpath JL. Radiation-induced neoplastic transformation in vitro: evidence for a protective effect at low doses of low LET radiation. Cancer Metastasis Rev 2004;23:333-9. (Pubitemid 38881816)
2. Preston DL, Pierce DA, Shimizu Y et al. Dose response and temporal patterns of radiation associated solid cancer risks. Health Phys 2003;85:43-6. (Pubitemid 36706039)
3. Preston RJ. The LNT model is the best we can do-today. J Radiol Prot 2003;23:263-8. (Pubitemid 37254165)
4. Brenner DJ, Doll R, Goodhead DT et al. Cancer risks attributable to low doses of ionizing radiation: Assessing what we really know. Proc Natl Acad Sci USA 2003;100:13761-6. (Pubitemid 37499140)
5. NRC (National Research Council). 2006. Health Risks from Exposure to Low Levels of Ionizing Radiation. BEIR VII Phase 2. Washington, DC: National Academy Press. Available from: http://www.nap.edu/openbook.php?record-id= 11340&page=1
6. Royal HD. Effects of low level radiation-what's new? Semin Nucl Med 2008;38:392-402. (Pubitemid 352033562)
7. Prise KM. New advances in radiation biology. Occup Med 2006;56:156-61.
8. Karagiannis TC, El-Osta A. Double-strand breaks: signaling pathways and repair mechanisms. Cell Mol Life Sci 2006;61:2137-47.
9. Sowa MB, Chrisler WB, Zens KD et al. Three-dimensional culture conditions lead to decreased radiation induced cytotoxicity in human mammary epithelial cells. Mutat Res 2006;687:78-83.
10. Storch K, Eke I, Borgmann K et al. Three-dimensional cell growth confers radioresistance by chromatin density modification. Cancer Res 2010;70:3925-34.
11. Hayden PJ, Stolper G, Cooney C et al. Healing of dermal wounds in the EpiDerm-FT™ in vitro human skin model: An animal alternative for wound healing issues. The Toxicologist 2008;102:69.
12. Moore JO, Stebbins WG, Guevara D et al. Human skin equivalent: A reliable in vitro model for carcinogenic experiments. J Invest Dermatol 2004;122:A23.
13. Moore JO, Wang Y, Stebbins WG et al. Photoprotective effect of isoflavone genistein on ultraviolet B-induced pyrimidine dimer formation and PCNA expression in human reconstituted skin and its implications in dermatology and prevention of cutaneous carcinogenesis. Carcinogenesis 2006;27:1627-35. (Pubitemid 44288052)
14. Afaq F, Zaid MA, Khan N et al. Protective effects of pomegranate-derived products on UVB mediated damage in human reconstituted skin. Exp Dermatol 2009;18:553-61.
15. Flockhart RJ, Diffey BL, Farr PM et al. NFAT regulates induction of COX-2 and apoptosis of keratinocytes in response to ultraviolet radiation exposure. FASEB J 2008;22:4218-27.
16. Murray AR, Kisin E, Leonar SS et al. Oxidative stress and inflammatory response in dermal toxicity of single-walled carbon nanotubes. Toxicology 2009;257:161-71.
17. Su Y, Meador JA, Geard CR et al. Analysis of ionizing radiation-induced DNA damage and repair in threedimensional human skin model system. Exp Dermatol 2010; 19:e16-e22.
18. Hei TK, Ballas LK, Brenner DJ et al. Advances in radiobiological studies using a microbeam. J Radiat Res 2009;50 Suppl A:A7-12.
19. Schmid TE, Dollinger G, Hable V et al. Relative biological effectiveness of pulsed and continuous 20 MeV protons for micronucleus induction in 3D human reconstructed skin tissue. Radiother Oncol 2010;95:66-72.
20. Miller JH, Suleiman A, Chrisler WB et al. Simulation of electron-beam irradiation of skin tissue model. Radiat Res 2011;175:113-8.
21. Belyakov OV, Mitchell SA, Parikh D et al. Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away. Proc Natl Acad Sci USA 2005;102:14203-8. (Pubitemid 41429676)
22. Sedelnikova OA, Nakamura A, Kovalchuk O et al. DNA double-strand breaks form in bystander cells after microbeam irradiation of three-dimensional human tissue models. Cancer Res 2007;67:4295-302. (Pubitemid 46815077)
23. Curren RD, Mun GC, Gibson DP et al. Development of a method for assessing micronucleus induction in a 3D human skin model (EpiDerm). Mutat Res 2006;607:192-204. (Pubitemid 44107786)
24. Netzlaff F, Lehr CM, Wertz PW et al. The human epidermis models EpiSkin, SkinEthic and EpiDerm: an evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport. Eur J Pharm Biopharm 2005;60:167-78. (Pubitemid 40804559)
25. Rocke DM, Durbin BP. A model for measurement errors for gene expression arrays. J Comp Biol 2001;8:557-69. (Pubitemid 34053496)
26. Durbin BP, Hardin J, Hawkins D et al. A variance-stabilizing transformation for gene expression microarray data. Bioinformatics 2002;18:S105-10. (Pubitemid 41185643)
27. Durbin BP, Rocke DM. Estimation of transformation parameters for microarray data. Bioinformatics 2003;19:1360-7. (Pubitemid 36939813)
28. Rocke DM, Durbin BP. Approximate variance-stabilizing transformation parameters for microarray data. Bioinformatics 2003;19:966-72. (Pubitemid 36675817)
29. Rocke DM. Design and analysis of experiments with high throughput biological assay data. Semin Cell Dev Biol 2004;15:703-13. (Pubitemid 39550250)
30. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc, Series B 1995;57: 289-300.
31. Yin E, Nelson DO, Coleman MA et al. Gene expression changes in mouse brain after exposure to low-dose ionizing radiation. Int J Rad Biol 2003;79:759-75. (Pubitemid 37468143)
32. JJin YW, Na YJ, Lee YJ et al. Comprehensive analysis of time- and dose-dependent patterns of gene expression in a human mesenchymal stem cell line exposed to low dose ionizing radiation. Oncol Rep 2008;19:135-44.
33. Mezentsev A, Amundson SA. Global gene expression responses to low- or high-dose radiation in a human threedimensional tissue model. Radiat Res 2011;175:677-88.
34. Ding LH, Shingyoji M, Chen F et al. Gene expression profiles of normal human fibroblasts after exposure to ionizing radiation: a comparative study of low and high doses. Radiat Res 2005;164:17-26. (Pubitemid 40885226)
35. Ghandhi SA, Ming L, Ivanov VN et al. Regulation of early signaling and gene expression in the alpha-particle and bystander response of IMR-90 human fibroblasts. BMC Med Genomics 2010;3:31.
36. Ghandhi SA, Yaghoubian B, Amundson SA. Global gene expression analyses of bystander and alpha particle irradiated normal human lung fibroblasts: synchronous and differential responses. BMC Med Genomics 2008;1:63.
37. Lukas J, Lukas C, Bartek J. Mammalian cell cycle checkpoints: signalling pathways and their organization in space and time. DNA Repair 2004;3:997-1007. (Pubitemid 38997942)
38. Zhou T, Chou JW, Simpson DA et al. Profiles of global gene expression in ionizing radiation-damaged human diploid fibroblasts reveal synchronization behind the G1 checkpoint in a G0-like state of quiescence. Environ Health Perspect 2006;114:553-9.
39. Kim CS, Kim JK, Nam SY et al. Low-dose radiation stimulates the proliferation of normal human lung fibroblasts via a transient activation of Raf and Akt. Mol Cells 2007; 24:424-30.
40. Suzuki K, Nakashima M, Yamashita S. Dynamics of ionizing radiation-induced DNA damage response in reconstituted threedimensional human skin tissue. Radiat Res 2010;174:415-23.
41. Albrecht H, Durbin-Johnson B, Yunis R et al. Transcriptional response of ex vivo human skin to ionizing radiation: comparison between low- and high-dose effects. Radiat Res 2012;177:69-83.
42. Goldberg Z, Rocke DM, Schwietert C et al. Human in vivo dose-response to controlled, low dose low linear energy transfer ionizing radiation exposure. Clin Cancer Res 2006;12: 3723-9. (Pubitemid 44000253)
43. Berglund SR, Rocke DM, Dai J et al. Transient genome-wide transcriptional response to low-dose ionizing radiation in vivo in humans. Int J Radiat Oncol Biol Phys 2008;70:229-34. (Pubitemid 350251058)